Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21880

Experiment: L-Phenylalanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21875 and BWI76_RS21880 are separated by 116 nucleotidesBWI76_RS21880 and BWI76_RS21885 are separated by 10 nucleotidesBWI76_RS21885 and BWI76_RS21890 are separated by 12 nucleotides BWI76_RS21875: BWI76_RS21875 - LysR family transcriptional regulator, at 4,571,241 to 4,572,152 _RS21875 BWI76_RS21880: BWI76_RS21880 - agmatinase, at 4,572,269 to 4,573,219 _RS21880 BWI76_RS21885: BWI76_RS21885 - AP endonuclease, family 2, at 4,573,230 to 4,574,078 _RS21885 BWI76_RS21890: BWI76_RS21890 - oxidoreductase, at 4,574,091 to 4,575,167 _RS21890 Position (kb) 4572 4573 4574Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4571.274 kb on - strandat 4571.274 kb on - strandat 4571.275 kb on - strandat 4571.277 kb on - strandat 4571.293 kb on + strandat 4571.294 kb on + strandat 4571.303 kb on + strandat 4571.309 kb on + strandat 4571.310 kb on - strandat 4571.311 kb on - strandat 4571.311 kb on - strandat 4571.317 kb on - strandat 4571.334 kb on - strand, within BWI76_RS21875at 4571.396 kb on + strand, within BWI76_RS21875at 4571.401 kb on + strand, within BWI76_RS21875at 4571.407 kb on + strand, within BWI76_RS21875at 4571.453 kb on + strand, within BWI76_RS21875at 4571.460 kb on - strand, within BWI76_RS21875at 4571.491 kb on + strand, within BWI76_RS21875at 4571.562 kb on - strand, within BWI76_RS21875at 4571.619 kb on + strand, within BWI76_RS21875at 4571.626 kb on + strand, within BWI76_RS21875at 4571.626 kb on + strand, within BWI76_RS21875at 4571.634 kb on - strand, within BWI76_RS21875at 4571.875 kb on + strand, within BWI76_RS21875at 4571.974 kb on - strand, within BWI76_RS21875at 4571.974 kb on - strand, within BWI76_RS21875at 4572.084 kb on + strandat 4572.552 kb on + strand, within BWI76_RS21880at 4572.562 kb on + strandat 4572.703 kb on + strand, within BWI76_RS21880at 4572.841 kb on + strand, within BWI76_RS21880at 4572.841 kb on + strand, within BWI76_RS21880at 4572.849 kb on - strand, within BWI76_RS21880at 4572.849 kb on - strand, within BWI76_RS21880at 4572.892 kb on + strand, within BWI76_RS21880at 4572.900 kb on - strand, within BWI76_RS21880at 4572.900 kb on - strandat 4572.955 kb on + strand, within BWI76_RS21880at 4573.006 kb on + strand, within BWI76_RS21880at 4573.261 kb on - strandat 4573.264 kb on + strandat 4573.431 kb on - strand, within BWI76_RS21885at 4573.513 kb on + strand, within BWI76_RS21885at 4573.615 kb on + strand, within BWI76_RS21885at 4573.791 kb on + strand, within BWI76_RS21885at 4573.799 kb on - strand, within BWI76_RS21885at 4573.799 kb on - strand, within BWI76_RS21885at 4573.890 kb on - strand, within BWI76_RS21885at 4574.040 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Phenylalanine (N)
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4,571,274 - +0.2
4,571,274 - -1.7
4,571,275 - +0.5
4,571,277 - -0.3
4,571,293 + -1.1
4,571,294 + -0.5
4,571,303 + -0.7
4,571,309 + +2.4
4,571,310 - -0.8
4,571,311 - -1.0
4,571,311 - +1.6
4,571,317 - +0.9
4,571,334 - BWI76_RS21875 0.10 -1.5
4,571,396 + BWI76_RS21875 0.17 -2.4
4,571,401 + BWI76_RS21875 0.18 -0.8
4,571,407 + BWI76_RS21875 0.18 +0.1
4,571,453 + BWI76_RS21875 0.23 -0.3
4,571,460 - BWI76_RS21875 0.24 -3.4
4,571,491 + BWI76_RS21875 0.27 -1.0
4,571,562 - BWI76_RS21875 0.35 -0.4
4,571,619 + BWI76_RS21875 0.41 +0.4
4,571,626 + BWI76_RS21875 0.42 +0.3
4,571,626 + BWI76_RS21875 0.42 -1.2
4,571,634 - BWI76_RS21875 0.43 +1.1
4,571,875 + BWI76_RS21875 0.70 -0.7
4,571,974 - BWI76_RS21875 0.80 -1.4
4,571,974 - BWI76_RS21875 0.80 +0.8
4,572,084 + -1.1
4,572,552 + BWI76_RS21880 0.30 +0.1
4,572,562 + +0.8
4,572,703 + BWI76_RS21880 0.46 -0.7
4,572,841 + BWI76_RS21880 0.60 +3.5
4,572,841 + BWI76_RS21880 0.60 -1.0
4,572,849 - BWI76_RS21880 0.61 +0.8
4,572,849 - BWI76_RS21880 0.61 +0.4
4,572,892 + BWI76_RS21880 0.66 -0.1
4,572,900 - BWI76_RS21880 0.66 -0.8
4,572,900 - +2.9
4,572,955 + BWI76_RS21880 0.72 -0.7
4,573,006 + BWI76_RS21880 0.77 -2.4
4,573,261 - -0.1
4,573,264 + -0.8
4,573,431 - BWI76_RS21885 0.24 +0.4
4,573,513 + BWI76_RS21885 0.33 +1.4
4,573,615 + BWI76_RS21885 0.45 +0.5
4,573,791 + BWI76_RS21885 0.66 -1.0
4,573,799 - BWI76_RS21885 0.67 +0.7
4,573,799 - BWI76_RS21885 0.67 +0.4
4,573,890 - BWI76_RS21885 0.78 +0.4
4,574,040 - +0.2

Or see this region's nucleotide sequence