Experiment: reconstituted MRS with WakoIMO-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11175 and BBR_RS11180 are separated by 383 nucleotides BBR_RS11180 and BBR_RS11185 are separated by 103 nucleotides
BBR_RS11175: BBR_RS11175 - penicillin-binding protein, at 248,656 to 251,010
_RS11175
BBR_RS11180: BBR_RS11180 - phosphatase PAP2 family protein, at 251,394 to 252,572
_RS11180
BBR_RS11185: BBR_RS11185 - type I DNA topoisomerase, at 252,676 to 255,726
_RS11185
Position (kb) 251
252
253 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 250.402 kb on + strand, within BBR_RS11175 at 250.410 kb on - strand, within BBR_RS11175 at 250.413 kb on + strand, within BBR_RS11175 at 250.441 kb on - strand, within BBR_RS11175 at 250.441 kb on - strand, within BBR_RS11175 at 250.441 kb on - strand, within BBR_RS11175 at 250.441 kb on - strand, within BBR_RS11175 at 250.453 kb on + strand, within BBR_RS11175 at 250.453 kb on + strand, within BBR_RS11175 at 250.455 kb on + strand, within BBR_RS11175 at 250.461 kb on - strand, within BBR_RS11175 at 250.465 kb on + strand, within BBR_RS11175 at 250.501 kb on + strand, within BBR_RS11175 at 250.501 kb on + strand, within BBR_RS11175 at 250.501 kb on + strand, within BBR_RS11175 at 250.516 kb on + strand, within BBR_RS11175 at 250.524 kb on - strand, within BBR_RS11175 at 250.524 kb on - strand, within BBR_RS11175 at 250.547 kb on + strand, within BBR_RS11175 at 250.547 kb on + strand, within BBR_RS11175 at 250.547 kb on + strand, within BBR_RS11175 at 250.555 kb on - strand, within BBR_RS11175 at 250.555 kb on - strand, within BBR_RS11175 at 250.647 kb on - strand, within BBR_RS11175 at 250.667 kb on + strand, within BBR_RS11175 at 250.675 kb on - strand, within BBR_RS11175 at 250.727 kb on + strand, within BBR_RS11175 at 250.727 kb on + strand, within BBR_RS11175 at 250.730 kb on + strand, within BBR_RS11175 at 250.738 kb on - strand, within BBR_RS11175 at 250.772 kb on + strand, within BBR_RS11175 at 250.790 kb on - strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.808 kb on - strand at 250.856 kb on + strand at 250.856 kb on + strand at 250.856 kb on + strand at 250.864 kb on - strand at 250.864 kb on - strand at 250.870 kb on - strand at 250.877 kb on + strand at 250.931 kb on + strand at 250.931 kb on + strand at 250.939 kb on - strand at 250.939 kb on - strand at 250.939 kb on - strand at 250.939 kb on - strand at 250.939 kb on - strand at 250.949 kb on - strand at 250.960 kb on - strand at 250.960 kb on - strand at 250.960 kb on - strand at 250.967 kb on + strand at 250.969 kb on + strand at 250.979 kb on + strand at 250.986 kb on + strand at 251.016 kb on + strand at 251.042 kb on + strand at 251.050 kb on - strand at 251.057 kb on + strand at 251.057 kb on + strand at 