Experiment: reconstituted MRS with Avantafiber 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14730 and BBR_RS14735 are separated by 75 nucleotides BBR_RS14735 and BBR_RS20850 overlap by 9 nucleotides BBR_RS20850 and BBR_RS14740 are separated by 179 nucleotides BBR_RS14740 and BBR_RS20855 are separated by 278 nucleotides
BBR_RS14730: BBR_RS14730 - ATPase AAA, at 1,079,341 to 1,080,126
_RS14730
BBR_RS14735: BBR_RS14735 - TetR family transcriptional regulator, at 1,080,202 to 1,080,689
_RS14735
BBR_RS20850: BBR_RS20850 - resolvase, at 1,080,681 to 1,080,923
_RS20850
BBR_RS14740: BBR_RS14740 - YccF domain-containing protein, at 1,081,103 to 1,081,468
_RS14740
BBR_RS20855: BBR_RS20855 - hypothetical protein, at 1,081,747 to 1,082,028
_RS20855
Position (kb)
1080
1081 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1080.264 kb on + strand, within BBR_RS14735 at 1080.264 kb on + strand, within BBR_RS14735 at 1080.298 kb on + strand, within BBR_RS14735 at 1080.298 kb on - strand, within BBR_RS14735 at 1080.316 kb on + strand, within BBR_RS14735 at 1080.349 kb on + strand, within BBR_RS14735 at 1080.384 kb on - strand, within BBR_RS14735 at 1080.453 kb on - strand, within BBR_RS14735 at 1080.461 kb on + strand, within BBR_RS14735 at 1080.505 kb on + strand, within BBR_RS14735 at 1080.513 kb on - strand, within BBR_RS14735 at 1080.559 kb on + strand, within BBR_RS14735 at 1080.559 kb on + strand, within BBR_RS14735 at 1080.559 kb on + strand, within BBR_RS14735 at 1080.559 kb on + strand, within BBR_RS14735 at 1080.567 kb on - strand, within BBR_RS14735 at 1080.567 kb on - strand, within BBR_RS14735 at 1080.567 kb on - strand, within BBR_RS14735 at 1080.567 kb on - strand, within BBR_RS14735 at 1080.567 kb on - strand, within BBR_RS14735 at 1080.569 kb on + strand, within BBR_RS14735 at 1080.569 kb on + strand, within BBR_RS14735 at 1080.569 kb on + strand, within BBR_RS14735 at 1080.569 kb on + strand, within BBR_RS14735 at 1080.577 kb on - strand, within BBR_RS14735 at 1080.577 kb on - strand, within BBR_RS14735 at 1080.577 kb on - strand, within BBR_RS14735 at 1080.577 kb on - strand, within BBR_RS14735 at 1080.596 kb on - strand, within BBR_RS14735 at 1080.674 kb on + strand at 1080.864 kb on - strand, within BBR_RS20850 at 1080.901 kb on + strand at 1080.905 kb on + strand at 1080.914 kb on + strand at 1080.927 kb on - strand at 1080.932 kb on - strand at 1080.982 kb on - strand at 1081.092 kb on - strand at 1081.189 kb on + strand, within BBR_RS14740 at 1081.223 kb on + strand, within BBR_RS14740 at 1081.231 kb on - strand, within BBR_RS14740 at 1081.268 kb on + strand, within BBR_RS14740 at 1081.271 kb on - strand, within BBR_RS14740 at 1081.276 kb on - strand, within BBR_RS14740 at 1081.348 kb