Experiment: m.b. 1-ethyl-3-methylimidazolium lysine 0.75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2459 and Dshi_2460 are separated by 17 nucleotides Dshi_2460 and Dshi_2461 are separated by 4 nucleotides Dshi_2461 and Dshi_2462 are separated by 10 nucleotides Dshi_2462 and Dshi_2463 overlap by 8 nucleotides Dshi_2463 and Dshi_2464 are separated by 217 nucleotides
Dshi_2459: Dshi_2459 - hypothetical protein (RefSeq), at 2,608,289 to 2,612,149
_2459
Dshi_2460: Dshi_2460 - GPW/gp25 family protein (RefSeq), at 2,612,167 to 2,612,616
_2460
Dshi_2461: Dshi_2461 - PAAR repeat-containing protein (RefSeq), at 2,612,621 to 2,612,920
_2461
Dshi_2462: Dshi_2462 - Rhs element Vgr protein (RefSeq), at 2,612,931 to 2,614,718
_2462
Dshi_2463: Dshi_2463 - hypothetical protein (RefSeq), at 2,614,711 to 2,615,373
_2463
Dshi_2464: Dshi_2464 - conserved hypothetical phage tail protein (RefSeq), at 2,615,591 to 2,616,073
_2464
Position (kb)
2612
2613
2614
2615 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2611.953 kb on + strand at 2612.164 kb on + strand at 2612.165 kb on + strand at 2612.201 kb on - strand at 2612.207 kb on + strand at 2612.340 kb on - strand, within Dshi_2460 at 2612.403 kb on - strand, within Dshi_2460 at 2612.437 kb on - strand, within Dshi_2460 at 2612.523 kb on + strand, within Dshi_2460 at 2612.536 kb on + strand, within Dshi_2460 at 2612.544 kb on - strand, within Dshi_2460 at 2612.584 kb on - strand at 2612.730 kb on + strand, within Dshi_2461 at 2612.865 kb on + strand, within Dshi_2461 at 2612.870 kb on + strand, within Dshi_2461 at 2612.871 kb on - strand, within Dshi_2461 at 2612.871 kb on - strand, within Dshi_2461 at 2612.886 kb on + strand, within Dshi_2461 at 2612.894 kb on - strand at 2612.910 kb on + strand at 2612.967 kb on - strand at 2613.039 kb on + strand at 2613.069 kb on + strand at 2613.076 kb on + strand at 2613.091 kb on - strand at 2613.121 kb on - strand, within Dshi_2462 at 2613.151 kb on - strand, within Dshi_2462 at 2613.169 kb on - strand, within Dshi_2462 at 2613.202 kb on - strand, within Dshi_2462 at 2613.267 kb on - strand, within Dshi_2462 at 2613.344 kb on + strand, within Dshi_2462 at 2613.386 kb on + strand, within Dshi_2462 at 2613.396 kb on + strand, within Dshi_2462 at 2613.425 kb on - strand, within Dshi_2462 at 2613.538 kb on + strand, within Dshi_2462 at 2613.582 kb on - strand, within Dshi_2462 at 2613.610 kb on - strand, within Dshi_2462 at 2613.695 kb on + strand, within Dshi_2462 at 2613.737 kb on - strand, within Dshi_2462 at 2613.738 kb on + strand, within Dshi_2462 at 2613.738 kb on - strand, within Dshi_2462 at 2613.759 kb on - strand, within Dshi_2462 at 2613.772 kb on - strand, within Dshi_2462 at 2613.777 kb on - strand, within Dshi_2462 at 2613.828 kb on - strand, within Dshi_2462 at 2613.830 kb on + strand, within Dshi_2462 at 2613.830 kb on + strand, within Dshi_2462 at 2613.833 kb on - strand, within Dshi_2462 at 2613.887 kb on + strand, within Dshi_2462 at 2613.928 kb on - strand, within Dshi_2462 at 2614.014 kb on - strand, within Dshi_2462 at 2614.169 kb on + strand, within Dshi_2462 at 2614.185 kb on - strand, within Dshi_2462 at 2614.201 kb on + strand, within Dshi_2462 at 2614.204 kb on - strand, within Dshi_2462 at 2614.277 kb on + strand, within Dshi_2462 