Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0024 and Dshi_0025 are separated by 323 nucleotides Dshi_0025 and Dshi_0026 are separated by 73 nucleotides Dshi_0026 and Dshi_0027 are separated by 123 nucleotides
Dshi_0024: Dshi_0024 - DNA methylase N-4/N-6 domain protein (RefSeq), at 27,713 to 28,819
_0024
Dshi_0025: Dshi_0025 - anti-ECFsigma factor, ChrR (RefSeq), at 29,143 to 29,814
_0025
Dshi_0026: Dshi_0026 - NAD-dependent epimerase/dehydratase (RefSeq), at 29,888 to 30,529
_0026
Dshi_0027: Dshi_0027 - Alkane 1-monooxygenase (RefSeq), at 30,653 to 31,807
_0027
Position (kb)
29
30 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 28.889 kb on + strand at 28.945 kb on - strand at 28.964 kb on - strand at 29.006 kb on + strand at 29.006 kb on + strand at 29.190 kb on + strand at 29.191 kb on + strand at 29.198 kb on - strand at 29.337 kb on - strand, within Dshi_0025 at 29.390 kb on + strand, within Dshi_0025 at 29.398 kb on - strand, within Dshi_0025 at 29.434 kb on + strand, within Dshi_0025 at 29.436 kb on - strand, within Dshi_0025 at 29.463 kb on - strand, within Dshi_0025 at 29.469 kb on + strand, within Dshi_0025 at 29.575 kb on + strand, within Dshi_0025 at 29.586 kb on + strand, within Dshi_0025 at 29.667 kb on + strand, within Dshi_0025 at 29.723 kb on + strand, within Dshi_0025 at 29.731 kb on - strand at 29.821 kb on - strand at 29.822 kb on - strand at 29.838 kb on - strand at 29.996 kb on + strand, within Dshi_0026 at 30.039 kb on - strand, within Dshi_0026 at 30.258 kb on - strand, within Dshi_0026 at 30.314 kb on + strand, within Dshi_0026 at 30.337 kb on - strand, within Dshi_0026 at 30.341 kb on + strand, within Dshi_0026 at 30.341 kb on + strand, within Dshi_0026 at 30.351 kb on + strand, within Dshi_0026 at 30.395 kb on + strand, within Dshi_0026 at 30.403 kb on - strand, within Dshi_0026 at 30.419 kb on + strand, within Dshi_0026 at 30.703 kb on - strand at 30.756 kb on + strand at 30.780 kb on + strand, within Dshi_0027 at 30.811 kb on + strand, within Dshi_0027
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 2 mM remove 28,889 + +0.5 28,945 - -1.1 28,964 - +0.9 29,006 + +0.2 29,006 + -0.7 29,190 + +0.5 29,191 + -1.0 29,198 - +0.4 29,337 - Dshi_0025 0.29 +0.5 29,390 + Dshi_0025 0.37 +0.6 29,398 - Dshi_0025 0.38 +0.6 29,434 + Dshi_0025 0.43 +0.5 29,436 - Dshi_0025 0.44 -0.1 29,463 - Dshi_0025 0.48 +0.9 29,469 + Dshi_0025 0.49 +0.0 29,575 + Dshi_0025 0.64 +0.9 29,586 + Dshi_0025 0.66 +0.3 29,667 + Dshi_0025 0.78 -2.2 29,723 + Dshi_0025 0.86 +0.7 29,731 - +0.2 29,821 - +0.3 29,822 - -0.7 29,838 - +1.4 29,996 + Dshi_0026 0.17 +0.5 30,039 - Dshi_0026 0.24 +0.7 30,258 - Dshi_0026 0.58 +0.6 30,314 + Dshi_0026 0.66 -0.3 30,337 - Dshi_0026 0.70 -0.1 30,341 + Dshi_0026 0.71 +0.1 30,341 + Dshi_0026 0.71 +0.3 30,351 + Dshi_0026 0.72 -0.3 30,395 + Dshi_0026 0.79 -0.9 30,403 - Dshi_0026 0.80 +0.3 30,419 + Dshi_0026 0.83 -3.0 30,703 - +0.3 30,756 + -1.5 30,780 + Dshi_0027 0.11 -0.6 30,811 + Dshi_0027 0.14 -1.7
Or see this region's nucleotide sequence