on + strand, within BBR_RS14740 at 1081.348 kb on + strand, within BBR_RS14740 at 1081.395 kb on - strand, within BBR_RS14740 at 1081.421 kb on + strand, within BBR_RS14740 at 1081.429 kb on - strand, within BBR_RS14740 at 1081.544 kb on + strand at 1081.558 kb on - strand at 1081.564 kb on - strand at 1081.609 kb on + strand at 1081.661 kb on - strand at 1081.731 kb on + strand at 1081.739 kb on + strand at 1081.739 kb on + strand at 1081.768 kb on + strand at 1081.776 kb on - strand, within BBR_RS20855 at 1081.791 kb on + strand, within BBR_RS20855 at 1081.846 kb on - strand, within BBR_RS20855 at 1081.865 kb on + strand, within BBR_RS20855 at 1081.871 kb on - strand, within BBR_RS20855 at 1081.916 kb on + strand, within BBR_RS20855
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Avantafiber 0.5 %w/v remove 1,080,264 + BBR_RS14735 0.13 -0.8 1,080,264 + BBR_RS14735 0.13 +0.7 1,080,298 + BBR_RS14735 0.20 +2.6 1,080,298 - BBR_RS14735 0.20 +0.2 1,080,316 + BBR_RS14735 0.23 +0.6 1,080,349 + BBR_RS14735 0.30 -0.8 1,080,384 - BBR_RS14735 0.37 +0.7 1,080,453 - BBR_RS14735 0.51 +0.5 1,080,461 + BBR_RS14735 0.53 -1.4 1,080,505 + BBR_RS14735 0.62 -0.8 1,080,513 - BBR_RS14735 0.64 +0.5 1,080,559 + BBR_RS14735 0.73 +0.2 1,080,559 + BBR_RS14735 0.73 -0.9 1,080,559 + BBR_RS14735 0.73 +0.0 1,080,559 + BBR_RS14735 0.73 +0.4 1,080,567 - BBR_RS14735 0.75 +0.8 1,080,567 - BBR_RS14735 0.75 -0.6 1,080,567 - BBR_RS14735 0.75 -0.1 1,080,567 - BBR_RS14735 0.75 -0.8 1,080,567 - BBR_RS14735 0.75 +0.2 1,080,569 + BBR_RS14735 0.75 -0.2 1,080,569 + BBR_RS14735 0.75 -0.6 1,080,569 + BBR_RS14735 0.75 +0.4 1,080,569 + BBR_RS14735 0.75 -0.3 1,080,577 - BBR_RS14735 0.77 -1.3 1,080,577 - BBR_RS14735 0.77 -0.8 1,080,577 - BBR_RS14735 0.77 -0.4 1,080,577 - BBR_RS14735 0.77 +0.1 1,080,596 - BBR_RS14735 0.81 -0.4 1,080,674 + +0.5 1,080,864 - BBR_RS20850 0.75 -2.9 1,080,901 + -0.6 1,080,905 + -1.3 1,080,914 + +0.2 1,080,927 - +0.7 1,080,932 - -0.9 1,080,982 - -0.8 1,081,092 - -0.4 1,081,189 + BBR_RS14740 0.23 +0.0 1,081,223 + BBR_RS14740 0.33 -0.0 1,081,231 - BBR_RS14740 0.35 +0.4 1,081,268 + BBR_RS14740 0.45 -0.0 1,081,271 - BBR_RS14740 0.46 -0.7 1,081,276 - BBR_RS14740 0.47 +0.7 1,081,348 + BBR_RS14740 0.67 -0.3 1,081,348 + BBR_RS14740 0.67 +0.4 1,081,395 - BBR_RS14740 0.80 +0.1 1,081,421 + BBR_RS14740 0.87 -1.4 1,081,429 - BBR_RS14740 0.89 +0.5 1,081,544 + +0.5 1,081,558 - +1.2 1,081,564 - -0.6 1,081,609 + +0.5 1,081,661 - -0.4 1,081,731 + +0.2 1,081,739 + +0.5 1,081,739 + +0.5 1,081,768 + +1.0 1,081,776 - BBR_RS20855 0.10 +0.3 1,081,791 + BBR_RS20855 0.16 +0.1 1,081,846 - BBR_RS20855 0.35 +0.2 1,081,865 + BBR_RS20855 0.42 -0.4 1,081,871 - BBR_RS20855 0.44 -0.9 1,081,916 + BBR_RS20855 0.60 +1.0
Or see this region's nucleotide sequence