Experiment: Glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3926 and HP15_3927 are separated by 20 nucleotides HP15_3927 and HP15_3928 are separated by 341 nucleotides HP15_3928 and HP15_3929 are separated by 10 nucleotides
HP15_3926: HP15_3926 - acyl-CoA synthetase, at 4,155,110 to 4,156,774
_3926
HP15_3927: HP15_3927 - protein containing helix-turn-helix type 3 domain, at 4,156,795 to 4,157,160
_3927
HP15_3928: HP15_3928 - acyl-CoA dehydrogenase domain protein, at 4,157,502 to 4,158,638
_3928
HP15_3929: HP15_3929 - acyl-CoA dehydrogenase domain protein, at 4,158,649 to 4,159,845
_3929
Position (kb)
4157
4158
4159 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4156.503 kb on + strand, within HP15_3926 at 4156.600 kb on - strand, within HP15_3926 at 4156.633 kb on + strand at 4156.665 kb on + strand at 4156.945 kb on - strand, within HP15_3927 at 4157.070 kb on + strand, within HP15_3927 at 4157.078 kb on - strand, within HP15_3927 at 4157.078 kb on - strand, within HP15_3927 at 4157.087 kb on + strand, within HP15_3927 at 4157.087 kb on + strand, within HP15_3927 at 4157.090 kb on - strand, within HP15_3927 at 4157.097 kb on + strand, within HP15_3927 at 4157.353 kb on + strand at 4157.413 kb on + strand at 4157.439 kb on + strand at 4157.478 kb on - strand at 4157.518 kb on + strand at 4157.518 kb on + strand at 4157.539 kb on - strand at 4157.603 kb on - strand at 4157.603 kb on - strand at 4157.637 kb on + strand, within HP15_3928 at 4157.687 kb on - strand, within HP15_3928 at 4157.854 kb on - strand, within HP15_3928 at 4157.856 kb on + strand, within HP15_3928 at 4157.863 kb on - strand, within HP15_3928 at 4157.868 kb on + strand, within HP15_3928 at 4157.874 kb on + strand, within HP15_3928 at 4157.882 kb on - strand, within HP15_3928 at 4157.887 kb on - strand, within HP15_3928 at 4157.889 kb on + strand, within HP15_3928 at 4157.952 kb on + strand, within HP15_3928 at 4157.975 kb on + strand, within HP15_3928 at 4157.983 kb on + strand, within HP15_3928 at 4157.991 kb on - strand, within HP15_3928 at 4158.046 kb on + strand, within HP15_3928 at 4158.046 kb on + strand, within HP15_3928 at 4158.069 kb on - strand, within HP15_3928 at 4158.186 kb on + strand, within HP15_3928 at 4158.431 kb on - strand, within HP15_3928 at 4158.488 kb on - strand, within HP15_3928 at 4158.493 kb on - strand, within HP15_3928 at 4158.540 kb on + strand at 4158.557 kb on + strand at 4158.565 kb on - strand at 4158.597 kb on + strand at 4158.648 kb on + strand at 4158.787 kb on + strand, within HP15_3929 at 4158.844 kb on + strand, within HP15_3929 at 4158.975 kb on - strand, within HP15_3929 at 4159.007 kb on - strand, within HP15_3929 at 4159.021 kb on - strand, within HP15_3929 at 4159.023 kb on + strand, within HP15_3929 at 4159.090 kb on + strand, within HP15_3929 at 4159.141 kb on + strand, within HP15_3929 at 4159.141 kb on + strand, within HP15_3929 at 4159.142 kb on + strand, within HP15_3929 at 4159.149 kb on - strand, within HP15_3929 at 4159.150 kb on - strand, within HP15_3929 at 4159.179 kb on - strand, within HP15_3929 at 4159.228 kb on + strand, within