Experiment: Glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_281 and HP15_282 are separated by 6 nucleotides HP15_282 and HP15_283 are separated by 2 nucleotides HP15_283 and HP15_284 overlap by 4 nucleotides
HP15_281: HP15_281 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 272,476 to 273,075
_281
HP15_282: HP15_282 - conserved hypothetical protein, secreted, at 273,082 to 273,537
_282
HP15_283: HP15_283 - methionine biosynthesis protein MetW, at 273,540 to 274,139
_283
HP15_284: HP15_284 - homoserine O-acetyltransferase, at 274,136 to 275,284
_284
Position (kb) 273
274
275 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 272.636 kb on - strand, within HP15_281 at 272.636 kb on - strand, within HP15_281 at 272.654 kb on - strand, within HP15_281 at 272.716 kb on + strand, within HP15_281 at 272.790 kb on - strand, within HP15_281 at 273.079 kb on + strand at 273.087 kb on - strand at 273.104 kb on - strand at 273.105 kb on - strand at 273.134 kb on - strand, within HP15_282 at 273.282 kb on - strand, within HP15_282 at 273.350 kb on + strand, within HP15_282 at 273.354 kb on - strand, within HP15_282 at 273.379 kb on - strand, within HP15_282 at 273.404 kb on + strand, within HP15_282 at 273.409 kb on + strand, within HP15_282 at 273.417 kb on - strand, within HP15_282 at 273.417 kb on - strand, within HP15_282 at 273.417 kb on - strand, within HP15_282 at 273.419 kb on + strand, within HP15_282 at 273.419 kb on + strand, within HP15_282 at 273.422 kb on - strand, within HP15_282 at 273.428 kb on + strand, within HP15_282 at 273.434 kb on - strand, within HP15_282 at 273.436 kb on - strand, within HP15_282 at 273.483 kb on - strand, within HP15_282 at 273.701 kb on + strand, within HP15_283 at 273.709 kb on - strand, within HP15_283 at 273.709 kb on - strand, within HP15_283 at 273.854 kb on - strand, within HP15_283 at 273.917 kb on + strand, within HP15_283 at 273.932 kb on + strand, within HP15_283 at 273.932 kb on + strand, within HP15_283 at 274.088 kb on - strand at 274.135 kb on + strand at 274.222 kb on + strand at 274.231 kb on - strand at 274.248 kb on - strand at 274.323 kb on + strand, within HP15_284 at 274.407 kb on + strand, within HP15_284 at 274.410 kb on - strand, within HP15_284 at 274.410 kb on - strand, within HP15_284 at 274.410 kb on - strand, within HP15_284 at 274.425 kb on - strand, within HP15_284 at 274.573 kb on - strand, within HP15_284 at 274.612 kb on + strand, within HP15_284 at 274.612 kb on + strand, within HP15_284 at 274.620 kb on - strand, within HP15_284 at 274.786 kb on - strand, within HP15_284 at 274.983 kb on - strand, within HP15_284 at 275.026 kb on + strand, within HP15_284 at 275.074 kb on - strand, within HP15_284
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol remove 272,636 - HP15_281 0.27 +0.2 272,636 - HP15_281 0.27 -1.4 272,654 - HP15_281 0.30 -0.1 272,716 + HP15_281 0.40 +0.2 272,790 - HP15_281 0.52 -0.5 273,079 + -0.1 273,087 - +0.3 273,104 - -0.1 273,105 - +0.0 273,134 - HP15_282 0.11 +0.2 273,282 - HP15_282 0.44 -0.1 273,350 + HP15_282 0.59 +0.1 273,354 - HP15_282 0.60 +1.5 273,379 - HP15_282 0.65 +0.4 273,404 + HP15_282 0.71 -0.1 273,409 + HP15_282 0.72 -0.4 273,417 - HP15_282 0.73 +1.8 273,417 - HP15_282 0.73 -0.4 273,417 - HP15_282 0.73 +0.4 273,419 + HP15_282 0.74 +0.1 273,419 + HP15_282 0.74 -1.1 273,422 - HP15_282 0.75 +1.0 273,428 + HP15_282 0.76 +0.0 273,434 - HP15_282 0.77 +0.8 273,436 - HP15_282 0.78 +0.2 273,483 - HP15_282 0.88 -0.1 273,701 + HP15_283 0.27 -3.7 273,709 - HP15_283 0.28 -2.5 273,709 - HP15_283 0.28 -3.2 273,854 - HP15_283 0.52 -3.0 273,917 + HP15_283 0.63 -0.5 273,932 + HP15_283 0.65 -3.1 273,932 + HP15_283 0.65 -2.3 274,088 - -2.6 274,135 + -2.5 274,222 + -4.7 274,231 - -4.6 274,248 - -4.5 274,323 + HP15_284 0.16 -2.9 274,407 + HP15_284 0.24 -4.5 274,410 - HP15_284 0.24 -3.4 274,410 - HP15_284 0.24 -4.5 274,410 - HP15_284 0.24 -5.0 274,425 - HP15_284 0.25 -3.7 274,573 - HP15_284 0.38 -2.1 274,612 + HP15_284 0.41 -2.5 274,612 + HP15_284 0.41 -6.2 274,620 - HP15_284 0.42 -3.4 274,786 - HP15_284 0.57 -4.8 274,983 - HP15_284 0.74 -3.5 275,026 + HP15_284 0.77 -4.7 275,074 - HP15_284 0.82 -3.7
Or see this region's nucleotide sequence