Experiment: sodium carbonate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3196 and Pf6N2E2_3197 are separated by 63 nucleotides Pf6N2E2_3197 and Pf6N2E2_3198 are separated by 43 nucleotides Pf6N2E2_3198 and Pf6N2E2_3199 overlap by 4 nucleotides
Pf6N2E2_3196 - Protein-export membrane protein SecD (TC 3.A.5.1.1), at 3,689,260 to 3,691,128
_3196
Pf6N2E2_3197 - Preprotein translocase subunit YajC (TC 3.A.5.1.1), at 3,691,192 to 3,691,530
_3197
Pf6N2E2_3198 - tRNA-guanine transglycosylase (EC 2.4.2.29), at 3,691,574 to 3,692,707
_3198
Pf6N2E2_3199 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 3,692,704 to 3,693,753
_3199
Position (kb)
3691
3692
3693 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3691.180 kb on - strand at 3691.180 kb on - strand at 3691.190 kb on - strand at 3691.190 kb on - strand at 3691.194 kb on - strand at 3691.194 kb on - strand at 3691.194 kb on - strand at 3691.194 kb on - strand at 3691.194 kb on - strand at 3691.194 kb on - strand at 3691.551 kb on - strand at 3691.612 kb on - strand at 3691.640 kb on - strand at 3691.673 kb on - strand at 3691.706 kb on - strand, within Pf6N2E2_3198 at 3691.715 kb on - strand, within Pf6N2E2_3198 at 3692.587 kb on - strand, within Pf6N2E2_3198 at 3692.708 kb on - strand at 3692.781 kb on - strand at 3692.804 kb on + strand at 3692.804 kb on + strand at 3692.805 kb on - strand at 3692.805 kb on - strand at 3692.805 kb on - strand at 3692.926 kb on - strand, within Pf6N2E2_3199 at 3693.261 kb on - strand, within Pf6N2E2_3199 at 3693.307 kb on + strand, within Pf6N2E2_3199 at 3693.308 kb on - strand, within Pf6N2E2_3199 at 3693.309 kb on + strand, within Pf6N2E2_3199 at 3693.309 kb on + strand, within Pf6N2E2_3199 at 3693.309 kb on + strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.310 kb on - strand, within Pf6N2E2_3199 at 3693.312 kb on - strand, within Pf6N2E2_3199 at 3693.312 kb on - strand, within Pf6N2E2_3199
Per-strain Table
Position Strand Gene LocusTag Fraction sodium carbonate 0.0125 M remove 3,691,180 - -0.3 3,691,180 - -0.4 3,691,190 - +1.1 3,691,190 - -1.3 3,691,194 - +1.5 3,691,194 - +0.6 3,691,194 - -0.7 3,691,194 - -0.8 3,691,194 - -0.7 3,691,194 - -0.3 3,691,551 - -0.2 3,691,612 - -0.4 3,691,640 - -1.7 3,691,673 - -0.4 3,691,706 - Pf6N2E2_3198 0.12 -1.0 3,691,715 - Pf6N2E2_3198 0.12 -0.8 3,692,587 - Pf6N2E2_3198 0.89 -2.3 3,692,708 - +1.1 3,692,781 - -0.2 3,692,804 + +0.6 3,692,804 + +1.8 3,692,805 - +0.5 3,692,805 - -0.5 3,692,805 - -1.0 3,692,926 - Pf6N2E2_3199 0.21 -0.5 3,693,261 - Pf6N2E2_3199 0.53 +1.0 3,693,307 + Pf6N2E2_3199 0.57 -1.5 3,693,308 - Pf6N2E2_3199 0.58 +2.2 3,693,309 + Pf6N2E2_3199 0.58 +1.1 3,693,309 + Pf6N2E2_3199 0.58 -1.2 3,693,309 + Pf6N2E2_3199 0.58 -0.2 3,693,310 - Pf6N2E2_3199 0.58 +0.5 3,693,310 - Pf6N2E2_3199 0.58 -0.8 3,693,310 - Pf6N2E2_3199 0.58 -0.8 3,693,310 - Pf6N2E2_3199 0.58 -0.2 3,693,310 - Pf6N2E2_3199 0.58 +1.5 3,693,310 - Pf6N2E2_3199 0.58 +0.7 3,693,310 - Pf6N2E2_3199 0.58 -0.4 3,693,310 - Pf6N2E2_3199 0.58 +0.5 3,693,310 - Pf6N2E2_3199 0.58 -0.3 3,693,310 - Pf6N2E2_3199 0.58 -1.0 3,693,310 - Pf6N2E2_3199 0.58 -1.1 3,693,310 - Pf6N2E2_3199 0.58 -1.2 3,693,312 - Pf6N2E2_3199 0.58 -0.0 3,693,312 - Pf6N2E2_3199 0.58 +0.4
Or see this region's nucleotide sequence