Experiment: reconstituted MRS with WakoIMO-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14575 and BBR_RS20835 are separated by 174 nucleotides BBR_RS20835 and BBR_RS14585 are separated by 7 nucleotides BBR_RS14585 and BBR_RS14590 are separated by 99 nucleotides BBR_RS14590 and BBR_RS14595 are separated by 613 nucleotides
BBR_RS14575: BBR_RS14575 - YraN family protein, at 1,043,833 to 1,044,249
_RS14575
BBR_RS20835: BBR_RS20835 - hypothetical protein, at 1,044,424 to 1,044,884
_RS20835
BBR_RS14585: BBR_RS14585 - hypothetical protein, at 1,044,892 to 1,045,122
_RS14585
BBR_RS14590: BBR_RS14590 - pyridoxine kinase, at 1,045,222 to 1,046,094
_RS14590
BBR_RS14595: BBR_RS14595 - O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase, at 1,046,708 to 1,048,024
_RS14595
Position (kb)
1045
1046
1047 Strain fitness (log2 ratio)
-1
0
1 at 1044.257 kb on + strand at 1044.308 kb on - strand at 1044.327 kb on + strand at 1044.327 kb on + strand at 1044.327 kb on + strand at 1044.335 kb on - strand at 1044.370 kb on - strand at 1044.464 kb on + strand at 1044.471 kb on + strand, within BBR_RS20835 at 1044.501 kb on - strand, within BBR_RS20835 at 1044.510 kb on + strand, within BBR_RS20835 at 1044.529 kb on + strand, within BBR_RS20835 at 1044.529 kb on + strand, within BBR_RS20835 at 1044.535 kb on - strand, within BBR_RS20835 at 1044.631 kb on + strand, within BBR_RS20835 at 1044.631 kb on + strand, within BBR_RS20835 at 1044.758 kb on + strand, within BBR_RS20835 at 1044.815 kb on + strand, within BBR_RS20835 at 1044.815 kb on + strand, within BBR_RS20835 at 1044.874 kb on + strand at 1044.874 kb on + strand at 1044.874 kb on + strand at 1044.874 kb on + strand at 1044.874 kb on - strand at 1044.888 kb on - strand at 1044.905 kb on + strand at 1044.905 kb on + strand at 1045.003 kb on - strand, within BBR_RS14585 at 1045.025 kb on - strand, within BBR_RS14585 at 1045.061 kb on - strand, within BBR_RS14585 at 1045.175 kb on - strand at 1045.210 kb on + strand at 1045.289 kb on + strand at 1045.354 kb on - strand, within BBR_RS14590 at 1045.364 kb on - strand, within BBR_RS14590 at 1045.386 kb on + strand, within BBR_RS14590 at 1045.394 kb on - strand, within BBR_RS14590 at 1045.498 kb on - strand, within BBR_RS14590 at 1045.562 kb on + strand, within BBR_RS14590 at 1045.562 kb on + strand, within BBR_RS14590 at 1045.685 kb on + strand, within BBR_RS14590 at 1045.693 kb on - strand, within BBR_RS14590 at 1045.693 kb on - strand, within BBR_RS14590 at 1045.695 kb on + strand, within BBR_RS14590 at 1045.695 kb on + strand, within BBR_RS14590 at 1045.695 kb on + strand, within BBR_RS14590 at 1045.703 kb on - strand, within BBR_RS14590 at 1045.703 kb on - strand, within BBR_RS14590 at 1045.712 kb on + strand, within BBR_RS14590 at 1045.740 kb on - strand, within BBR_RS14590 at 1045.740 kb on - strand, within BBR_RS14590 at 1045.745 kb on - strand, within BBR_RS14590 at 1045.756 kb on - strand, within BBR_RS14590 at 1045.803 kb on + strand, within BBR_RS14590 at 1045.811 kb on - strand, within BBR_RS14590 at 1045.813 kb on + strand, within BBR_RS14590 at 1045.837 kb on + strand, within BBR_RS14590 at 1045.850 kb on - strand, within BBR_RS14590 at 1045.906 kb on + strand, within BBR_RS14590 at 1045.919 kb on + strand, within BBR_RS14590 at 1045.931 kb on + strand, within BBR_RS14590 at 1045.931 kb on + strand, within BBR_RS14590 at 1045.944 kb on - strand, within BBR_RS14590 at 1046.056 kb on - strand at 1046.079 kb on - strand at 1046.145 kb on + strand at 1046.153 kb on - strand at 1046.165 kb on + strand at 1046.286 kb on - strand at 1046.343 kb on - strand at 1046.384 kb on - strand at 1046.410 kb on - strand at 1046.466 kb on + strand at 1046.474 kb on - strand at 1046.559 kb on + strand at 1046.560 kb on + strand at 1046.569 kb on - strand at 1046.616 kb on + strand at 1046.640 kb on + strand at 1046.710 kb on + strand at 1046.744 kb on + strand at 1046.750 