Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13990 and BWI76_RS13995 are separated by 184 nucleotides BWI76_RS13995 and BWI76_RS14000 are separated by 103 nucleotides BWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotides
BWI76_RS13990: BWI76_RS13990 - allophanate hydrolase, at 2,921,893 to 2,923,689
_RS13990
BWI76_RS13995: BWI76_RS13995 - penicillin-binding protein 2, at 2,923,874 to 2,925,793
_RS13995
BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640
_RS14000
BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191
_RS14005
Position (kb)
2923
2924
2925
2926 Strain fitness (log2 ratio)
-1
0
1 at 2922.966 kb on + strand, within BWI76_RS13990 at 2923.183 kb on - strand, within BWI76_RS13990 at 2923.518 kb on - strand at 2924.282 kb on - strand, within BWI76_RS13995 at 2924.454 kb on - strand, within BWI76_RS13995 at 2924.576 kb on + strand, within BWI76_RS13995 at 2924.944 kb on + strand, within BWI76_RS13995 at 2925.323 kb on - strand, within BWI76_RS13995 at 2925.419 kb on - strand, within BWI76_RS13995 at 2925.519 kb on - strand, within BWI76_RS13995 at 2926.067 kb on + strand, within BWI76_RS14000 at 2926.339 kb on - strand, within BWI76_RS14000 at 2926.343 kb on + strand, within BWI76_RS14000 at 2926.523 kb on - strand, within BWI76_RS14000 at 2926.570 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 2,922,966 + BWI76_RS13990 0.60 +0.1 2,923,183 - BWI76_RS13990 0.72 -0.5 2,923,518 - +1.0 2,924,282 - BWI76_RS13995 0.21 -0.6 2,924,454 - BWI76_RS13995 0.30 +0.1 2,924,576 + BWI76_RS13995 0.37 +0.2 2,924,944 + BWI76_RS13995 0.56 -0.2 2,925,323 - BWI76_RS13995 0.75 +0.4 2,925,419 - BWI76_RS13995 0.80 +0.9 2,925,519 - BWI76_RS13995 0.86 +0.2 2,926,067 + BWI76_RS14000 0.23 -0.2 2,926,339 - BWI76_RS14000 0.59 +0.8 2,926,343 + BWI76_RS14000 0.60 -0.4 2,926,523 - BWI76_RS14000 0.84 +0.3 2,926,570 + +0.0
Or see this region's nucleotide sequence