Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21115 and BWI76_RS21120 are separated by 32 nucleotides BWI76_RS21120 and BWI76_RS21125 are separated by 96 nucleotides BWI76_RS21125 and BWI76_RS21130 are separated by 107 nucleotides BWI76_RS21130 and BWI76_RS21135 are separated by 1 nucleotides BWI76_RS21135 and BWI76_RS21140 are separated by 1 nucleotides
BWI76_RS21115: BWI76_RS21115 - 3-mercaptopyruvate sulfurtransferase, at 4,423,161 to 4,424,006
_RS21115
BWI76_RS21120: BWI76_RS21120 - enhanced serine sensitivity protein SseB, at 4,424,039 to 4,424,815
_RS21120
BWI76_RS21125: BWI76_RS21125 - aminopeptidase PepB, at 4,424,912 to 4,426,198
_RS21125
BWI76_RS21130: BWI76_RS21130 - Fe-S assembly protein IscX, at 4,426,306 to 4,426,506
_RS21130
BWI76_RS21135: BWI76_RS21135 - ferredoxin, 2Fe-2S type, ISC system, at 4,426,508 to 4,426,843
_RS21135
BWI76_RS21140: BWI76_RS21140 - molecular chaperone HscA, at 4,426,845 to 4,428,695
_RS21140
Position (kb)
4424
4425
4426
4427 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4423.980 kb on + strand at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.290 kb on - strand, within BWI76_RS21120 at 4424.290 kb on - strand at 4424.292 kb on - strand, within BWI76_RS21120 at 4424.384 kb on - strand, within BWI76_RS21120 at 4424.529 kb on + strand, within BWI76_RS21120 at 4424.681 kb on - strand, within BWI76_RS21120 at 4425.034 kb on - strand at 4425.226 kb on + strand, within BWI76_RS21125 at 4425.252 kb on + strand, within BWI76_RS21125 at 4425.260 kb on - strand, within BWI76_RS21125 at 4425.260 kb on - strand, within BWI76_RS21125 at 4425.315 kb on - strand, within BWI76_RS21125 at 4425.357 kb on - strand, within BWI76_RS21125 at 4425.734 kb on - strand, within BWI76_RS21125 at 4425.826 kb on + strand, within BWI76_RS21125 at 4425.899 kb on - strand, within BWI76_RS21125 at 4425.952 kb on - strand, within BWI76_RS21125 at 4426.228 kb on + strand at 4426.228 kb on + strand at 4426.228 kb on + strand at 4426.236 kb on - strand at 4426.236 kb on - strand at 4426.405 kb on - strand, within BWI76_RS21130 at 4426.454 kb on + strand, within BWI76_RS21130 at 4426.459 kb on + strand at 4426.490 kb on + strand at 4427.115 kb on - strand, within BWI76_RS21140
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 4,423,980 + +0.1 4,424,282 + BWI76_RS21120 0.31 -1.1 4,424,282 + BWI76_RS21120 0.31 -1.5 4,424,282 + BWI76_RS21120 0.31 -2.1 4,424,290 - BWI76_RS21120 0.32 +1.9 4,424,290 - +4.4 4,424,292 - BWI76_RS21120 0.33 -0.6 4,424,384 - BWI76_RS21120 0.44 +1.3 4,424,529 + BWI76_RS21120 0.63 -0.1 4,424,681 - BWI76_RS21120 0.83 -0.2 4,425,034 - +1.3 4,425,226 + BWI76_RS21125 0.24 -0.3 4,425,252 + BWI76_RS21125 0.26 +0.6 4,425,260 - BWI76_RS21125 0.27 +1.8 4,425,260 - BWI76_RS21125 0.27 +1.3 4,425,315 - BWI76_RS21125 0.31 -0.9 4,425,357 - BWI76_RS21125 0.35 -1.3 4,425,734 - BWI76_RS21125 0.64 +0.2 4,425,826 + BWI76_RS21125 0.71 -0.0 4,425,899 - BWI76_RS21125 0.77 -0.1 4,425,952 - BWI76_RS21125 0.81 +0.5 4,426,228 + +0.1 4,426,228 + +1.0 4,426,228 + +0.5 4,426,236 - -0.9 4,426,236 - +0.7 4,426,405 - BWI76_RS21130 0.49 -2.7 4,426,454 + BWI76_RS21130 0.74 -1.2 4,426,459 + +0.1 4,426,490 + -0.0 4,427,115 - BWI76_RS21140 0.15 +1.0
Or see this region's nucleotide sequence