Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2246 and Dshi_2247 are separated by 61 nucleotides Dshi_2247 and Dshi_2248 are separated by 112 nucleotides Dshi_2248 and Dshi_2249 are separated by 8 nucleotides Dshi_2249 and Dshi_2250 overlap by 4 nucleotides
Dshi_2246: Dshi_2246 - ISSpo6, transposase OrfB (RefSeq), at 2,369,013 to 2,369,543
_2246
Dshi_2247: Dshi_2247 - ISSpo6, transposase orf A (RefSeq), at 2,369,605 to 2,369,967
_2247
Dshi_2248: Dshi_2248 - putative aggregation factor core (RefSeq), at 2,370,080 to 2,370,526
_2248
Dshi_2249: Dshi_2249 - NusB antitermination factor (RefSeq), at 2,370,535 to 2,371,014
_2249
Dshi_2250: Dshi_2250 - 6,7-dimethyl-8-ribityllumazine synthase (RefSeq), at 2,371,011 to 2,371,562
_2250
Position (kb)
2370
2371 Strain fitness (log2 ratio)
-1
0
1 at 2370.085 kb on - strand at 2370.118 kb on + strand at 2370.126 kb on - strand, within Dshi_2248 at 2370.236 kb on - strand, within Dshi_2248 at 2370.238 kb on + strand, within Dshi_2248 at 2370.246 kb on - strand, within Dshi_2248 at 2370.260 kb on - strand, within Dshi_2248 at 2370.292 kb on + strand, within Dshi_2248 at 2370.314 kb on + strand, within Dshi_2248
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 2 mM remove 2,370,085 - +1.1 2,370,118 + -1.1 2,370,126 - Dshi_2248 0.10 +0.3 2,370,236 - Dshi_2248 0.35 -0.0 2,370,238 + Dshi_2248 0.35 +0.5 2,370,246 - Dshi_2248 0.37 -0.3 2,370,260 - Dshi_2248 0.40 +0.9 2,370,292 + Dshi_2248 0.47 +1.2 2,370,314 + Dshi_2248 0.52 +0.7
Or see this region's nucleotide sequence