Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01360

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01350 and BWI76_RS01355 are separated by 44 nucleotidesBWI76_RS01355 and BWI76_RS01360 are separated by 126 nucleotidesBWI76_RS01360 and BWI76_RS01365 are separated by 9 nucleotides BWI76_RS01350: BWI76_RS01350 - NAD(P)H-flavin reductase, at 276,280 to 276,981 _RS01350 BWI76_RS01355: BWI76_RS01355 - hypothetical protein, at 277,026 to 277,337 _RS01355 BWI76_RS01360: BWI76_RS01360 - acetyl-CoA C-acyltransferase FadA, at 277,464 to 278,627 _RS01360 BWI76_RS01365: BWI76_RS01365 - multifunctional fatty acid oxidation complex subunit alpha, at 278,637 to 280,826 _RS01365 Position (kb) 277 278 279Strain fitness (log2 ratio) -3 -2 -1 0 1at 276.740 kb on + strand, within BWI76_RS01350at 277.128 kb on - strand, within BWI76_RS01355at 277.128 kb on - strand, within BWI76_RS01355at 277.174 kb on + strand, within BWI76_RS01355at 277.174 kb on + strand, within BWI76_RS01355at 277.182 kb on - strand, within BWI76_RS01355at 277.213 kb on + strand, within BWI76_RS01355at 277.221 kb on - strand, within BWI76_RS01355at 277.252 kb on + strand, within BWI76_RS01355at 277.277 kb on - strandat 277.306 kb on - strandat 277.344 kb on + strandat 277.426 kb on - strandat 277.458 kb on - strandat 277.470 kb on - strandat 277.497 kb on + strandat 277.545 kb on - strandat 277.545 kb on - strandat 277.562 kb on + strandat 277.602 kb on + strand, within BWI76_RS01360at 277.602 kb on + strand, within BWI76_RS01360at 277.652 kb on - strand, within BWI76_RS01360at 277.814 kb on - strand, within BWI76_RS01360at 277.907 kb on + strand, within BWI76_RS01360at 277.933 kb on + strand, within BWI76_RS01360at 278.006 kb on + strand, within BWI76_RS01360at 278.022 kb on + strand, within BWI76_RS01360at 278.103 kb on - strand, within BWI76_RS01360at 278.137 kb on - strand, within BWI76_RS01360at 278.446 kb on - strand, within BWI76_RS01360at 278.446 kb on - strand, within BWI76_RS01360at 278.446 kb on - strand, within BWI76_RS01360at 278.803 kb on + strandat 278.894 kb on + strand, within BWI76_RS01365at 278.979 kb on + strand, within BWI76_RS01365at 279.102 kb on - strand, within BWI76_RS01365at 279.293 kb on + strand, within BWI76_RS01365at 279.475 kb on - strand, within BWI76_RS01365at 279.514 kb on - strand, within BWI76_RS01365at 279.546 kb on + strand, within BWI76_RS01365

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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276,740 + BWI76_RS01350 0.66 -3.1
277,128 - BWI76_RS01355 0.33 -1.2
277,128 - BWI76_RS01355 0.33 -0.1
277,174 + BWI76_RS01355 0.47 -1.4
277,174 + BWI76_RS01355 0.47 -0.3
277,182 - BWI76_RS01355 0.50 +1.6
277,213 + BWI76_RS01355 0.60 +0.2
277,221 - BWI76_RS01355 0.62 -0.3
277,252 + BWI76_RS01355 0.72 +1.7
277,277 - +1.1
277,306 - -0.4
277,344 + -0.3
277,426 - +0.6
277,458 - +0.9
277,470 - +0.4
277,497 + -0.7
277,545 - -2.5
277,545 - +0.3
277,562 + +1.2
277,602 + BWI76_RS01360 0.12 -2.5
277,602 + BWI76_RS01360 0.12 +0.1
277,652 - BWI76_RS01360 0.16 +0.7
277,814 - BWI76_RS01360 0.30 -0.8
277,907 + BWI76_RS01360 0.38 -0.2
277,933 + BWI76_RS01360 0.40 -0.2
278,006 + BWI76_RS01360 0.47 +1.2
278,022 + BWI76_RS01360 0.48 -0.1
278,103 - BWI76_RS01360 0.55 +0.1
278,137 - BWI76_RS01360 0.58 -2.1
278,446 - BWI76_RS01360 0.84 +0.1
278,446 - BWI76_RS01360 0.84 -1.3
278,446 - BWI76_RS01360 0.84 -1.9
278,803 + -1.0
278,894 + BWI76_RS01365 0.12 -1.9
278,979 + BWI76_RS01365 0.16 -0.7
279,102 - BWI76_RS01365 0.21 +0.6
279,293 + BWI76_RS01365 0.30 -3.7
279,475 - BWI76_RS01365 0.38 +0.5
279,514 - BWI76_RS01365 0.40 +0.4
279,546 + BWI76_RS01365 0.42 -0.5

Or see this region's nucleotide sequence