Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00015

Experiment: D-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00010 and BWI76_RS00015 are separated by 138 nucleotidesBWI76_RS00015 and BWI76_RS00020 are separated by 4 nucleotides BWI76_RS00010: BWI76_RS00010 - DNA replication and repair protein RecF, at 2,444 to 3,517 _RS00010 BWI76_RS00015: BWI76_RS00015 - DNA polymerase III subunit beta, at 3,656 to 4,756 _RS00015 BWI76_RS00020: BWI76_RS00020 - chromosomal replication initiation protein DnaA, at 4,761 to 6,161 _RS00020 Position (kb) 3 4 5Strain fitness (log2 ratio) -2 -1 0 1at 2.713 kb on + strand, within BWI76_RS00010at 2.831 kb on + strand, within BWI76_RS00010at 2.860 kb on + strand, within BWI76_RS00010at 2.933 kb on + strand, within BWI76_RS00010at 3.030 kb on + strand, within BWI76_RS00010at 3.120 kb on + strand, within BWI76_RS00010at 3.167 kb on + strand, within BWI76_RS00010at 3.256 kb on - strand, within BWI76_RS00010at 3.310 kb on + strand, within BWI76_RS00010at 3.318 kb on - strand, within BWI76_RS00010at 3.318 kb on - strand, within BWI76_RS00010at 3.318 kb on - strand, within BWI76_RS00010at 3.318 kb on - strand, within BWI76_RS00010at 3.318 kb on - strand, within BWI76_RS00010at 3.529 kb on + strandat 3.561 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (N)
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2,713 + BWI76_RS00010 0.25 -0.3
2,831 + BWI76_RS00010 0.36 -0.4
2,860 + BWI76_RS00010 0.39 -1.9
2,933 + BWI76_RS00010 0.46 -0.4
3,030 + BWI76_RS00010 0.55 +0.6
3,120 + BWI76_RS00010 0.63 +0.4
3,167 + BWI76_RS00010 0.67 -0.6
3,256 - BWI76_RS00010 0.76 +0.2
3,310 + BWI76_RS00010 0.81 +0.4
3,318 - BWI76_RS00010 0.81 -0.9
3,318 - BWI76_RS00010 0.81 -1.4
3,318 - BWI76_RS00010 0.81 -0.2
3,318 - BWI76_RS00010 0.81 +0.4
3,318 - BWI76_RS00010 0.81 -2.0
3,529 + +0.6
3,561 - -1.2

Or see this region's nucleotide sequence