251.057 kb on - strand at 251.057 kb on - strand at 251.064 kb on + strand at 251.067 kb on - strand at 251.067 kb on - strand at 251.072 kb on - strand at 251.078 kb on - strand at 251.119 kb on - strand at 251.136 kb on - strand at 251.142 kb on + strand at 251.162 kb on - strand at 251.339 kb on - strand at 251.365 kb on + strand at 251.365 kb on + strand at 251.365 kb on + strand at 251.365 kb on + strand at 251.373 kb on - strand at 251.373 kb on - strand at 251.373 kb on - strand at 251.373 kb on - strand at 251.377 kb on + strand at 251.377 kb on + strand at 251.377 kb on + strand at 251.385 kb on + strand at 251.385 kb on + strand at 251.385 kb on - strand at 251.420 kb on - strand at 251.438 kb on + strand at 251.443 kb on + strand at 251.443 kb on + strand at 251.446 kb on - strand at 251.446 kb on - strand at 251.447 kb on + strand at 251.447 kb on + strand at 251.447 kb on + strand at 251.451 kb on - strand at 251.466 kb on - strand at 251.495 kb on + strand at 251.516 kb on + strand, within BBR_RS11180 at 251.516 kb on + strand, within BBR_RS11180 at 251.521 kb on + strand, within BBR_RS11180 at 251.539 kb on + strand, within BBR_RS11180 at 251.547 kb on - strand, within BBR_RS11180 at 251.556 kb on - strand, within BBR_RS11180 at 251.574 kb on - strand, within BBR_RS11180 at 251.599 kb on + strand, within BBR_RS11180 at 251.604 kb on + strand, within BBR_RS11180 at 251.604 kb on + strand, within BBR_RS11180 at 251.607 kb on + strand, within BBR_RS11180 at 251.618 kb on + strand, within BBR_RS11180 at 251.638 kb on + strand, within BBR_RS11180 at 251.638 kb on + strand, within BBR_RS11180 at 251.638 kb on + strand, within BBR_RS11180 at 251.638 kb on + strand, within BBR_RS11180 at 251.644 kb on + strand, within BBR_RS11180 at 251.646 kb on + strand, within BBR_RS11180 at 251.646 kb on - strand, within BBR_RS11180 at 251.646 kb on - strand, within BBR_RS11180 at 251.646 kb on - strand, within BBR_RS11180 at 251.646 kb on - strand, within BBR_RS11180 at 251.646 kb on - strand, within BBR_RS11180 at 251.646 kb on - strand, within BBR_RS11180 at 251.648 kb on + strand, within BBR_RS11180 at 251.648 kb on + strand, within BBR_RS11180 at 251.648 kb on + strand, within BBR_RS11180 at 251.648 kb on + strand, within BBR_RS11180 at 251.648 kb on + strand, within BBR_RS11180 at 251.648 kb on + strand, within BBR_RS11180 at 251.648 kb on + strand, within BBR_RS11180 at 251.653 kb on + strand, within BBR_RS11180 at 251.653 kb on + strand, within BBR_RS11180 at 251.653 kb on + strand, within BBR_RS11180 at 251.653 kb on + strand, within BBR_RS11180 at 251.654 kb on + strand, within BBR_RS11180 at 251.656 kb on - strand, within BBR_RS11180 at 251.656 kb on - strand, within BBR_RS11180 at 251.656 kb on - strand, within BBR_RS11180 