Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01555 and BWI76_RS01560 are separated by 253 nucleotides BWI76_RS01560 and BWI76_RS01565 are separated by 95 nucleotides BWI76_RS01565 and BWI76_RS01570 are separated by 40 nucleotides
BWI76_RS01555: BWI76_RS01555 - phosphomethylpyrimidine synthase ThiC, at 316,023 to 317,918
_RS01555
BWI76_RS01560: BWI76_RS01560 - Rsd/AlgQ family anti-sigma factor, at 318,172 to 318,669
_RS01560
BWI76_RS01565: BWI76_RS01565 - NADH pyrophosphatase, at 318,765 to 319,538
_RS01565
BWI76_RS01570: BWI76_RS01570 - uroporphyrinogen decarboxylase, at 319,579 to 320,643
_RS01570
Position (kb) 318
319
320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 317.813 kb on + strand at 317.849 kb on + strand at 317.946 kb on - strand at 317.990 kb on - strand at 318.007 kb on + strand at 318.040 kb on - strand at 318.040 kb on - strand at 318.142 kb on + strand at 318.176 kb on - strand at 318.199 kb on + strand at 318.215 kb on - strand at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.483 kb on - strand, within BWI76_RS01560 at 318.564 kb on + strand, within BWI76_RS01560 at 318.605 kb on - strand, within BWI76_RS01560 at 318.630 kb on + strand at 318.638 kb on - strand at 318.668 kb on - strand at 318.754 kb on + strand at 319.333 kb on + strand, within BWI76_RS01565 at 319.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 317,813 + -0.4 317,849 + +0.2 317,946 - -1.5 317,990 - -0.5 318,007 + -0.4 318,040 - +0.5 318,040 - -0.1 318,142 + -3.0 318,176 - +0.0 318,199 + +0.5 318,215 - -0.2 318,371 + BWI76_RS01560 0.40 +0.5 318,371 + BWI76_RS01560 0.40 -0.2 318,371 + BWI76_RS01560 0.40 -0.8 318,379 - BWI76_RS01560 0.42 -1.3 318,379 - BWI76_RS01560 0.42 -1.0 318,483 - BWI76_RS01560 0.62 -0.8 318,564 + BWI76_RS01560 0.79 -0.1 318,605 - BWI76_RS01560 0.87 +1.3 318,630 + -0.1 318,638 - +0.9 318,668 - -1.0 318,754 + +0.4 319,333 + BWI76_RS01565 0.73 +1.2 319,548 - +1.9
Or see this region's nucleotide sequence