Experiment: Glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_802 and HP15_801 are separated by 141 nucleotides HP15_801 and HP15_803 are separated by 26 nucleotides HP15_803 and HP15_804 overlap by 23 nucleotides
HP15_802: HP15_802 - glycerol-3-phosphate acyltransferase, at 815,381 to 817,849
_802
HP15_801: HP15_801 - NADH pyrophosphatase, at 817,991 to 818,818
_801
HP15_803: HP15_803 - glutamate racemase, at 818,845 to 819,678
_803
HP15_804: HP15_804 - protein containing DUF470, at 819,656 to 820,663
_804
Position (kb) 817
818
819 Strain fitness (log2 ratio) -1
0
1 at 817.011 kb on + strand, within HP15_802 at 817.019 kb on + strand, within HP15_802 at 817.174 kb on - strand, within HP15_802 at 817.178 kb on + strand, within HP15_802 at 817.196 kb on + strand, within HP15_802 at 817.260 kb on + strand, within HP15_802 at 817.268 kb on - strand, within HP15_802 at 817.469 kb on + strand, within HP15_802 at 817.651 kb on + strand at 817.678 kb on + strand at 817.730 kb on + strand at 817.779 kb on - strand at 817.900 kb on - strand at 818.208 kb on - strand, within HP15_801 at 818.567 kb on + strand, within HP15_801 at 819.612 kb on + strand at 819.646 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol remove 817,011 + HP15_802 0.66 +0.3 817,019 + HP15_802 0.66 -1.0 817,174 - HP15_802 0.73 -0.5 817,178 + HP15_802 0.73 +0.5 817,196 + HP15_802 0.74 +0.0 817,260 + HP15_802 0.76 -0.2 817,268 - HP15_802 0.76 +0.6 817,469 + HP15_802 0.85 +0.4 817,651 + -0.2 817,678 + +0.1 817,730 + -0.0 817,779 - -0.0 817,900 - +0.3 818,208 - HP15_801 0.26 -1.4 818,567 + HP15_801 0.70 -0.3 819,612 + -0.1 819,646 + +0.7
Or see this region's nucleotide sequence