Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04455 and BWI76_RS04460 are separated by 247 nucleotides BWI76_RS04460 and BWI76_RS04465 are separated by 457 nucleotides
BWI76_RS04455: BWI76_RS04455 - triphosphoribosyl-dephospho-CoA synthase CitG, at 920,111 to 921,019
_RS04455
BWI76_RS04460: BWI76_RS04460 - 4-hydroxy-tetrahydrodipicolinate reductase, at 921,267 to 922,088
_RS04460
BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694
_RS04465
Position (kb) 921
922
923 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 920.281 kb on - strand, within BWI76_RS04455 at 920.283 kb on + strand, within BWI76_RS04455 at 920.350 kb on + strand, within BWI76_RS04455 at 920.372 kb on - strand, within BWI76_RS04455 at 920.479 kb on - strand, within BWI76_RS04455 at 920.497 kb on - strand, within BWI76_RS04455 at 920.689 kb on + strand, within BWI76_RS04455 at 920.728 kb on + strand, within BWI76_RS04455 at 920.873 kb on + strand, within BWI76_RS04455 at 920.952 kb on + strand at 921.142 kb on - strand at 922.434 kb on + strand at 922.440 kb on - strand at 922.440 kb on - strand at 922.599 kb on + strand at 922.828 kb on + strand, within BWI76_RS04465 at 922.836 kb on + strand, within BWI76_RS04465 at 922.836 kb on - strand, within BWI76_RS04465 at 922.897 kb on - strand, within BWI76_RS04465 at 923.052 kb on - strand, within BWI76_RS04465
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 920,281 - BWI76_RS04455 0.19 -1.3 920,283 + BWI76_RS04455 0.19 -0.5 920,350 + BWI76_RS04455 0.26 +0.4 920,372 - BWI76_RS04455 0.29 +0.8 920,479 - BWI76_RS04455 0.40 -0.1 920,497 - BWI76_RS04455 0.42 -0.4 920,689 + BWI76_RS04455 0.64 -3.2 920,728 + BWI76_RS04455 0.68 -0.3 920,873 + BWI76_RS04455 0.84 -0.8 920,952 + +0.2 921,142 - -0.0 922,434 + -0.4 922,440 - +1.2 922,440 - +0.9 922,599 + -3.2 922,828 + BWI76_RS04465 0.25 -4.9 922,836 + BWI76_RS04465 0.25 -4.7 922,836 - BWI76_RS04465 0.25 -3.9 922,897 - BWI76_RS04465 0.31 -1.2 923,052 - BWI76_RS04465 0.44 -3.9
Or see this region's nucleotide sequence