Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05975

Experiment: L-Tryptophan (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05960 and BWI76_RS05965 overlap by 75 nucleotidesBWI76_RS05965 and BWI76_RS05970 are separated by 70 nucleotidesBWI76_RS05970 and BWI76_RS05975 are separated by 107 nucleotidesBWI76_RS05975 and BWI76_RS05980 overlap by 8 nucleotidesBWI76_RS05980 and BWI76_RS05985 overlap by 14 nucleotides BWI76_RS05960: BWI76_RS05960 - cysteine transport system periplasmic binding protein, at 1,252,622 to 1,253,431 _RS05960 BWI76_RS05965: BWI76_RS05965 - hypothetical protein, at 1,253,357 to 1,253,674 _RS05965 BWI76_RS05970: BWI76_RS05970 - hypothetical protein, at 1,253,745 to 1,253,927 _RS05970 BWI76_RS05975: BWI76_RS05975 - ABC transporter ATP-binding protein, at 1,254,035 to 1,254,730 _RS05975 BWI76_RS05980: BWI76_RS05980 - ABC transporter ATP-binding protein, at 1,254,723 to 1,255,505 _RS05980 BWI76_RS05985: BWI76_RS05985 - branched-chain amino acid ABC transporter permease, at 1,255,492 to 1,256,541 _RS05985 Position (kb) 1254 1255Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1253.154 kb on - strand, within BWI76_RS05960at 1253.193 kb on + strand, within BWI76_RS05960at 1253.211 kb on - strand, within BWI76_RS05960at 1253.254 kb on - strand, within BWI76_RS05960at 1253.284 kb on + strand, within BWI76_RS05960at 1253.364 kb on + strandat 1253.364 kb on + strandat 1253.372 kb on - strandat 1253.372 kb on - strandat 1253.372 kb on - strandat 1253.372 kb on - strandat 1253.604 kb on - strand, within BWI76_RS05965at 1253.606 kb on - strand, within BWI76_RS05965at 1253.699 kb on + strandat 1253.707 kb on - strandat 1253.798 kb on + strand, within BWI76_RS05970at 1253.880 kb on - strand, within BWI76_RS05970at 1254.022 kb on + strandat 1254.030 kb on - strandat 1254.030 kb on - strandat 1254.030 kb on - strandat 1254.062 kb on - strandat 1254.255 kb on - strand, within BWI76_RS05975at 1254.403 kb on + strand, within BWI76_RS05975at 1254.411 kb on - strand, within BWI76_RS05975at 1254.432 kb on + strand, within BWI76_RS05975at 1254.675 kb on + strandat 1254.794 kb on - strandat 1254.816 kb on - strand, within BWI76_RS05980at 1254.856 kb on + strand, within BWI76_RS05980at 1255.055 kb on - strand, within BWI76_RS05980at 1255.099 kb on + strand, within BWI76_RS05980at 1255.099 kb on + strand, within BWI76_RS05980at 1255.182 kb on + strand, within BWI76_RS05980at 1255.338 kb on - strand, within BWI76_RS05980at 1255.352 kb on + strandat 1255.384 kb on + strand, within BWI76_RS05980at 1255.465 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Tryptophan (N)
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1,253,154 - BWI76_RS05960 0.66 +0.1
1,253,193 + BWI76_RS05960 0.70 +0.0
1,253,211 - BWI76_RS05960 0.73 +0.2
1,253,254 - BWI76_RS05960 0.78 +1.1
1,253,284 + BWI76_RS05960 0.82 -1.1
1,253,364 + +0.4
1,253,364 + -0.8
1,253,372 - -0.3
1,253,372 - -0.2
1,253,372 - -0.1
1,253,372 - +0.1
1,253,604 - BWI76_RS05965 0.78 -0.5
1,253,606 - BWI76_RS05965 0.78 -0.7
1,253,699 + -0.4
1,253,707 - -0.7
1,253,798 + BWI76_RS05970 0.29 -0.2
1,253,880 - BWI76_RS05970 0.74 -1.1
1,254,022 + +0.5
1,254,030 - -0.3
1,254,030 - -0.8
1,254,030 - -0.5
1,254,062 - +0.1
1,254,255 - BWI76_RS05975 0.32 -0.7
1,254,403 + BWI76_RS05975 0.53 -3.7
1,254,411 - BWI76_RS05975 0.54 -1.7
1,254,432 + BWI76_RS05975 0.57 -2.8
1,254,675 + -2.6
1,254,794 - -4.4
1,254,816 - BWI76_RS05980 0.12 -4.2
1,254,856 + BWI76_RS05980 0.17 +0.5
1,255,055 - BWI76_RS05980 0.42 -3.2
1,255,099 + BWI76_RS05980 0.48 -3.0
1,255,099 + BWI76_RS05980 0.48 -4.8
1,255,182 + BWI76_RS05980 0.59 -3.1
1,255,338 - BWI76_RS05980 0.79 -1.6
1,255,352 + +2.1
1,255,384 + BWI76_RS05980 0.84 -1.9
1,255,465 + -3.1

Or see this region's nucleotide sequence