Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06110 and BWI76_RS06115 overlap by 4 nucleotides BWI76_RS06115 and BWI76_RS06120 are separated by 255 nucleotides BWI76_RS06120 and BWI76_RS06125 are separated by 151 nucleotides BWI76_RS06125 and BWI76_RS06130 are separated by 101 nucleotides BWI76_RS06130 and BWI76_RS06135 are separated by 169 nucleotides
BWI76_RS06110: BWI76_RS06110 - hypothetical protein, at 1,284,849 to 1,285,955
_RS06110
BWI76_RS06115: BWI76_RS06115 - membrane protein, at 1,285,952 to 1,286,254
_RS06115
BWI76_RS06120: BWI76_RS06120 - membrane protein, at 1,286,510 to 1,286,713
_RS06120
BWI76_RS06125: BWI76_RS06125 - D-alanine--D-alanine ligase A, at 1,286,865 to 1,287,962
_RS06125
BWI76_RS06130: BWI76_RS06130 - extensin family protein, at 1,288,064 to 1,288,747
_RS06130
BWI76_RS06135: BWI76_RS06135 - MFS transporter, at 1,288,917 to 1,290,116
_RS06135
Position (kb)
1286
1287
1288 Strain fitness (log2 ratio)
-1
0
1
2 at 1285.944 kb on - strand at 1286.548 kb on - strand, within BWI76_RS06120 at 1286.553 kb on - strand, within BWI76_RS06120 at 1286.766 kb on + strand at 1286.965 kb on + strand at 1286.972 kb on + strand at 1287.054 kb on - strand, within BWI76_RS06125 at 1287.253 kb on + strand, within BWI76_RS06125 at 1287.364 kb on + strand, within BWI76_RS06125 at 1287.477 kb on + strand, within BWI76_RS06125 at 1287.490 kb on - strand, within BWI76_RS06125 at 1287.506 kb on + strand, within BWI76_RS06125 at 1287.657 kb on + strand, within BWI76_RS06125 at 1287.705 kb on + strand, within BWI76_RS06125 at 1287.760 kb on - strand, within BWI76_RS06125 at 1287.766 kb on - strand, within BWI76_RS06125 at 1287.990 kb on + strand at 1288.076 kb on + strand at 1288.122 kb on + strand at 1288.615 kb on + strand, within BWI76_RS06130 at 1288.623 kb on - strand, within BWI76_RS06130 at 1288.768 kb on - strand at 1288.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 1,285,944 - +0.2 1,286,548 - BWI76_RS06120 0.19 +0.1 1,286,553 - BWI76_RS06120 0.21 +0.4 1,286,766 + -0.2 1,286,965 + +1.0 1,286,972 + +0.2 1,287,054 - BWI76_RS06125 0.17 +0.9 1,287,253 + BWI76_RS06125 0.35 +2.2 1,287,364 + BWI76_RS06125 0.45 +0.6 1,287,477 + BWI76_RS06125 0.56 +0.6 1,287,490 - BWI76_RS06125 0.57 +1.3 1,287,506 + BWI76_RS06125 0.58 +1.0 1,287,657 + BWI76_RS06125 0.72 +1.2 1,287,705 + BWI76_RS06125 0.77 +1.3 1,287,760 - BWI76_RS06125 0.82 +1.0 1,287,766 - BWI76_RS06125 0.82 -0.0 1,287,990 + +1.4 1,288,076 + +0.2 1,288,122 + +0.9 1,288,615 + BWI76_RS06130 0.81 -0.4 1,288,623 - BWI76_RS06130 0.82 -0.2 1,288,768 - +1.6 1,288,768 - +0.3
Or see this region's nucleotide sequence