Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00320

Experiment: L-Phenylalanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00315 and BWI76_RS00320 are separated by 58 nucleotidesBWI76_RS00320 and BWI76_RS00325 are separated by 124 nucleotidesBWI76_RS00325 and BWI76_RS00330 overlap by 4 nucleotides BWI76_RS00315: BWI76_RS00315 - 23S ribosomal RNA, at 66,486 to 69,413 _RS00315 BWI76_RS00320: BWI76_RS00320 - 5S ribosomal RNA, at 69,472 to 69,587 _RS00320 BWI76_RS00325: BWI76_RS00325 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 69,712 to 70,218 _RS00325 BWI76_RS00330: BWI76_RS00330 - molybdenum cofactor guanylyltransferase, at 70,215 to 70,796 _RS00330 Position (kb) 69 70Strain fitness (log2 ratio) -1 0 1at 69.863 kb on - strand, within BWI76_RS00325at 69.926 kb on - strand, within BWI76_RS00325at 70.056 kb on + strand, within BWI76_RS00325at 70.124 kb on - strand, within BWI76_RS00325at 70.364 kb on - strand, within BWI76_RS00330at 70.386 kb on + strand, within BWI76_RS00330at 70.525 kb on + strand, within BWI76_RS00330

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Phenylalanine (N)
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69,863 - BWI76_RS00325 0.30 +0.2
69,926 - BWI76_RS00325 0.42 -0.8
70,056 + BWI76_RS00325 0.68 +0.3
70,124 - BWI76_RS00325 0.81 +0.2
70,364 - BWI76_RS00330 0.26 +0.9
70,386 + BWI76_RS00330 0.29 +0.4
70,525 + BWI76_RS00330 0.53 +0.8

Or see this region's nucleotide sequence