Experiment: Tween 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1552 and HP15_1553 are separated by 28 nucleotides HP15_1553 and HP15_1554 are separated by 66 nucleotides HP15_1554 and HP15_tRNA30 are separated by 205 nucleotides HP15_tRNA30 and HP15_1555 are separated by 77 nucleotides
HP15_1552: HP15_1552 - conserved hypothetical protein, at 1,634,883 to 1,635,248
_1552
HP15_1553: HP15_1553 - transcription negative regulator, at 1,635,277 to 1,635,936
_1553
HP15_1554: HP15_1554 - hydrolase, isochorismatase family, at 1,636,003 to 1,636,533
_1554
HP15_tRNA30: HP15_tRNA30 - tRNA-Ser, at 1,636,739 to 1,636,829
_tRNA30
HP15_1555: HP15_1555 - protein containing DUF938, at 1,636,907 to 1,637,509
_1555
Position (kb)
1636
1637 Strain fitness (log2 ratio)
-2
-1
0
1 at 1635.176 kb on - strand, within HP15_1552 at 1635.250 kb on - strand at 1635.252 kb on + strand at 1635.260 kb on - strand at 1635.260 kb on - strand at 1635.260 kb on - strand at 1635.338 kb on + strand at 1635.357 kb on + strand, within HP15_1553 at 1635.357 kb on + strand, within HP15_1553 at 1635.365 kb on - strand, within HP15_1553 at 1635.796 kb on - strand, within HP15_1553 at 1635.806 kb on - strand, within HP15_1553 at 1635.943 kb on + strand at 1636.022 kb on - strand at 1636.091 kb on - strand, within HP15_1554 at 1636.205 kb on - strand, within HP15_1554 at 1636.380 kb on + strand, within HP15_1554 at 1636.388 kb on - strand, within HP15_1554 at 1636.441 kb on + strand, within HP15_1554 at 1636.887 kb on - strand at 1637.040 kb on - strand, within HP15_1555 at 1637.075 kb on + strand, within HP15_1555 at 1637.124 kb on + strand, within HP15_1555 at 1637.132 kb on - strand, within HP15_1555 at 1637.260 kb on - strand, within HP15_1555 at 1637.285 kb on - strand, within HP15_1555 at 1637.365 kb on - strand, within HP15_1555 at 1637.382 kb on - strand, within HP15_1555 at 1637.384 kb on + strand, within HP15_1555 at 1637.392 kb on - strand, within HP15_1555
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 remove 1,635,176 - HP15_1552 0.80 -0.3 1,635,250 - -0.8 1,635,252 + +0.0 1,635,260 - +0.7 1,635,260 - -0.3 1,635,260 - +0.3 1,635,338 + -0.3 1,635,357 + HP15_1553 0.12 -2.0 1,635,357 + HP15_1553 0.12 +0.4 1,635,365 - HP15_1553 0.13 +0.6 1,635,796 - HP15_1553 0.79 -0.3 1,635,806 - HP15_1553 0.80 -0.3 1,635,943 + +0.3 1,636,022 - -0.2 1,636,091 - HP15_1554 0.17 -0.4 1,636,205 - HP15_1554 0.38 -0.8 1,636,380 + HP15_1554 0.71 +0.2 1,636,388 - HP15_1554 0.73 -1.7 1,636,441 + HP15_1554 0.82 -1.1 1,636,887 - -0.3 1,637,040 - HP15_1555 0.22 +0.7 1,637,075 + HP15_1555 0.28 +0.2 1,637,124 + HP15_1555 0.36 -0.2 1,637,132 - HP15_1555 0.37 +0.6 1,637,260 - HP15_1555 0.59 +0.9 1,637,285 - HP15_1555 0.63 -1.8 1,637,365 - HP15_1555 0.76 +0.1 1,637,382 - HP15_1555 0.79 -0.8 1,637,384 + HP15_1555 0.79 -0.2 1,637,392 - HP15_1555 0.80 +0.7
Or see this region's nucleotide sequence