HP15_3929 at 4159.257 kb on + strand, within HP15_3929 at 4159.356 kb on + strand, within HP15_3929 at 4159.364 kb on - strand, within HP15_3929 at 4159.415 kb on + strand, within HP15_3929 at 4159.437 kb on + strand, within HP15_3929 at 4159.437 kb on + strand, within HP15_3929 at 4159.437 kb on + strand, within HP15_3929 at 4159.445 kb on - strand, within HP15_3929 at 4159.445 kb on - strand, within HP15_3929 at 4159.445 kb on - strand, within HP15_3929 at 4159.445 kb on - strand, within HP15_3929 at 4159.445 kb on - strand, within HP15_3929 at 4159.460 kb on + strand, within HP15_3929 at 4159.463 kb on - strand, within HP15_3929 at 4159.470 kb on + strand, within HP15_3929 at 4159.482 kb on - strand, within HP15_3929 at 4159.613 kb on + strand, within HP15_3929
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol remove 4,156,503 + HP15_3926 0.84 +0.4 4,156,600 - HP15_3926 0.89 -0.4 4,156,633 + +0.1 4,156,665 + +0.3 4,156,945 - HP15_3927 0.41 +0.1 4,157,070 + HP15_3927 0.75 +0.2 4,157,078 - HP15_3927 0.77 +0.6 4,157,078 - HP15_3927 0.77 +0.7 4,157,087 + HP15_3927 0.80 -0.1 4,157,087 + HP15_3927 0.80 -0.7 4,157,090 - HP15_3927 0.81 -1.4 4,157,097 + HP15_3927 0.83 -0.4 4,157,353 + -0.1 4,157,413 + -0.8 4,157,439 + -0.7 4,157,478 - +0.8 4,157,518 + -0.4 4,157,518 + +0.4 4,157,539 - +0.6 4,157,603 - -0.6 4,157,603 - -1.7 4,157,637 + HP15_3928 0.12 -1.0 4,157,687 - HP15_3928 0.16 +0.8 4,157,854 - HP15_3928 0.31 -0.4 4,157,856 + HP15_3928 0.31 +0.1 4,157,863 - HP15_3928 0.32 -2.4 4,157,868 + HP15_3928 0.32 -0.6 4,157,874 + HP15_3928 0.33 +1.6 4,157,882 - HP15_3928 0.33 -0.6 4,157,887 - HP15_3928 0.34 +1.0 4,157,889 + HP15_3928 0.34 +0.6 4,157,952 + HP15_3928 0.40 +0.2 4,157,975 + HP15_3928 0.42 -1.4 4,157,983 + HP15_3928 0.42 -0.6 4,157,991 - HP15_3928 0.43 -2.2 4,158,046 + HP15_3928 0.48 -1.1 4,158,046 + HP15_3928 0.48 -0.9 4,158,069 - HP15_3928 0.50 -0.4 4,158,186 + HP15_3928 0.60 +0.1 4,158,431 - HP15_3928 0.82 -0.4 4,158,488 - HP15_3928 0.87 -1.1 4,158,493 - HP15_3928 0.87 -1.2 4,158,540 + -0.5 4,158,557 + -0.8 4,158,565 - +0.1 4,158,597 + -0.4 4,158,648 + +0.6 4,158,787 + HP15_3929 0.12 -1.9 4,158,844 + HP15_3929 0.16 -1.5 4,158,975 - HP15_3929 0.27 -1.7 4,159,007 - HP15_3929 0.30 -0.2 4,159,021 - HP15_3929 0.31 +0.0 4,159,023 + HP15_3929 0.31 +0.4 4,159,090 + HP15_3929 0.37 -1.1 4,159,141 + HP15_3929 0.41 -1.4 4,159,141 + HP15_3929 0.41 -1.9 4,159,142 + HP15_3929 0.41 -0.8 4,159,149 - HP15_3929 0.42 -0.8 4,159,150 - HP15_3929 0.42 +0.2 4,159,179 - HP15_3929 0.44 -0.5 4,159,228 + HP15_3929 0.48 -0.5 4,159,257 + HP15_3929 0.51 -0.7 4,159,356 + HP15_3929 0.59 -3.2 4,159,364 - HP15_3929 0.60 -1.4 4,159,415 + HP15_3929 0.64 -0.4 4,159,437 + HP15_3929 0.66 -1.0 4,159,437 + HP15_3929 0.66 -1.9 4,159,437 + HP15_3929 0.66 -1.7 4,159,445 - HP15_3929 0.66 +0.6 4,159,445 - HP15_3929 0.66 -0.9 4,159,445 - HP15_3929 0.66 -1.8 4,159,445 - HP15_3929 0.66 -0.7 4,159,445 - HP15_3929 0.66 -0.2 4,159,460 + HP15_3929 0.68 +0.9 4,159,463 - HP15_3929 0.68 -0.4 4,159,470 + HP15_3929 0.69 -1.0 4,159,482 - HP15_3929 0.70 -1.2 4,159,613 + HP15_3929 0.81 -1.3
Or see this region's nucleotide sequence