Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0820 and Dshi_5001 are separated by 81 nucleotides Dshi_5001 and Dshi_0821 are separated by 13 nucleotides Dshi_0821 and Dshi_0822 are separated by 197 nucleotides Dshi_0822 and Dshi_0823 are separated by 4 nucleotides
Dshi_0820: Dshi_0820 - alpha/beta hydrolase fold (RefSeq), at 824,267 to 825,034
_0820
Dshi_5001: Dshi_5001 - hypothtical protein (RefSeq), at 825,116 to 825,505
_5001
Dshi_0821: Dshi_0821 - Glycine hydroxymethyltransferase (RefSeq), at 825,519 to 826,814
_0821
Dshi_0822: Dshi_0822 - NAD(+) kinase (RefSeq), at 827,012 to 827,779
_0822
Dshi_0823: Dshi_0823 - two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq), at 827,784 to 829,175
_0823
Position (kb) 825
826
827 Strain fitness (log2 ratio) -2
-1
0
1
2 at 824.686 kb on + strand, within Dshi_0820 at 824.794 kb on - strand, within Dshi_0820 at 824.876 kb on - strand, within Dshi_0820 at 824.917 kb on + strand, within Dshi_0820 at 824.969 kb on - strand at 825.020 kb on - strand at 825.064 kb on - strand at 825.121 kb on - strand at 825.125 kb on - strand at 825.298 kb on - strand, within Dshi_5001 at 825.309 kb on + strand, within Dshi_5001 at 825.379 kb on + strand, within Dshi_5001 at 825.494 kb on + strand at 825.525 kb on - strand at 825.528 kb on + strand at 825.692 kb on + strand, within Dshi_0821 at 825.713 kb on + strand, within Dshi_0821 at 825.799 kb on - strand, within Dshi_0821 at 825.842 kb on + strand, within Dshi_0821 at 825.850 kb on - strand, within Dshi_0821 at 826.342 kb on - strand, within Dshi_0821 at 826.435 kb on - strand, within Dshi_0821 at 826.610 kb on + strand, within Dshi_0821 at 826.623 kb on - strand, within Dshi_0821 at 826.643 kb on + strand, within Dshi_0821 at 826.646 kb on - strand, within Dshi_0821 at 826.846 kb on - strand at 826.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 2 mM remove 824,686 + Dshi_0820 0.55 -1.4 824,794 - Dshi_0820 0.69 -0.6 824,876 - Dshi_0820 0.79 +0.2 824,917 + Dshi_0820 0.85 -0.5 824,969 - -0.8 825,020 - -0.2 825,064 - -0.5 825,121 - -1.9 825,125 - -0.2 825,298 - Dshi_5001 0.47 +0.9 825,309 + Dshi_5001 0.49 -0.5 825,379 + Dshi_5001 0.67 +1.6 825,494 + -0.2 825,525 - -0.4 825,528 + -0.1 825,692 + Dshi_0821 0.13 -0.8 825,713 + Dshi_0821 0.15 +0.1 825,799 - Dshi_0821 0.22 +1.8 825,842 + Dshi_0821 0.25 -1.4 825,850 - Dshi_0821 0.26 -1.8 826,342 - Dshi_0821 0.64 +0.7 826,435 - Dshi_0821 0.71 +0.6 826,610 + Dshi_0821 0.84 -0.5 826,623 - Dshi_0821 0.85 -0.1 826,643 + Dshi_0821 0.87 -0.3 826,646 - Dshi_0821 0.87 -1.3 826,846 - -0.0 826,847 + +0.5
Or see this region's nucleotide sequence