at 251.656 kb on - strand, within BBR_RS11180 at 251.656 kb on - strand, within BBR_RS11180 at 251.661 kb on - strand, within BBR_RS11180 at 251.661 kb on - strand, within BBR_RS11180 at 251.689 kb on + strand, within BBR_RS11180 at 251.689 kb on + strand, within BBR_RS11180 at 251.691 kb on + strand, within BBR_RS11180 at 251.692 kb on - strand, within BBR_RS11180 at 251.697 kb on - strand, within BBR_RS11180 at 251.728 kb on - strand, within BBR_RS11180 at 251.743 kb on + strand, within BBR_RS11180 at 251.751 kb on - strand, within BBR_RS11180 at 251.751 kb on - strand, within BBR_RS11180 at 251.783 kb on + strand, within BBR_RS11180 at 251.805 kb on - strand, within BBR_RS11180 at 251.844 kb on - strand, within BBR_RS11180 at 251.856 kb on + strand, within BBR_RS11180 at 251.874 kb on + strand, within BBR_RS11180 at 251.874 kb on + strand, within BBR_RS11180 at 251.919 kb on + strand, within BBR_RS11180 at 251.938 kb on + strand, within BBR_RS11180 at 251.943 kb on - strand, within BBR_RS11180 at 251.943 kb on - strand, within BBR_RS11180 at 251.969 kb on - strand, within BBR_RS11180 at 251.969 kb on - strand, within BBR_RS11180 at 251.974 kb on - strand, within BBR_RS11180 at 251.987 kb on + strand, within BBR_RS11180 at 251.987 kb on + strand, within BBR_RS11180 at 251.987 kb on + strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.997 kb on + strand, within BBR_RS11180 at 252.017 kb on + strand, within BBR_RS11180 at 252.025 kb on - strand, within BBR_RS11180 at 252.025 kb on - strand, within BBR_RS11180 at 252.025 kb on - strand, within BBR_RS11180 at 252.039 kb on + strand, within BBR_RS11180 at 252.057 kb on + strand, within BBR_RS11180 at 252.057 kb on + strand, within BBR_RS11180 at 252.066 kb on + strand, within BBR_RS11180 at 252.070 kb on - strand, within BBR_RS11180 at 252.074 kb on - strand, within BBR_RS11180 at 252.082 kb on + strand, within BBR_RS11180 at 252.107 kb on + strand, within BBR_RS11180 at 252.109 kb on + strand, within BBR_RS11180 at 252.109 kb on + strand, within BBR_RS11180 at 252.152 kb on + strand, within BBR_RS11180 at 252.160 kb on - strand, within BBR_RS11180 at 252.160 kb on - strand, within BBR_RS11180 at 252.160 kb on - strand, within BBR_RS11180 at 252.205 kb on - strand, within BBR_RS11180 at 252.212 kb on + strand, within BBR_RS11180 at 252.214 kb on + strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.224 kb on + strand, within BBR_RS11180 at 252.234 kb on + strand, within BBR_RS11180 at 252.235 kb on + strand, within BBR_RS11180 at 252.235 kb on + strand, within BBR_RS11180 at 252.243 kb on - strand, within BBR_RS11180 at 252.255 kb on + strand, within BBR_RS11180 at 252.273 kb on + strand, within BBR_RS11180 at 252.274 kb on + strand, within BBR_RS11180 at 252.280 kb