Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22420 and BWI76_RS22425 are separated by 177 nucleotides BWI76_RS22425 and BWI76_RS22430 overlap by 4 nucleotides BWI76_RS22430 and BWI76_RS22435 overlap by 4 nucleotides
BWI76_RS22420: BWI76_RS22420 - LacI family transcriptional regulator, at 4,675,019 to 4,675,981
_RS22420
BWI76_RS22425: BWI76_RS22425 - metal ABC transporter substrate-binding protein, at 4,676,159 to 4,677,076
_RS22425
BWI76_RS22430: BWI76_RS22430 - manganese/iron transporter ATP-binding protein, at 4,677,073 to 4,677,894
_RS22430
BWI76_RS22435: BWI76_RS22435 - iron ABC transporter permease AfeC, at 4,677,891 to 4,678,739
_RS22435
Position (kb) 4676
4677
4678 Strain fitness (log2 ratio) -1
0
1
2 at 4675.258 kb on + strand, within BWI76_RS22420 at 4676.162 kb on - strand at 4676.162 kb on - strand at 4676.183 kb on + strand at 4676.191 kb on - strand at 4676.220 kb on - strand at 4676.463 kb on - strand, within BWI76_RS22425 at 4676.521 kb on + strand, within BWI76_RS22425 at 4676.646 kb on - strand, within BWI76_RS22425 at 4676.724 kb on - strand, within BWI76_RS22425 at 4676.764 kb on - strand, within BWI76_RS22425 at 4676.816 kb on - strand, within BWI76_RS22425 at 4677.107 kb on + strand at 4677.363 kb on + strand, within BWI76_RS22430 at 4677.648 kb on + strand, within BWI76_RS22430 at 4677.811 kb on + strand, within BWI76_RS22430 at 4677.819 kb on - strand at 4678.036 kb on + strand, within BWI76_RS22435
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 4,675,258 + BWI76_RS22420 0.25 -0.8 4,676,162 - +0.7 4,676,162 - -0.0 4,676,183 + +0.2 4,676,191 - +0.7 4,676,220 - +0.6 4,676,463 - BWI76_RS22425 0.33 +2.2 4,676,521 + BWI76_RS22425 0.39 +2.3 4,676,646 - BWI76_RS22425 0.53 +0.3 4,676,724 - BWI76_RS22425 0.62 +0.3 4,676,764 - BWI76_RS22425 0.66 +0.4 4,676,816 - BWI76_RS22425 0.72 +0.7 4,677,107 + +1.8 4,677,363 + BWI76_RS22430 0.35 +1.3 4,677,648 + BWI76_RS22430 0.70 +1.0 4,677,811 + BWI76_RS22430 0.90 -1.5 4,677,819 - -0.2 4,678,036 + BWI76_RS22435 0.17 -0.1
Or see this region's nucleotide sequence