Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04845 and BWI76_RS04850 are separated by 194 nucleotides BWI76_RS04850 and BWI76_RS04855 are separated by 9 nucleotides BWI76_RS04855 and BWI76_RS04860 are separated by 31 nucleotides BWI76_RS04860 and BWI76_RS04865 overlap by 1 nucleotides
BWI76_RS04845: BWI76_RS04845 - nicotinate-nucleotide pyrophosphorylase, at 1,012,016 to 1,012,909
_RS04845
BWI76_RS04850: BWI76_RS04850 - N-acetylmuramoyl-L-alanine amidase, at 1,013,104 to 1,013,655
_RS04850
BWI76_RS04855: BWI76_RS04855 - regulatory protein AmpE, at 1,013,665 to 1,014,519
_RS04855
BWI76_RS04860: BWI76_RS04860 - alpha-N-arabinofuranosidase, at 1,014,551 to 1,015,501
_RS04860
BWI76_RS04865: BWI76_RS04865 - MFS transporter, at 1,015,501 to 1,016,907
_RS04865
Position (kb) 1013
1014
1015 Strain fitness (log2 ratio) -1
0
1
2 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.810 kb on - strand, within BWI76_RS04845 at 1013.094 kb on - strand at 1013.256 kb on + strand, within BWI76_RS04850 at 1013.368 kb on + strand, within BWI76_RS04850 at 1013.398 kb on + strand, within BWI76_RS04850 at 1013.472 kb on + strand, within BWI76_RS04850 at 1013.514 kb on - strand at 1013.613 kb on - strand at 1013.675 kb on + strand at 1013.681 kb on - strand at 1013.708 kb on + strand at 1013.716 kb on - strand at 1013.716 kb on - strand at 1013.760 kb on - strand, within BWI76_RS04855 at 1013.784 kb on + strand, within BWI76_RS04855 at 1013.844 kb on + strand, within BWI76_RS04855 at 1013.904 kb on + strand, within BWI76_RS04855 at 1013.904 kb on - strand, within BWI76_RS04855 at 1013.917 kb on + strand at 1014.080 kb on - strand, within BWI76_RS04855 at 1014.278 kb on + strand, within BWI76_RS04855 at 1014.395 kb on - strand, within BWI76_RS04855 at 1014.681 kb on + strand, within BWI76_RS04860 at 1014.735 kb on + strand, within BWI76_RS04860 at 1014.748 kb on - strand, within BWI76_RS04860 at 1014.824 kb on - strand, within BWI76_RS04860 at 1014.841 kb on - strand, within BWI76_RS04860 at 1015.293 kb on - strand, within BWI76_RS04860 at 1015.293 kb on - strand, within BWI76_RS04860 at 1015.366 kb on + strand, within BWI76_RS04860 at 1015.374 kb on - strand, within BWI76_RS04860 at 1015.400 kb on + strand, within BWI76_RS04860 at 1015.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 1,012,802 + BWI76_RS04845 0.88 +0.5 1,012,802 + BWI76_RS04845 0.88 -0.1 1,012,810 - BWI76_RS04845 0.89 -0.1 1,013,094 - -0.6 1,013,256 + BWI76_RS04850 0.28 +0.1 1,013,368 + BWI76_RS04850 0.48 -0.3 1,013,398 + BWI76_RS04850 0.53 -0.6 1,013,472 + BWI76_RS04850 0.67 -0.1 1,013,514 - +0.1 1,013,613 - -0.1 1,013,675 + +0.1 1,013,681 - +0.3 1,013,708 + +0.0 1,013,716 - +0.4 1,013,716 - -0.3 1,013,760 - BWI76_RS04855 0.11 +0.5 1,013,784 + BWI76_RS04855 0.14 +0.6 1,013,844 + BWI76_RS04855 0.21 +0.4 1,013,904 + BWI76_RS04855 0.28 +0.4 1,013,904 - BWI76_RS04855 0.28 +0.2 1,013,917 + +1.1 1,014,080 - BWI76_RS04855 0.49 +0.1 1,014,278 + BWI76_RS04855 0.72 +1.4 1,014,395 - BWI76_RS04855 0.85 +1.1 1,014,681 + BWI76_RS04860 0.14 -0.9 1,014,735 + BWI76_RS04860 0.19 -0.5 1,014,748 - BWI76_RS04860 0.21 -0.0 1,014,824 - BWI76_RS04860 0.29 -0.1 1,014,841 - BWI76_RS04860 0.30 -0.2 1,015,293 - BWI76_RS04860 0.78 -0.1 1,015,293 - BWI76_RS04860 0.78 -0.5 1,015,366 + BWI76_RS04860 0.86 -0.5 1,015,374 - BWI76_RS04860 0.87 -0.3 1,015,400 + BWI76_RS04860 0.89 +2.1 1,015,493 + -0.2
Or see this region's nucleotide sequence