Experiment: Tween 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2574 and HP15_2575 are separated by 230 nucleotides HP15_2575 and HP15_2576 overlap by 10 nucleotides
HP15_2574: HP15_2574 - alkane 1-monooxygenase, at 2,721,791 to 2,723,059
_2574
HP15_2575: HP15_2575 - enoyl-CoA hydratase/isomerase family protein carnitinyl-CoA, at 2,723,290 to 2,724,078
_2575
HP15_2576: HP15_2576 - acyl-CoA dehydrogenase domain protein, at 2,724,069 to 2,725,871
_2576
Position (kb)
2723
2724
2725 Strain fitness (log2 ratio)
-1
0
1
2 at 2722.492 kb on + strand, within HP15_2574 at 2722.492 kb on - strand, within HP15_2574 at 2722.517 kb on + strand, within HP15_2574 at 2722.572 kb on - strand, within HP15_2574 at 2722.632 kb on + strand, within HP15_2574 at 2722.636 kb on + strand, within HP15_2574 at 2722.687 kb on + strand, within HP15_2574 at 2722.962 kb on + strand at 2722.990 kb on + strand at 2722.998 kb on - strand at 2723.003 kb on + strand at 2723.158 kb on - strand at 2723.191 kb on + strand at 2723.298 kb on - strand at 2723.367 kb on - strand at 2723.367 kb on - strand at 2723.386 kb on - strand, within HP15_2575 at 2723.404 kb on + strand, within HP15_2575 at 2723.412 kb on - strand, within HP15_2575 at 2723.446 kb on + strand, within HP15_2575 at 2723.454 kb on - strand, within HP15_2575 at 2723.541 kb on + strand, within HP15_2575 at 2723.712 kb on + strand, within HP15_2575 at 2723.720 kb on - strand, within HP15_2575 at 2723.720 kb on - strand, within HP15_2575 at 2723.727 kb on - strand, within HP15_2575 at 2723.738 kb on - strand, within HP15_2575 at 2723.768 kb on - strand, within HP15_2575 at 2723.840 kb on - strand, within HP15_2575 at 2723.869 kb on + strand, within HP15_2575 at 2723.872 kb on - strand, within HP15_2575 at 2723.909 kb on + strand, within HP15_2575 at 2723.917 kb on - strand, within HP15_2575 at 2723.917 kb on - strand, within HP15_2575 at 2723.997 kb on - strand, within HP15_2575 at 2724.165 kb on + strand at 2724.238 kb on - strand at 2724.257 kb on - strand, within HP15_2576 at 2724.264 kb on + strand, within HP15_2576 at 2724.272 kb on - strand, within HP15_2576 at 2724.274 kb on + strand, within HP15_2576 at 2724.282 kb on - strand, within HP15_2576 at 2724.315 kb on + strand, within HP15_2576 at 2724.315 kb on + strand, within HP15_2576 at 2724.526 kb on - strand, within HP15_2576 at 2724.582 kb on - strand, within HP15_2576 at 2724.638 kb on + strand, within HP15_2576 at 2724.668 kb on + strand, within HP15_2576 at 2724.704 kb on + strand, within HP15_2576 at 2724.732 kb on + strand, within HP15_2576 at 2724.896 kb on + strand, within HP15_2576 at 2724.902 kb on - strand, within HP15_2576 at 2724.925 kb on - strand, within HP15_2576 at 2724.948 kb on + strand, within HP15_2576 at 2725.060 kb on - strand, within HP15_2576 at 2725.078 kb on + strand, within HP15_2576
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 remove 2,722,492 + HP15_2574 0.55 +0.3 2,722,492 - HP15_2574 0.55 +0.2 2,722,517 + HP15_2574 0.57 -0.5 2,722,572 - HP15_2574 0.62 +1.5 2,722,632 + HP15_2574 0.66 -0.9 2,722,636 + HP15_2574 0.67 -0.0 2,722,687 + HP15_2574 0.71 +0.2 2,722,962 + -1.2 2,722,990 + -0.5 2,722,998 - +0.1 2,723,003 + -0.3 2,723,158 - +1.9 2,723,191 + -0.3 2,723,298 - +0.6 2,723,367 - -1.3 2,723,367 - -0.7 2,723,386 - HP15_2575 0.12 -0.0 2,723,404 + HP15_2575 0.14 -0.1 2,723,412 - HP15_2575 0.15 -0.8 2,723,446 + HP15_2575 0.20 -0.5 2,723,454 - HP15_2575 0.21 +0.2 2,723,541 + HP15_2575 0.32 -0.3 2,723,712 + HP15_2575 0.53 +0.2 2,723,720 - HP15_2575 0.54 +0.5 2,723,720 - HP15_2575 0.54 +0.5 2,723,727 - HP15_2575 0.55 +0.8 2,723,738 - HP15_2575 0.57 +0.1 2,723,768 - HP15_2575 0.61 -0.9 2,723,840 - HP15_2575 0.70 +0.9 2,723,869 + HP15_2575 0.73 -1.0 2,723,872 - HP15_2575 0.74 +0.1 2,723,909 + HP15_2575 0.78 -1.0 2,723,917 - HP15_2575 0.79 +0.4 2,723,917 - HP15_2575 0.79 +0.1 2,723,997 - HP15_2575 0.90 -0.1 2,724,165 + -0.1 2,724,238 - +0.7 2,724,257 - HP15_2576 0.10 +0.4 2,724,264 + HP15_2576 0.11 +1.3 2,724,272 - HP15_2576 0.11 -0.5 2,724,274 + HP15_2576 0.11 -0.0 2,724,282 - HP15_2576 0.12 +0.3 2,724,315 + HP15_2576 0.14 -0.0 2,724,315 + HP15_2576 0.14 +1.4 2,724,526 - HP15_2576 0.25 +0.5 2,724,582 - HP15_2576 0.28 -0.2 2,724,638 + HP15_2576 0.32 -1.3 2,724,668 + HP15_2576 0.33 +0.3 2,724,704 + HP15_2576 0.35 +0.6 2,724,732 + HP15_2576 0.37 +0.0 2,724,896 + HP15_2576 0.46 +0.3 2,724,902 - HP15_2576 0.46 -0.0 2,724,925 - HP15_2576 0.47 +0.2 2,724,948 + HP15_2576 0.49 -1.2 2,725,060 - HP15_2576 0.55 +1.4 2,725,078 + HP15_2576 0.56 +0.5
Or see this region's nucleotide sequence