Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18625

Experiment: L-Tryptophan (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18620 and BWI76_RS18625 are separated by 78 nucleotidesBWI76_RS18625 and BWI76_RS18630 are separated by 106 nucleotides BWI76_RS18620: BWI76_RS18620 - L,D-transpeptidase, at 3,860,807 to 3,861,748 _RS18620 BWI76_RS18625: BWI76_RS18625 - CysB family transcriptional regulator, at 3,861,827 to 3,862,777 _RS18625 BWI76_RS18630: BWI76_RS18630 - nitrogen assimilation transcriptional regulator, at 3,862,884 to 3,863,801 _RS18630 Position (kb) 3861 3862 3863Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3860.867 kb on + strandat 3860.966 kb on + strand, within BWI76_RS18620at 3861.131 kb on + strand, within BWI76_RS18620at 3861.139 kb on - strand, within BWI76_RS18620at 3861.321 kb on + strand, within BWI76_RS18620at 3861.481 kb on + strand, within BWI76_RS18620at 3861.506 kb on + strand, within BWI76_RS18620at 3861.526 kb on + strand, within BWI76_RS18620at 3861.534 kb on - strand, within BWI76_RS18620at 3861.534 kb on - strand, within BWI76_RS18620at 3861.534 kb on - strand, within BWI76_RS18620at 3861.535 kb on - strand, within BWI76_RS18620at 3861.575 kb on + strand, within BWI76_RS18620at 3861.594 kb on + strandat 3861.675 kb on + strandat 3861.723 kb on + strandat 3861.723 kb on + strandat 3861.839 kb on + strandat 3861.980 kb on - strand, within BWI76_RS18625at 3862.062 kb on - strand, within BWI76_RS18625at 3862.090 kb on - strand, within BWI76_RS18625at 3862.142 kb on + strand, within BWI76_RS18625at 3862.148 kb on + strand, within BWI76_RS18625at 3862.150 kb on - strand, within BWI76_RS18625at 3862.150 kb on - strand, within BWI76_RS18625at 3862.399 kb on - strand, within BWI76_RS18625at 3862.400 kb on - strand, within BWI76_RS18625at 3862.434 kb on - strand, within BWI76_RS18625at 3862.457 kb on + strand, within BWI76_RS18625at 3862.472 kb on + strand, within BWI76_RS18625at 3862.485 kb on - strand, within BWI76_RS18625at 3862.584 kb on + strand, within BWI76_RS18625at 3862.685 kb on + strandat 3862.695 kb on + strandat 3862.703 kb on - strandat 3862.724 kb on - strandat 3862.776 kb on + strandat 3862.793 kb on + strandat 3862.962 kb on + strandat 3862.962 kb on + strandat 3862.970 kb on - strandat 3862.994 kb on + strand, within BWI76_RS18630at 3863.006 kb on + strand, within BWI76_RS18630at 3863.016 kb on + strand, within BWI76_RS18630at 3863.024 kb on - strand, within BWI76_RS18630at 3863.039 kb on - strand, within BWI76_RS18630at 3863.054 kb on - strand, within BWI76_RS18630at 3863.368 kb on + strand, within BWI76_RS18630at 3863.376 kb on - strand, within BWI76_RS18630at 3863.423 kb on + strand, within BWI76_RS18630at 3863.545 kb on - strand, within BWI76_RS18630at 3863.591 kb on - strand, within BWI76_RS18630at 3863.740 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Tryptophan (N)
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3,860,867 + +0.9
3,860,966 + BWI76_RS18620 0.17 -2.6
3,861,131 + BWI76_RS18620 0.34 +0.3
3,861,139 - BWI76_RS18620 0.35 +0.7
3,861,321 + BWI76_RS18620 0.55 +1.6
3,861,481 + BWI76_RS18620 0.72 +1.3
3,861,506 + BWI76_RS18620 0.74 +0.6
3,861,526 + BWI76_RS18620 0.76 -0.3
3,861,534 - BWI76_RS18620 0.77 -2.0
3,861,534 - BWI76_RS18620 0.77 +0.4
3,861,534 - BWI76_RS18620 0.77 -0.4
3,861,535 - BWI76_RS18620 0.77 +0.1
3,861,575 + BWI76_RS18620 0.82 -0.7
3,861,594 + -0.6
3,861,675 + -0.2
3,861,723 + +2.0
3,861,723 + +0.6
3,861,839 + -0.8
3,861,980 - BWI76_RS18625 0.16 +0.1
3,862,062 - BWI76_RS18625 0.25 +0.2
3,862,090 - BWI76_RS18625 0.28 -0.1
3,862,142 + BWI76_RS18625 0.33 -0.1
3,862,148 + BWI76_RS18625 0.34 -0.3
3,862,150 - BWI76_RS18625 0.34 -0.1
3,862,150 - BWI76_RS18625 0.34 +0.8
3,862,399 - BWI76_RS18625 0.60 -0.0
3,862,400 - BWI76_RS18625 0.60 -0.1
3,862,434 - BWI76_RS18625 0.64 +2.0
3,862,457 + BWI76_RS18625 0.66 +1.2
3,862,472 + BWI76_RS18625 0.68 -2.1
3,862,485 - BWI76_RS18625 0.69 +0.3
3,862,584 + BWI76_RS18625 0.80 -1.0
3,862,685 + -0.2
3,862,695 + +0.2
3,862,703 - -0.2
3,862,724 - +0.2
3,862,776 + -1.7
3,862,793 + -1.3
3,862,962 + -0.7
3,862,962 + -1.6
3,862,970 - -3.2
3,862,994 + BWI76_RS18630 0.12 -1.9
3,863,006 + BWI76_RS18630 0.13 -3.1
3,863,016 + BWI76_RS18630 0.14 -2.0
3,863,024 - BWI76_RS18630 0.15 -1.2
3,863,039 - BWI76_RS18630 0.17 -3.4
3,863,054 - BWI76_RS18630 0.19 -1.7
3,863,368 + BWI76_RS18630 0.53 -2.9
3,863,376 - BWI76_RS18630 0.54 +1.0
3,863,423 + BWI76_RS18630 0.59 -1.0
3,863,545 - BWI76_RS18630 0.72 -2.7
3,863,591 - BWI76_RS18630 0.77 -2.1
3,863,740 + -2.7

Or see this region's nucleotide sequence