Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04175 and BWI76_RS04180 overlap by 60 nucleotides BWI76_RS04180 and BWI76_RS04185 are separated by 125 nucleotides BWI76_RS04185 and BWI76_RS04190 are separated by 117 nucleotides BWI76_RS04190 and BWI76_RS04195 overlap by 20 nucleotides
BWI76_RS04175: BWI76_RS04175 - energy-dependent translational throttle protein EttA, at 859,388 to 861,055
_RS04175
BWI76_RS04180: BWI76_RS04180 - hypothetical protein, at 860,996 to 861,203
_RS04180
BWI76_RS04185: BWI76_RS04185 - murein transglycosylase, at 861,329 to 863,266
_RS04185
BWI76_RS04190: BWI76_RS04190 - Trp operon repressor, at 863,384 to 863,716
_RS04190
BWI76_RS04195: BWI76_RS04195 - non-canonical purine NTP phosphatase, at 863,697 to 864,218
_RS04195
Position (kb)
861
862
863
864 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 861.021 kb on - strand at 861.062 kb on - strand, within BWI76_RS04180 at 861.082 kb on + strand, within BWI76_RS04180 at 861.118 kb on + strand, within BWI76_RS04180 at 861.126 kb on - strand, within BWI76_RS04180 at 861.131 kb on - strand, within BWI76_RS04180 at 861.133 kb on + strand, within BWI76_RS04180 at 861.176 kb on + strand, within BWI76_RS04180 at 861.293 kb on + strand at 861.530 kb on - strand, within BWI76_RS04185 at 861.687 kb on + strand, within BWI76_RS04185 at 861.768 kb on - strand, within BWI76_RS04185 at 861.778 kb on - strand, within BWI76_RS04185 at 861.835 kb on + strand, within BWI76_RS04185 at 861.897 kb on - strand, within BWI76_RS04185 at 862.016 kb on + strand, within BWI76_RS04185 at 862.085 kb on + strand, within BWI76_RS04185 at 862.128 kb on - strand, within BWI76_RS04185 at 862.161 kb on + strand, within BWI76_RS04185 at 862.182 kb on - strand, within BWI76_RS04185 at 862.458 kb on + strand, within BWI76_RS04185 at 862.495 kb on + strand, within BWI76_RS04185 at 862.695 kb on - strand, within BWI76_RS04185 at 863.062 kb on - strand, within BWI76_RS04185 at 863.231 kb on + strand at 863.241 kb on - strand at 863.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 861,021 - -2.9 861,062 - BWI76_RS04180 0.32 +0.6 861,082 + BWI76_RS04180 0.41 +0.2 861,118 + BWI76_RS04180 0.59 -1.3 861,126 - BWI76_RS04180 0.62 +0.6 861,131 - BWI76_RS04180 0.65 +0.7 861,133 + BWI76_RS04180 0.66 -1.2 861,176 + BWI76_RS04180 0.87 +2.2 861,293 + +1.6 861,530 - BWI76_RS04185 0.10 +1.2 861,687 + BWI76_RS04185 0.18 +0.6 861,768 - BWI76_RS04185 0.23 -1.0 861,778 - BWI76_RS04185 0.23 +0.4 861,835 + BWI76_RS04185 0.26 -0.1 861,897 - BWI76_RS04185 0.29 -1.3 862,016 + BWI76_RS04185 0.35 +0.8 862,085 + BWI76_RS04185 0.39 +0.7 862,128 - BWI76_RS04185 0.41 +0.7 862,161 + BWI76_RS04185 0.43 +1.0 862,182 - BWI76_RS04185 0.44 +0.1 862,458 + BWI76_RS04185 0.58 +0.9 862,495 + BWI76_RS04185 0.60 +1.2 862,695 - BWI76_RS04185 0.70 +0.4 863,062 - BWI76_RS04185 0.89 +0.1 863,231 + -0.7 863,241 - -1.0 863,283 - -0.4
Or see this region's nucleotide sequence