Experiment: sodium carbonate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2875 and Pf6N2E2_2876 are separated by 44 nucleotides Pf6N2E2_2876 and Pf6N2E2_2877 are separated by 160 nucleotides Pf6N2E2_2877 and Pf6N2E2_2878 are separated by 9 nucleotides Pf6N2E2_2878 and Pf6N2E2_2879 overlap by 14 nucleotides
Pf6N2E2_2875 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative, at 3,363,617 to 3,364,561
_2875
Pf6N2E2_2876 - enoyl-CoA hydratase, R-specific, at 3,364,606 to 3,365,076
_2876
Pf6N2E2_2877 - Membrane fusion component of tripartite multidrug resistance system, at 3,365,237 to 3,366,100
_2877
Pf6N2E2_2878 - required for 4-hydroxybenzoate and octanoate transport, together with MFP and FUSC proteins (Pf6N2E2_2877, Pf6N2E2_2879) (from data) , at 3,366,110 to 3,366,316
_2878
Pf6N2E2_2879 - membrane protein, putative, at 3,366,303 to 3,368,303
_2879
Position (kb)
3365
3366
3367 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3364.582 kb on - strand at 3365.131 kb on + strand at 3365.239 kb on - strand at 3365.239 kb on - strand at 3365.239 kb on - strand at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.538 kb on + strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.539 kb on - strand, within Pf6N2E2_2877 at 3365.589 kb on + strand, within Pf6N2E2_2877 at 3365.589 kb on + strand, within Pf6N2E2_2877 at 3365.590 kb on - strand, within Pf6N2E2_2877 at 3365.590 kb on - strand, within Pf6N2E2_2877 at 3365.590 kb on - strand, within Pf6N2E2_2877 at 3365.590 kb on - strand, within Pf6N2E2_2877 at 3366.018 kb on + strand at 3366.018 kb on + strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.019 kb on - strand at 3366.022 kb on - strand at 3366.086 kb on + strand at 3366.087 kb on - strand at 3366.094 kb on + strand at 3366.094 kb on + strand at 3366.094 kb on + strand at 3366.094 kb on + strand at 3366.094 kb on + strand at 3366.094 kb on + strand at 3366.094 kb on + strand at 3366.094 kb on + strand at 3366.095 kb on - strand at 3366.095 kb on - strand at 3366.095 kb on - strand at 3366.095 kb on - strand at 3366.095 kb on - strand at 3366.095 kb on - strand at 3366.095 kb on - strand at 3366.095 kb on - strand at 3366.111 kb on + strand at 3366.111 kb on + strand at 3366.112 kb on - strand at 3366.134 kb on - strand, within Pf6N2E2_2878 at 3366.137 kb on - strand, within Pf6N2E2_2878 at 3366.156 kb on + strand, within Pf6N2E2_2878 at 3366.156 kb on + strand, within Pf6N2E2_2878 at 3366.156 kb on + strand, within Pf6N2E2_2878 at 3366.261 kb on + strand, within Pf6N2E2_2878 at 3366.262 kb on - strand, within Pf6N2E2_2878 at 3366.262 kb on - strand, within Pf6N2E2_2878 at 3366.661 kb on + strand, within Pf6N2E2_2879 at 3366.889 kb on + strand, within Pf6N2E2_2879 at 3366.889 kb on + strand, within Pf6N2E2_2879 at 3366.889 kb on + strand, within Pf6N2E2_2879 at 3366.890 kb on - strand, within Pf6N2E2_2879 at 3366.958 kb on - strand, within Pf6N2E2_2879 at 3367.048 kb on - strand, within Pf6N2E2_2879 at 3367.048 kb on - strand, within Pf6N2E2_2879
Per-strain Table
Position Strand Gene LocusTag Fraction sodium carbonate 0.0125 M remove 3,364,582 - +1.6 3,365,131 + +0.5 3,365,239 - +0.3 3,365,239 - +2.0 3,365,239 - -0.2 3,365,538 + Pf6N2E2_2877 0.35 -0.6 3,365,538 + Pf6N2E2_2877 0.35 -0.2 3,365,538 + Pf6N2E2_2877 0.35 -0.2 3,365,538 + Pf6N2E2_2877 0.35 +0.1 3,365,538 + Pf6N2E2_2877 0.35 +0.7 3,365,538 + Pf6N2E2_2877 0.35 -0.1 3,365,538 + Pf6N2E2_2877 0.35 +0.4 3,365,538 + Pf6N2E2_2877 0.35 -0.9 3,365,539 - Pf6N2E2_2877 0.35 -1.9 3,365,539 - Pf6N2E2_2877 0.35 +0.1 3,365,539 - Pf6N2E2_2877 0.35 -1.2 3,365,539 - Pf6N2E2_2877 0.35 -1.2 3,365,539 - Pf6N2E2_2877 0.35 -1.5 3,365,539 - Pf6N2E2_2877 0.35 -0.1 3,365,539 - Pf6N2E2_2877 0.35 -0.1 3,365,539 - Pf6N2E2_2877 0.35 -1.2 3,365,589 + Pf6N2E2_2877 0.41 -1.6 3,365,589 + Pf6N2E2_2877 0.41 -0.6 3,365,590 - Pf6N2E2_2877 0.41 -0.8 3,365,590 - Pf6N2E2_2877 0.41 +0.7 3,365,590 - Pf6N2E2_2877 0.41 +0.0 3,365,590 - Pf6N2E2_2877 0.41 +3.2 3,366,018 + +0.1 3,366,018 + -0.1 3,366,019 - +0.3 3,366,019 - -0.3 3,366,019 - -0.0 3,366,019 - -1.2 3,366,019 - -0.4 3,366,019 - +0.5 3,366,019 - -1.5 3,366,019 - +0.4 3,366,019 - -0.5 3,366,019 - -0.7 3,366,019 - -0.2 3,366,022 - +0.2 3,366,086 + -1.5 3,366,087 - +1.3 3,366,094 + +1.0 3,366,094 + +0.8 3,366,094 + +1.2 3,366,094 + -1.8 3,366,094 + +0.5 3,366,094 + -0.7 3,366,094 + -0.1 3,366,094 + +0.9 3,366,095 - -0.7 3,366,095 - -0.6 3,366,095 - +1.1 3,366,095 - -0.2 3,366,095 - -1.0 3,366,095 - -0.0 3,366,095 - -0.0 3,366,095 - -0.2 3,366,111 + +0.7 3,366,111 + -0.6 3,366,112 - +0.2 3,366,134 - Pf6N2E2_2878 0.12 -0.8 3,366,137 - Pf6N2E2_2878 0.13 +0.3 3,366,156 + Pf6N2E2_2878 0.22 +0.4 3,366,156 + Pf6N2E2_2878 0.22 +0.8 3,366,156 + Pf6N2E2_2878 0.22 -0.2 3,366,261 + Pf6N2E2_2878 0.73 -0.8 3,366,262 - Pf6N2E2_2878 0.73 +0.6 3,366,262 - Pf6N2E2_2878 0.73 -1.2 3,366,661 + Pf6N2E2_2879 0.18 -0.2 3,366,889 + Pf6N2E2_2879 0.29 +0.2 3,366,889 + Pf6N2E2_2879 0.29 -1.5 3,366,889 + Pf6N2E2_2879 0.29 -0.6 3,366,890 - Pf6N2E2_2879 0.29 +0.2 3,366,958 - Pf6N2E2_2879 0.33 +0.3 3,367,048 - Pf6N2E2_2879 0.37 +0.4 3,367,048 - Pf6N2E2_2879 0.37 -0.6
Or see this region's nucleotide sequence