Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22665

Experiment: L-Phenylalanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22655 and BWI76_RS22660 overlap by 7 nucleotidesBWI76_RS22660 and BWI76_RS22665 overlap by 20 nucleotidesBWI76_RS22665 and BWI76_RS22670 overlap by 4 nucleotidesBWI76_RS22670 and BWI76_RS22675 overlap by 1 nucleotides BWI76_RS22655: BWI76_RS22655 - protein-L-isoaspartate O-methyltransferase, at 4,722,495 to 4,723,121 _RS22655 BWI76_RS22660: BWI76_RS22660 - 5'/3'-nucleotidase SurE, at 4,723,115 to 4,723,876 _RS22660 BWI76_RS22665: BWI76_RS22665 - tRNA pseudouridine(13) synthase TruD, at 4,723,857 to 4,724,906 _RS22665 BWI76_RS22670: BWI76_RS22670 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 4,724,903 to 4,725,382 _RS22670 BWI76_RS22675: BWI76_RS22675 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 4,725,382 to 4,726,092 _RS22675 Position (kb) 4723 4724 4725Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4722.901 kb on - strand, within BWI76_RS22655at 4722.933 kb on + strand, within BWI76_RS22655at 4722.941 kb on - strandat 4723.281 kb on + strand, within BWI76_RS22660at 4723.289 kb on - strand, within BWI76_RS22660at 4723.368 kb on + strand, within BWI76_RS22660at 4723.376 kb on - strand, within BWI76_RS22660at 4723.376 kb on - strand, within BWI76_RS22660at 4723.376 kb on - strand, within BWI76_RS22660at 4723.463 kb on + strand, within BWI76_RS22660at 4723.637 kb on + strand, within BWI76_RS22660at 4723.657 kb on + strand, within BWI76_RS22660at 4723.665 kb on - strand, within BWI76_RS22660at 4723.685 kb on - strand, within BWI76_RS22660at 4723.831 kb on - strandat 4724.108 kb on - strand, within BWI76_RS22665at 4724.504 kb on + strand, within BWI76_RS22665at 4724.504 kb on + strand, within BWI76_RS22665at 4724.512 kb on - strand, within BWI76_RS22665at 4724.614 kb on - strand, within BWI76_RS22665at 4724.841 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Phenylalanine (N)
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4,722,901 - BWI76_RS22655 0.65 -0.3
4,722,933 + BWI76_RS22655 0.70 -2.5
4,722,941 - +0.0
4,723,281 + BWI76_RS22660 0.22 +1.4
4,723,289 - BWI76_RS22660 0.23 -0.1
4,723,368 + BWI76_RS22660 0.33 -0.2
4,723,376 - BWI76_RS22660 0.34 +0.7
4,723,376 - BWI76_RS22660 0.34 +3.1
4,723,376 - BWI76_RS22660 0.34 +0.1
4,723,463 + BWI76_RS22660 0.46 +0.5
4,723,637 + BWI76_RS22660 0.69 -0.2
4,723,657 + BWI76_RS22660 0.71 +0.6
4,723,665 - BWI76_RS22660 0.72 +0.1
4,723,685 - BWI76_RS22660 0.75 +0.6
4,723,831 - -2.6
4,724,108 - BWI76_RS22665 0.24 -1.5
4,724,504 + BWI76_RS22665 0.62 -1.6
4,724,504 + BWI76_RS22665 0.62 -1.1
4,724,512 - BWI76_RS22665 0.62 +0.5
4,724,614 - BWI76_RS22665 0.72 +0.5
4,724,841 + -0.3

Or see this region's nucleotide sequence