Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_681

Experiment: sodium carbonate 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_680 and Pf6N2E2_681 overlap by 4 nucleotidesPf6N2E2_681 and Pf6N2E2_682 are separated by 112 nucleotidesPf6N2E2_682 and Pf6N2E2_683 are separated by 203 nucleotides Pf6N2E2_680 - D-amino-acid oxidase (EC 1.4.3.3), at 784,720 to 785,835 _680 Pf6N2E2_681 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 785,832 to 786,764 _681 Pf6N2E2_682 - Predicted regulator PutR for proline utilization, GntR family, at 786,877 to 787,548 _682 Pf6N2E2_683 - Probable MFS transporter, at 787,752 to 788,918 _683 Position (kb) 785 786 787Strain fitness (log2 ratio) -2 -1 0 1 2at 785.092 kb on + strand, within Pf6N2E2_680at 785.092 kb on + strand, within Pf6N2E2_680at 785.092 kb on + strand, within Pf6N2E2_680at 785.092 kb on + strand, within Pf6N2E2_680at 785.092 kb on + strand, within Pf6N2E2_680at 785.093 kb on - strand, within Pf6N2E2_680at 785.093 kb on - strand, within Pf6N2E2_680at 785.093 kb on - strand, within Pf6N2E2_680at 785.093 kb on - strand, within Pf6N2E2_680at 785.093 kb on - strand, within Pf6N2E2_680at 785.093 kb on - strand, within Pf6N2E2_680at 785.093 kb on - strand, within Pf6N2E2_680at 785.094 kb on + strand, within Pf6N2E2_680at 785.094 kb on + strand, within Pf6N2E2_680at 785.095 kb on - strand, within Pf6N2E2_680at 785.095 kb on - strand, within Pf6N2E2_680at 785.142 kb on - strand, within Pf6N2E2_680at 785.142 kb on - strand, within Pf6N2E2_680at 785.397 kb on - strand, within Pf6N2E2_680at 785.522 kb on + strand, within Pf6N2E2_680at 785.522 kb on + strand, within Pf6N2E2_680at 785.523 kb on - strand, within Pf6N2E2_680at 785.947 kb on + strand, within Pf6N2E2_681at 785.947 kb on + strand, within Pf6N2E2_681at 786.073 kb on + strand, within Pf6N2E2_681at 786.074 kb on - strand, within Pf6N2E2_681at 786.093 kb on + strand, within Pf6N2E2_681at 786.093 kb on + strand, within Pf6N2E2_681at 786.198 kb on + strand, within Pf6N2E2_681at 786.206 kb on - strand, within Pf6N2E2_681at 786.206 kb on - strand, within Pf6N2E2_681at 786.206 kb on - strand, within Pf6N2E2_681at 786.211 kb on + strand, within Pf6N2E2_681at 786.355 kb on + strand, within Pf6N2E2_681at 786.355 kb on + strand, within Pf6N2E2_681at 786.408 kb on + strand, within Pf6N2E2_681at 786.832 kb on - strandat 786.832 kb on - strandat 786.837 kb on + strandat 786.837 kb on + strandat 786.838 kb on - strandat 786.838 kb on - strandat 786.838 kb on - strandat 787.115 kb on + strand, within Pf6N2E2_682at 787.116 kb on - strand, within Pf6N2E2_682at 787.116 kb on - strand, within Pf6N2E2_682at 787.116 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.246 kb on + strand, within Pf6N2E2_682at 787.247 kb on - strand, within Pf6N2E2_682at 787.585 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium carbonate 0.0125 M
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785,092 + Pf6N2E2_680 0.33 -0.2
785,092 + Pf6N2E2_680 0.33 -0.6
785,092 + Pf6N2E2_680 0.33 +0.0
785,092 + Pf6N2E2_680 0.33 +0.8
785,092 + Pf6N2E2_680 0.33 -0.6
785,093 - Pf6N2E2_680 0.33 -2.5
785,093 - Pf6N2E2_680 0.33 +0.5
785,093 - Pf6N2E2_680 0.33 +0.4
785,093 - Pf6N2E2_680 0.33 +0.4
785,093 - Pf6N2E2_680 0.33 +0.2
785,093 - Pf6N2E2_680 0.33 -0.5
785,093 - Pf6N2E2_680 0.33 -0.4
785,094 + Pf6N2E2_680 0.34 +0.3
785,094 + Pf6N2E2_680 0.34 -1.5
785,095 - Pf6N2E2_680 0.34 +1.9
785,095 - Pf6N2E2_680 0.34 +1.1
785,142 - Pf6N2E2_680 0.38 +0.6
785,142 - Pf6N2E2_680 0.38 -1.6
785,397 - Pf6N2E2_680 0.61 +0.5
785,522 + Pf6N2E2_680 0.72 +0.6
785,522 + Pf6N2E2_680 0.72 +1.2
785,523 - Pf6N2E2_680 0.72 +1.2
785,947 + Pf6N2E2_681 0.12 +2.4
785,947 + Pf6N2E2_681 0.12 +1.4
786,073 + Pf6N2E2_681 0.26 +1.2
786,074 - Pf6N2E2_681 0.26 +0.5
786,093 + Pf6N2E2_681 0.28 +0.0
786,093 + Pf6N2E2_681 0.28 +0.6
786,198 + Pf6N2E2_681 0.39 +0.9
786,206 - Pf6N2E2_681 0.40 -0.5
786,206 - Pf6N2E2_681 0.40 +1.2
786,206 - Pf6N2E2_681 0.40 +0.0
786,211 + Pf6N2E2_681 0.41 +0.8
786,355 + Pf6N2E2_681 0.56 -0.4
786,355 + Pf6N2E2_681 0.56 +0.8
786,408 + Pf6N2E2_681 0.62 +1.4
786,832 - -0.2
786,832 - +0.6
786,837 + -0.4
786,837 + +0.7
786,838 - -1.2
786,838 - +0.3
786,838 - +0.2
787,115 + Pf6N2E2_682 0.35 -0.5
787,116 - Pf6N2E2_682 0.36 -0.7
787,116 - Pf6N2E2_682 0.36 +0.8
787,116 - Pf6N2E2_682 0.36 +1.0
787,160 - Pf6N2E2_682 0.42 +0.8
787,160 - Pf6N2E2_682 0.42 -0.4
787,160 - Pf6N2E2_682 0.42 +1.6
787,160 - Pf6N2E2_682 0.42 +0.5
787,246 + Pf6N2E2_682 0.55 -0.5
787,247 - Pf6N2E2_682 0.55 -0.7
787,585 + +1.0

Or see this region's nucleotide sequence