Experiment: Glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_35 and HP15_36 are separated by 2 nucleotides HP15_36 and HP15_37 are separated by 382 nucleotides HP15_37 and HP15_38 are separated by 26 nucleotides
HP15_35: HP15_35 - thiolase, at 39,616 to 40,806
_35
HP15_36: HP15_36 - protein containing DUF35, at 40,809 to 40,964
_36
HP15_37: HP15_37 - 3-ketoacyl-(acyl-carrier-protein) reductase, at 41,347 to 42,048
_37
HP15_38: HP15_38 - fumarylacetoacetate (FAA) hydrolase, at 42,075 to 43,064
_38
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 40.353 kb on - strand, within HP15_35 at 40.407 kb on + strand, within HP15_35 at 40.415 kb on - strand, within HP15_35 at 40.443 kb on + strand, within HP15_35 at 40.537 kb on + strand, within HP15_35 at 40.537 kb on + strand, within HP15_35 at 40.577 kb on + strand, within HP15_35 at 40.577 kb on - strand, within HP15_35 at 40.584 kb on + strand, within HP15_35 at 40.587 kb on - strand, within HP15_35 at 40.677 kb on - strand, within HP15_35 at 40.771 kb on - strand at 40.815 kb on - strand at 40.832 kb on + strand, within HP15_36 at 40.903 kb on + strand, within HP15_36 at 40.987 kb on - strand at 40.987 kb on - strand at 40.987 kb on - strand at 40.995 kb on - strand at 41.084 kb on + strand at 41.115 kb on - strand at 41.322 kb on + strand at 41.535 kb on - strand, within HP15_37 at 41.551 kb on + strand, within HP15_37 at 41.626 kb on - strand, within HP15_37 at 41.645 kb on - strand, within HP15_37 at 41.712 kb on + strand, within HP15_37 at 41.752 kb on - strand, within HP15_37 at 41.779 kb on + strand, within HP15_37 at 41.779 kb on + strand, within HP15_37 at 41.811 kb on + strand, within HP15_37 at 41.928 kb on - strand, within HP15_37 at 41.938 kb on - strand, within HP15_37 at 42.021 kb on + strand at 42.043 kb on + strand at 42.051 kb on - strand at 42.088 kb on + strand at 42.131 kb on - strand at 42.149 kb on + strand at 42.156 kb on - strand at 42.164 kb on + strand at 42.164 kb on + strand at 42.230 kb on + strand, within HP15_38 at 42.230 kb on + strand, within HP15_38 at 42.238 kb on - strand, within HP15_38 at 42.314 kb on - strand, within HP15_38 at 42.375 kb on + strand, within HP15_38 at 42.403 kb on + strand, within HP15_38 at 42.415 kb on + strand, within HP15_38 at 42.426 kb on - strand, within HP15_38 at 42.688 kb on + strand, within HP15_38 at 42.749 kb on - strand, within HP15_38 at 42.769 kb on + strand, within HP15_38 at 42.840 kb on + strand, within HP15_38 at 42.887 kb on + strand, within HP15_38 at 42.915 kb on + strand, within HP15_38 at 42.915 kb on + strand, within HP15_38 at 42.923 kb on - strand, within HP15_38 at 42.949 kb on + strand, within HP15_38 at 42.957 kb on - strand, within HP15_38 at 43.025 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol remove 40,353 - HP15_35 0.62 -0.2 40,407 + HP15_35 0.66 -0.2 40,415 - HP15_35 0.67 +1.1 40,443 + HP15_35 0.69 +0.2 40,537 + HP15_35 0.77 -0.6 40,537 + HP15_35 0.77 +0.3 40,577 + HP15_35 0.81 +0.1 40,577 - HP15_35 0.81 -0.6 40,584 + HP15_35 0.81 +0.5 40,587 - HP15_35 0.82 +0.1 40,677 - HP15_35 0.89 -0.6 40,771 - -0.5 40,815 - -0.6 40,832 + HP15_36 0.15 -0.8 40,903 + HP15_36 0.60 +0.7 40,987 - -0.7 40,987 - +0.4 40,987 - +0.2 40,995 - -1.9 41,084 + +0.3 41,115 - +0.5 41,322 + -0.1 41,535 - HP15_37 0.27 -0.2 41,551 + HP15_37 0.29 -0.6 41,626 - HP15_37 0.40 -0.5 41,645 - HP15_37 0.42 +0.8 41,712 + HP15_37 0.52 +0.2 41,752 - HP15_37 0.58 -0.9 41,779 + HP15_37 0.62 -0.0 41,779 + HP15_37 0.62 -0.2 41,811 + HP15_37 0.66 -0.0 41,928 - HP15_37 0.83 -0.8 41,938 - HP15_37 0.84 +0.0 42,021 + -0.2 42,043 + -1.5 42,051 - +0.0 42,088 + -0.3 42,131 - -0.1 42,149 + +0.1 42,156 - -2.8 42,164 + -0.0 42,164 + -0.2 42,230 + HP15_38 0.16 -0.1 42,230 + HP15_38 0.16 -0.2 42,238 - HP15_38 0.16 +0.3 42,314 - HP15_38 0.24 -1.5 42,375 + HP15_38 0.30 +0.5 42,403 + HP15_38 0.33 -0.3 42,415 + HP15_38 0.34 +0.8 42,426 - HP15_38 0.35 -1.5 42,688 + HP15_38 0.62 -0.2 42,749 - HP15_38 0.68 +0.7 42,769 + HP15_38 0.70 +0.1 42,840 + HP15_38 0.77 +1.1 42,887 + HP15_38 0.82 +0.2 42,915 + HP15_38 0.85 -0.4 42,915 + HP15_38 0.85 +0.4 42,923 - HP15_38 0.86 -0.1 42,949 + HP15_38 0.88 -0.7 42,957 - HP15_38 0.89 -0.7 43,025 - +0.6
Or see this region's nucleotide sequence