Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05200

Experiment: L-Tryptophan (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05190 and BWI76_RS05195 are separated by 107 nucleotidesBWI76_RS05195 and BWI76_RS05200 are separated by 34 nucleotidesBWI76_RS05200 and BWI76_RS05205 are separated by 131 nucleotides BWI76_RS05190: BWI76_RS05190 - hypothetical protein, at 1,092,432 to 1,092,821 _RS05190 BWI76_RS05195: BWI76_RS05195 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,092,929 to 1,093,753 _RS05195 BWI76_RS05200: BWI76_RS05200 - bifunctional uridylyltransferase/uridylyl-removing protein, at 1,093,788 to 1,096,451 _RS05200 BWI76_RS05205: BWI76_RS05205 - type I methionyl aminopeptidase, at 1,096,583 to 1,097,377 _RS05205 Position (kb) 1093 1094 1095 1096 1097Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1092.789 kb on + strandat 1092.794 kb on + strandat 1092.802 kb on - strandat 1092.818 kb on + strandat 1092.837 kb on - strandat 1092.927 kb on + strandat 1093.792 kb on + strandat 1094.586 kb on - strand, within BWI76_RS05200at 1094.801 kb on + strand, within BWI76_RS05200at 1094.801 kb on + strand, within BWI76_RS05200at 1094.834 kb on + strand, within BWI76_RS05200at 1094.842 kb on - strand, within BWI76_RS05200at 1095.761 kb on + strand, within BWI76_RS05200at 1095.949 kb on - strand, within BWI76_RS05200at 1096.423 kb on - strandat 1096.437 kb on - strandat 1096.654 kb on - strandat 1096.672 kb on + strand, within BWI76_RS05205at 1096.689 kb on - strand, within BWI76_RS05205at 1096.786 kb on - strand, within BWI76_RS05205at 1096.826 kb on - strand, within BWI76_RS05205at 1096.826 kb on - strand, within BWI76_RS05205at 1096.840 kb on - strand, within BWI76_RS05205at 1096.988 kb on - strand, within BWI76_RS05205at 1097.052 kb on - strand, within BWI76_RS05205

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Tryptophan (N)
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1,092,789 + +0.1
1,092,794 + +0.0
1,092,802 - +0.6
1,092,818 + +1.8
1,092,837 - +0.1
1,092,927 + +0.7
1,093,792 + -1.1
1,094,586 - BWI76_RS05200 0.30 -1.2
1,094,801 + BWI76_RS05200 0.38 -1.5
1,094,801 + BWI76_RS05200 0.38 -2.5
1,094,834 + BWI76_RS05200 0.39 -0.2
1,094,842 - BWI76_RS05200 0.40 -2.6
1,095,761 + BWI76_RS05200 0.74 -1.4
1,095,949 - BWI76_RS05200 0.81 -1.1
1,096,423 - +1.1
1,096,437 - -0.7
1,096,654 - +1.2
1,096,672 + BWI76_RS05205 0.11 -3.0
1,096,689 - BWI76_RS05205 0.13 +1.3
1,096,786 - BWI76_RS05205 0.26 -1.7
1,096,826 - BWI76_RS05205 0.31 +0.6
1,096,826 - BWI76_RS05205 0.31 +1.1
1,096,840 - BWI76_RS05205 0.32 -0.4
1,096,988 - BWI76_RS05205 0.51 +0.0
1,097,052 - BWI76_RS05205 0.59 +0.1

Or see this region's nucleotide sequence