Experiment: Glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3318 and HP15_3319 are separated by 95 nucleotides HP15_3319 and HP15_3320 are separated by 41 nucleotides HP15_3320 and HP15_3321 are separated by 186 nucleotides
HP15_3318: HP15_3318 - phosphomannomutase, at 3,478,636 to 3,481,278
_3318
HP15_3319: HP15_3319 - deoxyuridine 5'-triphosphate nucleotidohydrolase Dut, at 3,481,374 to 3,481,835
_3319
HP15_3320: HP15_3320 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate-cysteine ligase, at 3,481,877 to 3,483,076
_3320
HP15_3321: HP15_3321 - DNA repair protein RadC-like protein, at 3,483,263 to 3,483,937
_3321
Position (kb)
3481
3482
3483
3484 Strain fitness (log2 ratio)
-1
0
1 at 3480.964 kb on + strand, within HP15_3318 at 3480.991 kb on + strand, within HP15_3318 at 3481.188 kb on + strand at 3481.280 kb on - strand at 3481.340 kb on - strand at 3481.340 kb on - strand at 3481.340 kb on - strand at 3481.340 kb on - strand at 3481.351 kb on + strand at 3481.841 kb on - strand at 3481.849 kb on - strand at 3483.331 kb on - strand, within HP15_3321 at 3483.406 kb on + strand, within HP15_3321 at 3483.463 kb on + strand, within HP15_3321 at 3483.479 kb on + strand, within HP15_3321 at 3483.480 kb on - strand, within HP15_3321 at 3483.674 kb on - strand, within HP15_3321 at 3483.688 kb on + strand, within HP15_3321 at 3483.787 kb on + strand, within HP15_3321 at 3483.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol remove 3,480,964 + HP15_3318 0.88 +0.0 3,480,991 + HP15_3318 0.89 +0.3 3,481,188 + -0.7 3,481,280 - +0.2 3,481,340 - +0.6 3,481,340 - -0.2 3,481,340 - -0.3 3,481,340 - +0.8 3,481,351 + +0.5 3,481,841 - -1.2 3,481,849 - +0.2 3,483,331 - HP15_3321 0.10 +0.5 3,483,406 + HP15_3321 0.21 +0.6 3,483,463 + HP15_3321 0.30 -1.0 3,483,479 + HP15_3321 0.32 +0.7 3,483,480 - HP15_3321 0.32 +1.0 3,483,674 - HP15_3321 0.61 +0.3 3,483,688 + HP15_3321 0.63 +0.6 3,483,787 + HP15_3321 0.78 +0.2 3,483,931 + -1.4
Or see this region's nucleotide sequence