Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00110 and BWI76_RS00115 are separated by 164 nucleotides BWI76_RS00115 and BWI76_RS00120 are separated by 41 nucleotides BWI76_RS00120 and BWI76_RS00125 are separated by 54 nucleotides
BWI76_RS00110: BWI76_RS00110 - adenine permease PurP, at 20,859 to 22,199
_RS00110
BWI76_RS00115: BWI76_RS00115 - 6-phosphogluconate phosphatase, at 22,364 to 23,029
_RS00115
BWI76_RS00120: BWI76_RS00120 - glucosamine-6-phosphate deaminase, at 23,071 to 23,808
_RS00120
BWI76_RS00125: BWI76_RS00125 - putative xylanase, at 23,863 to 25,029
_RS00125
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 21.459 kb on + strand, within BWI76_RS00110 at 21.538 kb on + strand, within BWI76_RS00110 at 21.578 kb on - strand, within BWI76_RS00110 at 21.737 kb on + strand, within BWI76_RS00110 at 21.957 kb on - strand, within BWI76_RS00110 at 22.121 kb on - strand at 22.125 kb on - strand at 22.130 kb on - strand at 22.416 kb on - strand at 22.506 kb on - strand, within BWI76_RS00115 at 22.559 kb on - strand, within BWI76_RS00115 at 22.675 kb on - strand at 22.686 kb on + strand, within BWI76_RS00115 at 22.694 kb on - strand, within BWI76_RS00115 at 22.854 kb on - strand, within BWI76_RS00115 at 22.890 kb on - strand, within BWI76_RS00115 at 23.039 kb on + strand at 23.076 kb on - strand at 23.081 kb on - strand at 23.133 kb on + strand at 23.170 kb on - strand, within BWI76_RS00120 at 23.386 kb on - strand, within BWI76_RS00120 at 23.386 kb on - strand, within BWI76_RS00120 at 23.448 kb on + strand, within BWI76_RS00120 at 23.562 kb on + strand, within BWI76_RS00120 at 23.636 kb on + strand, within BWI76_RS00120 at 23.705 kb on - strand, within BWI76_RS00120 at 23.732 kb on + strand, within BWI76_RS00120 at 23.743 kb on - strand at 23.846 kb on - strand at 23.997 kb on - strand, within BWI76_RS00125 at 23.998 kb on - strand, within BWI76_RS00125
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 21,459 + BWI76_RS00110 0.45 +1.3 21,538 + BWI76_RS00110 0.51 -4.2 21,578 - BWI76_RS00110 0.54 -0.2 21,737 + BWI76_RS00110 0.65 -2.0 21,957 - BWI76_RS00110 0.82 +0.4 22,121 - +0.7 22,125 - +1.2 22,130 - -0.2 22,416 - +1.1 22,506 - BWI76_RS00115 0.21 -0.4 22,559 - BWI76_RS00115 0.29 -2.4 22,675 - +1.7 22,686 + BWI76_RS00115 0.48 +0.3 22,694 - BWI76_RS00115 0.50 -0.4 22,854 - BWI76_RS00115 0.74 -3.9 22,890 - BWI76_RS00115 0.79 -0.8 23,039 + -0.4 23,076 - -1.2 23,081 - +0.5 23,133 + -1.1 23,170 - BWI76_RS00120 0.13 -1.1 23,386 - BWI76_RS00120 0.43 -0.6 23,386 - BWI76_RS00120 0.43 -0.4 23,448 + BWI76_RS00120 0.51 -0.4 23,562 + BWI76_RS00120 0.67 +0.0 23,636 + BWI76_RS00120 0.77 -2.4 23,705 - BWI76_RS00120 0.86 -0.3 23,732 + BWI76_RS00120 0.90 -1.5 23,743 - -0.7 23,846 - -1.6 23,997 - BWI76_RS00125 0.11 -0.2 23,998 - BWI76_RS00125 0.12 -0.9
Or see this region's nucleotide sequence