kb on - strand at 1046.798 kb on + strand at 1046.798 kb on + strand at 1046.806 kb on - strand at 1046.835 kb on + strand at 1046.843 kb on - strand, within BBR_RS14595 at 1046.882 kb on + strand, within BBR_RS14595 at 1046.889 kb on - strand, within BBR_RS14595 at 1046.889 kb on - strand, within BBR_RS14595 at 1046.889 kb on - strand, within BBR_RS14595 at 1046.896 kb on + strand, within BBR_RS14595 at 1046.899 kb on - strand, within BBR_RS14595 at 1046.901 kb on + strand, within BBR_RS14595 at 1046.901 kb on + strand, within BBR_RS14595 at 1046.909 kb on - strand, within BBR_RS14595 at 1046.909 kb on - strand, within BBR_RS14595 at 1046.946 kb on - strand, within BBR_RS14595 at 1047.006 kb on + strand, within BBR_RS14595 at 1047.064 kb on - strand, within BBR_RS14595 at 1047.068 kb on + strand, within BBR_RS14595 at 1047.073 kb on + strand, within BBR_RS14595 at 1047.083 kb on - strand, within BBR_RS14595 at 1047.083 kb on - strand, within BBR_RS14595
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with WakoIMO-prebiotic 0.5 %w/v remove 1,044,257 + +0.4 1,044,308 - +0.8 1,044,327 + +1.2 1,044,327 + +0.5 1,044,327 + -0.3 1,044,335 - +0.6 1,044,370 - +0.2 1,044,464 + +0.4 1,044,471 + BBR_RS20835 0.10 +0.5 1,044,501 - BBR_RS20835 0.17 +0.2 1,044,510 + BBR_RS20835 0.19 +0.2 1,044,529 + BBR_RS20835 0.23 +0.1 1,044,529 + BBR_RS20835 0.23 -0.3 1,044,535 - BBR_RS20835 0.24 +0.4 1,044,631 + BBR_RS20835 0.45 +0.1 1,044,631 + BBR_RS20835 0.45 +1.1 1,044,758 + BBR_RS20835 0.72 -0.5 1,044,815 + BBR_RS20835 0.85 +0.3 1,044,815 + BBR_RS20835 0.85 -0.0 1,044,874 + +0.4 1,044,874 + +0.6 1,044,874 + -0.3 1,044,874 + +0.0 1,044,874 - -0.1 1,044,888 - +0.1 1,044,905 + +0.6 1,044,905 + +0.3 1,045,003 - BBR_RS14585 0.48 +0.4 1,045,025 - BBR_RS14585 0.58 -0.7 1,045,061 - BBR_RS14585 0.73 +1.2 1,045,175 - +1.5 1,045,210 + +0.3 1,045,289 + +0.0 1,045,354 - BBR_RS14590 0.15 +0.3 1,045,364 - BBR_RS14590 0.16 -0.0 1,045,386 + BBR_RS14590 0.19 +0.7 1,045,394 - BBR_RS14590 0.20 -0.1 1,045,498 - BBR_RS14590 0.32 +0.3 1,045,562 + BBR_RS14590 0.39 +0.6 1,045,562 + BBR_RS14590 0.39 +0.0 1,045,685 + BBR_RS14590 0.53 -0.1 1,045,693 - BBR_RS14590 0.54 -0.1 1,045,693 - BBR_RS14590 0.54 -0.8 1,045,695 + BBR_RS14590 0.54 -0.5 1,045,695 + BBR_RS14590 0.54 +0.4 1,045,695 + BBR_RS14590 0.54 -0.2 1,045,703 - BBR_RS14590 0.55 -0.5 1,045,703 - BBR_RS14590 0.55 +0.3 1,045,712 + BBR_RS14590 0.56 -0.3 1,045,740 - BBR_RS14590 0.59 +0.2 1,045,740 - BBR_RS14590 0.59 +0.2 1,045,745 - BBR_RS14590 0.60 -0.8 1,045,756 - BBR_RS14590 0.61 -0.1 1,045,803 + BBR_RS14590 0.67 +0.5 1,045,811 - BBR_RS14590 0.67 +0.7 1,045,813 + BBR_RS14590 0.68 +1.0 1,045,837 + BBR_RS14590 0.70 +0.7 1,045,850 - BBR_RS14590 0.72 -0.7 1,045,906 + BBR_RS14590 0.78 +1.0 1,045,919 + BBR_RS14590 0.80 +1.0 1,045,931 + BBR_RS14590 0.81 +0.6 1,045,931 + BBR_RS14590 0.81 +0.2 1,045,944 - BBR_RS14590 0.83 +0.4 1,046,056 - +0.0 1,046,079 - -0.0 1,046,145 + -1.5 1,046,153 - -0.3 1,046,165 + +0.2 1,046,286 - +0.3 1,046,343 - +0.0 1,046,384 - +0.3 1,046,410 - -0.4 1,046,466 + +0.0 1,046,474 - -0.5 1,046,559 + -0.5 1,046,560 + +0.1 1,046,569 - -0.6 1,046,616 + -0.0 1,046,640 + +0.1 1,046,710 + +0.0 1,046,744 + -0.1 1,046,750 - +0.8 1,046,798 + -0.2 1,046,798 + -0.1 1,046,806 - +0.3 1,046,835 + +0.2 1,046,843 - BBR_RS14595 0.10 +0.1 1,046,882 + BBR_RS14595 0.13 -0.1 1,046,889 - BBR_RS14595 0.14 +0.6 1,046,889 - BBR_RS14595 0.14 +0.2 1,046,889 - BBR_RS14595 0.14 +0.0 1,046,896 + BBR_RS14595 0.14 -0.1 1,046,899 - BBR_RS14595 0.15 -0.4 1,046,901 + BBR_RS14595 0.15 +0.2 1,046,901 + BBR_RS14595 0.15 +0.5 1,046,909 - BBR_RS14595 0.15 +0.7 1,046,909 - BBR_RS14595 0.15 -0.2 1,046,946 - BBR_RS14595 0.18 +0.1 1,047,006 + BBR_RS14595 0.23 -0.5 1,047,064 - BBR_RS14595 0.27 +0.3 1,047,068 + BBR_RS14595 0.27 +0.8 1,047,073 + BBR_RS14595 0.28 +0.6 1,047,083 - BBR_RS14595 0.28 -1.0 1,047,083 - BBR_RS14595 0.28 -1.2
Or see this region's nucleotide sequence