Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1786 and Dshi_R0033 are separated by 164 nucleotides Dshi_R0033 and Dshi_1787 are separated by 52 nucleotides Dshi_1787 and Dshi_1788 overlap by 4 nucleotides Dshi_1788 and Dshi_1789 are separated by 132 nucleotides Dshi_1789 and Dshi_1790 are separated by 104 nucleotides
Dshi_1786: Dshi_1786 - hypothetical protein (RefSeq), at 1,858,657 to 1,859,097
_1786
Dshi_R0033: Dshi_R0033 - tRNA-Pro (RefSeq), at 1,859,262 to 1,859,338
_R0033
Dshi_1787: Dshi_1787 - protein of unknown function DUF192 (RefSeq), at 1,859,391 to 1,859,876
_1787
Dshi_1788: Dshi_1788 - cold-shock DNA-binding domain protein (RefSeq), at 1,859,873 to 1,860,394
_1788
Dshi_1789: Dshi_1789 - Pyridoxal 5'-phosphate synthase (RefSeq), at 1,860,527 to 1,861,132
_1789
Dshi_1790: Dshi_1790 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,861,237 to 1,862,055
_1790
Position (kb)
1859
1860
1861 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1858.883 kb on + strand, within Dshi_1786 at 1858.883 kb on + strand, within Dshi_1786 at 1859.070 kb on + strand at 1859.115 kb on - strand at 1860.050 kb on + strand, within Dshi_1788 at 1860.086 kb on + strand, within Dshi_1788 at 1860.100 kb on - strand, within Dshi_1788 at 1860.210 kb on - strand, within Dshi_1788 at 1860.313 kb on + strand, within Dshi_1788 at 1860.321 kb on - strand, within Dshi_1788 at 1860.321 kb on - strand, within Dshi_1788 at 1860.321 kb on - strand, within Dshi_1788 at 1860.344 kb on + strand at 1860.352 kb on - strand at 1860.354 kb on + strand at 1860.356 kb on + strand at 1860.356 kb on + strand at 1860.378 kb on - strand at 1860.380 kb on - strand at 1860.406 kb on - strand at 1860.420 kb on - strand at 1860.444 kb on + strand at 1860.453 kb on + strand at 1860.453 kb on + strand at 1860.461 kb on - strand at 1860.474 kb on + strand at 1860.486 kb on + strand at 1860.507 kb on + strand at 1861.149 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 2 mM remove 1,858,883 + Dshi_1786 0.51 -0.4 1,858,883 + Dshi_1786 0.51 -0.9 1,859,070 + -0.4 1,859,115 - +0.2 1,860,050 + Dshi_1788 0.34 -1.1 1,860,086 + Dshi_1788 0.41 -1.4 1,860,100 - Dshi_1788 0.43 +0.1 1,860,210 - Dshi_1788 0.65 -0.8 1,860,313 + Dshi_1788 0.84 -0.0 1,860,321 - Dshi_1788 0.86 -0.8 1,860,321 - Dshi_1788 0.86 -3.1 1,860,321 - Dshi_1788 0.86 -0.7 1,860,344 + +1.0 1,860,352 - -1.1 1,860,354 + -1.1 1,860,356 + -2.1 1,860,356 + -2.1 1,860,378 - -0.8 1,860,380 - -0.5 1,860,406 - -1.6 1,860,420 - -0.2 1,860,444 + -1.8 1,860,453 + -1.9 1,860,453 + +1.4 1,860,461 - -1.6 1,860,474 + +0.0 1,860,486 + -1.5 1,860,507 + -1.7 1,861,149 - -0.9
Or see this region's nucleotide sequence