Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13140

Experiment: L-Phenylalanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13135 and BWI76_RS13140 are separated by 28 nucleotidesBWI76_RS13140 and BWI76_RS13145 are separated by 262 nucleotides BWI76_RS13135: BWI76_RS13135 - 3-oxoadipyl-CoA thiolase, at 2,738,761 to 2,739,966 _RS13135 BWI76_RS13140: BWI76_RS13140 - phenylacetate-CoA ligase, at 2,739,995 to 2,741,311 _RS13140 BWI76_RS13145: BWI76_RS13145 - phenylacetic acid degradation operon negative regulatory protein PaaX, at 2,741,574 to 2,742,509 _RS13145 Position (kb) 2739 2740 2741 2742Strain fitness (log2 ratio) -1 0 1at 2739.311 kb on - strandat 2739.452 kb on + strand, within BWI76_RS13135at 2739.460 kb on - strand, within BWI76_RS13135at 2739.928 kb on - strandat 2739.931 kb on + strandat 2739.931 kb on + strandat 2740.433 kb on + strand, within BWI76_RS13140at 2740.799 kb on + strand, within BWI76_RS13140at 2740.865 kb on + strand, within BWI76_RS13140at 2741.217 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Phenylalanine (N)
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2,739,311 - -0.6
2,739,452 + BWI76_RS13135 0.57 -0.5
2,739,460 - BWI76_RS13135 0.58 -0.1
2,739,928 - +0.2
2,739,931 + +0.2
2,739,931 + +0.5
2,740,433 + BWI76_RS13140 0.33 -0.8
2,740,799 + BWI76_RS13140 0.61 -0.1
2,740,865 + BWI76_RS13140 0.66 -1.3
2,741,217 - -0.2

Or see this region's nucleotide sequence