Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1212 and Dshi_1213 are separated by 41 nucleotides Dshi_1213 and Dshi_1214 overlap by 4 nucleotides Dshi_1214 and Dshi_1215 overlap by 1 nucleotides Dshi_1215 and Dshi_1216 are separated by 98 nucleotides
Dshi_1212: Dshi_1212 - carbon-monoxide dehydrogenase, large subunit (RefSeq), at 1,250,367 to 1,252,787
_1212
Dshi_1213: Dshi_1213 - ATPase associated with various cellular activities AAA_5 (RefSeq), at 1,252,829 to 1,253,737
_1213
Dshi_1214: Dshi_1214 - VWA containing CoxE family protein (RefSeq), at 1,253,734 to 1,254,912
_1214
Dshi_1215: Dshi_1215 - protein of unknown function DUF182 (RefSeq), at 1,254,912 to 1,255,724
_1215
Dshi_1216: Dshi_1216 - 2-dehydropantoate 2-reductase (RefSeq), at 1,255,823 to 1,256,800
_1216
Position (kb)
1253
1254
1255 Strain fitness (log2 ratio)
-2
-1
0
1 at 1252.808 kb on + strand at 1252.815 kb on - strand at 1252.816 kb on - strand at 1252.863 kb on - strand at 1252.924 kb on + strand, within Dshi_1213 at 1253.021 kb on + strand, within Dshi_1213 at 1253.063 kb on - strand, within Dshi_1213 at 1253.322 kb on + strand, within Dshi_1213 at 1253.340 kb on - strand, within Dshi_1213 at 1253.621 kb on - strand, within Dshi_1213 at 1253.706 kb on - strand at 1253.818 kb on + strand at 1253.819 kb on - strand at 1253.883 kb on - strand, within Dshi_1214 at 1253.913 kb on - strand, within Dshi_1214 at 1253.934 kb on + strand, within Dshi_1214 at 1254.005 kb on - strand, within Dshi_1214 at 1254.005 kb on - strand, within Dshi_1214 at 1254.005 kb on - strand, within Dshi_1214 at 1254.039 kb on - strand, within Dshi_1214 at 1254.476 kb on - strand, within Dshi_1214 at 1254.527 kb on + strand, within Dshi_1214 at 1254.527 kb on + strand, within Dshi_1214 at 1254.528 kb on - strand, within Dshi_1214 at 1254.535 kb on - strand, within Dshi_1214 at 1254.535 kb on - strand, within Dshi_1214 at 1254.619 kb on + strand, within Dshi_1214 at 1254.867 kb on - strand at 1254.882 kb on - strand at 1255.042 kb on + strand, within Dshi_1215 at 1255.094 kb on - strand, within Dshi_1215 at 1255.094 kb on - strand, within Dshi_1215 at 1255.158 kb on + strand, within Dshi_1215 at 1255.158 kb on + strand, within Dshi_1215 at 1255.200 kb on + strand, within Dshi_1215 at 1255.443 kb on + strand, within Dshi_1215 at 1255.610 kb on + strand, within Dshi_1215
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 1,252,808 + +0.1 1,252,815 - -1.0 1,252,816 - +0.3 1,252,863 - +0.6 1,252,924 + Dshi_1213 0.10 -0.3 1,253,021 + Dshi_1213 0.21 +0.3 1,253,063 - Dshi_1213 0.26 +0.3 1,253,322 + Dshi_1213 0.54 +0.3 1,253,340 - Dshi_1213 0.56 +0.6 1,253,621 - Dshi_1213 0.87 +0.2 1,253,706 - +0.6 1,253,818 + +0.6 1,253,819 - -0.2 1,253,883 - Dshi_1214 0.13 -0.2 1,253,913 - Dshi_1214 0.15 -0.8 1,253,934 + Dshi_1214 0.17 -0.8 1,254,005 - Dshi_1214 0.23 -0.2 1,254,005 - Dshi_1214 0.23 +0.3 1,254,005 - Dshi_1214 0.23 +0.2 1,254,039 - Dshi_1214 0.26 -0.4 1,254,476 - Dshi_1214 0.63 +0.6 1,254,527 + Dshi_1214 0.67 -0.9 1,254,527 + Dshi_1214 0.67 +0.2 1,254,528 - Dshi_1214 0.67 +0.5 1,254,535 - Dshi_1214 0.68 +0.2 1,254,535 - Dshi_1214 0.68 -0.3 1,254,619 + Dshi_1214 0.75 -0.0 1,254,867 - -0.5 1,254,882 - +1.3 1,255,042 + Dshi_1215 0.16 +0.6 1,255,094 - Dshi_1215 0.22 +0.1 1,255,094 - Dshi_1215 0.22 -2.0 1,255,158 + Dshi_1215 0.30 -1.2 1,255,158 + Dshi_1215 0.30 -0.0 1,255,200 + Dshi_1215 0.35 -0.1 1,255,443 + Dshi_1215 0.65 +0.3 1,255,610 + Dshi_1215 0.86 -0.3
Or see this region's nucleotide sequence