on - strand, within BBR_RS11180 at 252.286 kb on - strand, within BBR_RS11180 at 252.287 kb on - strand, within BBR_RS11180 at 252.292 kb on + strand, within BBR_RS11180 at 252.296 kb on + strand, within BBR_RS11180 at 252.298 kb on + strand, within BBR_RS11180 at 252.304 kb on - strand, within BBR_RS11180 at 252.330 kb on + strand, within BBR_RS11180 at 252.330 kb on + strand, within BBR_RS11180 at 252.336 kb on - strand, within BBR_RS11180 at 252.346 kb on + strand, within BBR_RS11180 at 252.356 kb on - strand, within BBR_RS11180 at 252.358 kb on - strand, within BBR_RS11180 at 252.375 kb on - strand, within BBR_RS11180 at 252.375 kb on - strand, within BBR_RS11180 at 252.384 kb on - strand, within BBR_RS11180 at 252.431 kb on - strand, within BBR_RS11180 at 252.432 kb on + strand, within BBR_RS11180 at 252.433 kb on - strand, within BBR_RS11180 at 252.433 kb on - strand, within BBR_RS11180 at 252.447 kb on + strand, within BBR_RS11180 at 252.457 kb on + strand at 252.478 kb on + strand at 252.478 kb on + strand at 252.478 kb on + strand at 252.486 kb on - strand at 252.486 kb on - strand at 252.486 kb on - strand at 252.486 kb on - strand at 252.488 kb on + strand at 252.503 kb on + strand at 252.511 kb on - strand at 252.524 kb on + strand at 252.524 kb on + strand at 252.527 kb on - strand at 252.527 kb on - strand at 252.534 kb on + strand at 252.534 kb on + strand at 252.557 kb on - strand at 252.558 kb on + strand at 252.571 kb on + strand at 252.602 kb on - strand at 252.623 kb on - strand at 252.623 kb on - strand at 252.660 kb on + strand at 252.667 kb on + strand at 252.667 kb on + strand at 252.667 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with WakoIMO-prebiotic 0.5 %w/v remove 250,402 + BBR_RS11175 0.74 +0.2 250,410 - BBR_RS11175 0.74 -1.5 250,413 + BBR_RS11175 0.75 +0.2 250,441 - BBR_RS11175 0.76 -0.6 250,441 - BBR_RS11175 0.76 +0.8 250,441 - BBR_RS11175 0.76 -1.9 250,441 - BBR_RS11175 0.76 -0.1 250,453 + BBR_RS11175 0.76 -1.1 250,453 + BBR_RS11175 0.76 -0.4 250,455 + BBR_RS11175 0.76 -1.5 250,461 - BBR_RS11175 0.77 +1.2 250,465 + BBR_RS11175 0.77 -0.0 250,501 + BBR_RS11175 0.78 -1.6 250,501 + BBR_RS11175 0.78 -1.4 250,501 + BBR_RS11175 0.78 +0.3 250,516 + BBR_RS11175 0.79 +0.6 250,524 - BBR_RS11175 0.79 +0.1 250,524 - BBR_RS11175 0.79 -2.3 250,547 + BBR_RS11175 0.80 -1.1 250,547 + BBR_RS11175 0.80 -0.2 250,547 + BBR_RS11175 0.80 -1.3 250,555 - BBR_RS11175 0.81 -1.3 250,555 - BBR_RS11175 0.81 -1.6 250,647 - BBR_RS11175 0.85 -2.3 250,667 + BBR_RS11175 0.85 -0.9 250,675 - BBR_RS11175 0.86 +0.4 250,727 + BBR_RS11175 0.88 +0.1 250,727 + BBR_RS11175 0.88 -1.7 250,730 + BBR_RS11175 0.88 -1.1 250,738 - BBR_RS11175 0.88 -0.4 250,772 + BBR_RS11175 0.90 -2.2 250,790 - -0.8 250,807 + -0.9 250,807 + -1.7 250,808 - +0.4 250,856 + -0.1 250,856 + -0.5 250,856 + -0.9 250,864 - +1.7 250,864 - -0.8 250,870 - -0.9 250,877 + -1.0 250,931 + +0.2 250,931 + -0.9 250,939 - -1.0 250,939 - -2.7 250,939 - -2.4 250,939 - -1.1 250,939 - -1.6 250,949 - -0.2 250,960 - -1.0 250,960 - -0.7 250,960 - +0.1 250,967 + +0.5 250,969 + -0.9 250,979 + -0.4 250,986 + -0.9 251,016 + +0.2 251,042 + +0.1 251,050 - -0.2 251,057 + +0.4 251,057 + -0.9 251,057 - -0.3 251,057 - -0.1 251,064 + +0.0 251,067 - +0.6 251,067 - -0.5 251,072 - +0.2 251,078 - -0.0 251,119 - +0.0 251,136 - -0.1 251,142 + +0.6 251,162 - -0.9 251,339 - -0.4 251,365 + -0.9 251,365 + -1.0 251,365 + -0.6 251,365 + +1.1 251,373 - -1.4 251,373 - -2.9 251,373 - -0.5 251,373 - -1.2 251,377 + -1.0 251,377 + -1.2 251,377 + +0.2 251,385 + -0.5 251,385 + +0.3 251,385 - -3.0 251,420 - -1.7 251,438 + -2.3 251,443 + -3.0 251,443 + -2.5 251,446 - -3.3 251,446 - -1.1 251,447 + +0.4 251,447 + -1.2 251,447 + -1.0 251,451 - -4.5 251,466 - -2.5 251,495 + -1.7 251,516 + BBR_RS11180 0.10 +0.3 251,516 + BBR_RS11180 0.10 -0.2 251,521 + BBR_RS11180 0.11 -2.7 251,539 + BBR_RS11180 0.12 -3.1 251,547 - BBR_RS11180 0.13 -2.3 251,556 - BBR_RS11180 0.14 -1.9 251,574 - BBR_RS11180 0.15 -1.5 251,599 + BBR_RS11180 0.17 -2.3 251,604 + BBR_RS11180 0.18 -2.7 251,604 + BBR_RS11180 0.18 -2.3 251,607 + BBR_RS11180 0.18 -1.7 251,618 + BBR_RS11180 0.19 -0.7 251,638 + BBR_RS11180 0.21 -2.2 251,638 + BBR_RS11180 0.21 -2.6 251,638 + BBR_RS11180 0.21 -3.7 251,638 + BBR_RS11180 0.21 -1.8 251,644 + BBR_RS11180 0.21 -3.2 251,646 + BBR_RS11180 0.21 -1.8 251,646 - BBR_RS11180 0.21 -3.7 251,646 - BBR_RS11180 0.21 -3.6 251,646 - BBR_RS11180 0.21 -3.7 251,646 - BBR_RS11180 0.21 -3.3 251,646 - BBR_RS11180 0.21 -3.1 251,646 - BBR_RS11180 0.21 -4.1 251,648 + BBR_RS11180 0.22 -1.2 251,648 + BBR_RS11180 0.22 -2.0 251,648 + BBR_RS11180 0.22 -0.4 251,648 + BBR_RS11180 0.22 -1.4 251,648 + BBR_RS11180 0.22 -1.2 251,648 + BBR_RS11180 0.22 -2.1 251,648 + BBR_RS11180 0.22 -0.2 251,653 + BBR_RS11180 0.22 -2.8 251,653 + BBR_RS11180 0.22 -2.7 251,653 + BBR_RS11180 0.22 -3.4 251,653 + BBR_RS11180 0.22 -4.0 251,654 + BBR_RS11180 0.22 -1.0 251,656 - BBR_RS11180 0.22 -1.4 251,656 - BBR_RS11180 0.22 -1.9 251,656 - BBR_RS11180 0.22 -1.8 251,656 - BBR_RS11180 0.22 -0.6 251,656 - BBR_RS11180 0.22 -1.6 251,661 - BBR_RS11180 0.23 -2.2 251,661 - BBR_RS11180 0.23 -2.3 251,689 + BBR_RS11180 0.25 -0.2 251,689 + BBR_RS11180 0.25 -3.3 251,691 + BBR_RS11180 0.25 -3.5 251,692 - BBR_RS11180 0.25 -2.5 251,697 - BBR_RS11180 0.26 -3.7 251,728 - BBR_RS11180 0.28 -1.2 251,743 + BBR_RS11180 0.30 -2.5 251,751 - BBR_RS11180 0.30 -2.5 251,751 - BBR_RS11180 0.30 -3.8 251,783 + BBR_RS11180 0.33 -3.3 251,805 - BBR_RS11180 0.35 -2.9 251,844 - BBR_RS11180 0.38 -3.7 251,856 + BBR_RS11180 0.39 -3.4 251,874 + BBR_RS11180 0.41 -4.0 251,874 + BBR_RS11180 0.41 -4.5 251,919 + BBR_RS11180 0.45 -2.5 251,938 + BBR_RS11180 0.46 -4.0 251,943 - BBR_RS11180 0.47 -4.3 251,943 - BBR_RS11180 0.47 -3.1 251,969 - BBR_RS11180 0.49 -2.4 251,969 - BBR_RS11180 0.49 -3.8 251,974 - BBR_RS11180 0.49 -3.3 251,987 + BBR_RS11180 0.50 -2.9 251,987 + BBR_RS11180 0.50 -4.2 251,987 + BBR_RS11180 0.50 -3.1 251,995 - BBR_RS11180 0.51 -1.4 251,995 - BBR_RS11180 0.51 -2.5 251,995 - BBR_RS11180 0.51 -3.0 251,995 - BBR_RS11180 0.51 -1.4 251,995 - BBR_RS11180 0.51 -3.0 251,995 - BBR_RS11180 0.51 -1.7 251,997 + BBR_RS11180 0.51 -3.2 252,017 + BBR_RS11180 0.53 -1.8 252,025 - BBR_RS11180 0.54 -2.8 252,025 - BBR_RS11180 0.54 -0.7 252,025 - BBR_RS11180 0.54 -2.7 252,039 + BBR_RS11180 0.55 -3.9 252,057 + BBR_RS11180 0.56 -3.8 252,057 + BBR_RS11180 0.56 -3.0 252,066 + BBR_RS11180 0.57 -2.6 252,070 - BBR_RS11180 0.57 -2.9 252,074 - BBR_RS11180 0.58 -3.6 252,082 + BBR_RS11180 0.58 -3.7 252,107 + BBR_RS11180 0.60 -3.5 252,109 + BBR_RS11180 0.61 -1.4 252,109 + BBR_RS11180 0.61 -2.3 252,152 + BBR_RS11180 0.64 -4.9 252,160 - BBR_RS11180 0.65 -0.9 252,160 - BBR_RS11180 0.65 -2.4 252,160 - BBR_RS11180 0.65 -2.5 252,205 - BBR_RS11180 0.69 -2.8 252,212 + BBR_RS11180 0.69 -0.8 252,214 + BBR_RS11180 0.70 -2.9 252,222 - BBR_RS11180 0.70 -2.5 252,222 - BBR_RS11180 0.70 -4.1 252,222 - BBR_RS11180 0.70 -3.6 252,222 - BBR_RS11180 0.70 -1.6 252,224 + BBR_RS11180 0.70 +0.4 252,234 + BBR_RS11180 0.71 -3.2 252,235 + BBR_RS11180 0.71 -2.4 252,235 + BBR_RS11180 0.71 -3.3 252,243 - BBR_RS11180 0.72 -3.5 252,255 + BBR_RS11180 0.73 -3.3 252,273 + BBR_RS11180 0.75 -2.8 252,274 + BBR_RS11180 0.75 -3.2 252,280 - BBR_RS11180 0.75 -4.0 252,286 - BBR_RS11180 0.76 -3.3 252,287 - BBR_RS11180 0.76 -3.3 252,292 + BBR_RS11180 0.76 -4.5 252,296 + BBR_RS11180 0.77 -2.1 252,298 + BBR_RS11180 0.77 -3.9 252,304 - BBR_RS11180 0.77 -2.0 252,330 + BBR_RS11180 0.79 -2.8 252,330 + BBR_RS11180 0.79 -3.5 252,336 - BBR_RS11180 0.80 -2.7 252,346 + BBR_RS11180 0.81 -3.2 252,356 - BBR_RS11180 0.82 -2.1 252,358 - BBR_RS11180 0.82 -1.5 252,375 - BBR_RS11180 0.83 -3.5 252,375 - BBR_RS11180 0.83 -1.5 252,384 - BBR_RS11180 0.84 -1.2 252,431 - BBR_RS11180 0.88 -2.8 252,432 + BBR_RS11180 0.88 -4.3 252,433 - BBR_RS11180 0.88 -2.6 252,433 - BBR_RS11180 0.88 -4.3 252,447 + BBR_RS11180 0.89 -3.9 252,457 + -3.3 252,478 + -2.1 252,478 + -2.8 252,478 + -3.5 252,486 - -3.6 252,486 - -1.3 252,486 - -2.9 252,486 - -2.8 252,488 + -1.8 252,503 + -3.2 252,511 - -4.1 252,524 + -3.4 252,524 + -2.9 252,527 - -3.1 252,527 - -3.6 252,534 + -4.2 252,534 + -2.5 252,557 - -2.6 252,558 + -1.4 252,571 + +0.4 252,602 - -0.4 252,623 - -1.5 252,623 - -0.2 252,660 + +0.1 252,667 + +0.5 252,667 + -2.7 252,667 + -0.7
Or see this region's nucleotide sequence