at 2614.280 kb on - strand, within Dshi_2462 at 2614.338 kb on + strand, within Dshi_2462 at 2614.359 kb on + strand, within Dshi_2462 at 2614.372 kb on - strand, within Dshi_2462 at 2614.461 kb on - strand, within Dshi_2462 at 2614.465 kb on + strand, within Dshi_2462 at 2614.539 kb on - strand, within Dshi_2462 at 2614.568 kb on - strand at 2614.573 kb on - strand at 2614.657 kb on + strand at 2614.662 kb on + strand at 2614.677 kb on + strand at 2614.823 kb on - strand, within Dshi_2463 at 2614.825 kb on + strand, within Dshi_2463 at 2614.826 kb on + strand, within Dshi_2463 at 2614.833 kb on - strand, within Dshi_2463 at 2614.833 kb on - strand, within Dshi_2463 at 2614.843 kb on - strand, within Dshi_2463 at 2614.918 kb on + strand, within Dshi_2463 at 2615.009 kb on - strand, within Dshi_2463 at 2615.010 kb on + strand, within Dshi_2463 at 2615.018 kb on - strand, within Dshi_2463 at 2615.057 kb on + strand, within Dshi_2463 at 2615.065 kb on - strand, within Dshi_2463 at 2615.075 kb on - strand, within Dshi_2463 at 2615.242 kb on - strand, within Dshi_2463 at 2615.293 kb on - strand, within Dshi_2463 at 2615.293 kb on - strand, within Dshi_2463 at 2615.410 kb on - strand at 2615.486 kb on + strand at 2615.486 kb on + strand at 2615.494 kb on - strand at 2615.510 kb on + strand at 2615.566 kb on - strand at 2615.583 kb on - strand at 2615.613 kb on + strand at 2615.652 kb on - strand, within Dshi_2464 at 2615.687 kb on - strand, within Dshi_2464
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium lysine 0.75 mM remove 2,611,953 + -0.3 2,612,164 + +0.6 2,612,165 + +0.4 2,612,201 - +0.5 2,612,207 + +0.2 2,612,340 - Dshi_2460 0.38 -0.5 2,612,403 - Dshi_2460 0.52 +1.9 2,612,437 - Dshi_2460 0.60 +0.9 2,612,523 + Dshi_2460 0.79 -1.8 2,612,536 + Dshi_2460 0.82 -0.2 2,612,544 - Dshi_2460 0.84 -0.2 2,612,584 - -0.6 2,612,730 + Dshi_2461 0.36 +0.6 2,612,865 + Dshi_2461 0.81 +0.5 2,612,870 + Dshi_2461 0.83 -1.6 2,612,871 - Dshi_2461 0.83 -0.1 2,612,871 - Dshi_2461 0.83 -0.9 2,612,886 + Dshi_2461 0.88 +0.3 2,612,894 - +0.6 2,612,910 + -0.3 2,612,967 - -0.2 2,613,039 + +0.4 2,613,069 + -0.3 2,613,076 + +1.7 2,613,091 - +0.0 2,613,121 - Dshi_2462 0.11 +0.3 2,613,151 - Dshi_2462 0.12 +0.4 2,613,169 - Dshi_2462 0.13 -0.2 2,613,202 - Dshi_2462 0.15 -0.5 2,613,267 - Dshi_2462 0.19 +0.6 2,613,344 + Dshi_2462 0.23 -0.2 2,613,386 + Dshi_2462 0.25 -1.3 2,613,396 + Dshi_2462 0.26 -0.2 2,613,425 - Dshi_2462 0.28 -0.7 2,613,538 + Dshi_2462 0.34 +0.1 2,613,582 - Dshi_2462 0.36 -0.6 2,613,610 - Dshi_2462 0.38 -0.9 2,613,695 + Dshi_2462 0.43 -0.1 2,613,737 - Dshi_2462 0.45 +0.2 2,613,738 + Dshi_2462 0.45 +0.1 2,613,738 - Dshi_2462 0.45 +0.0 2,613,759 - Dshi_2462 0.46 -0.5 2,613,772 - Dshi_2462 0.47 -1.0 2,613,777 - Dshi_2462 0.47 -1.1 2,613,828 - Dshi_2462 0.50 -1.8 2,613,830 + Dshi_2462 0.50 -0.2 2,613,830 + Dshi_2462 0.50 +0.2 2,613,833 - Dshi_2462 0.50 -0.6 2,613,887 + Dshi_2462 0.53 -0.5 2,613,928 - Dshi_2462 0.56 +0.4 2,614,014 - Dshi_2462 0.61 -0.5 2,614,169 + Dshi_2462 0.69 +1.2 2,614,185 - Dshi_2462 0.70 -0.5 2,614,201 + Dshi_2462 0.71 +0.4 2,614,204 - Dshi_2462 0.71 -0.5 2,614,277 + Dshi_2462 0.75 +0.3 2,614,280 - Dshi_2462 0.75 -0.7 2,614,338 + Dshi_2462 0.79 +0.2 2,614,359 + Dshi_2462 0.80 -0.8 2,614,372 - Dshi_2462 0.81 +0.1 2,614,461 - Dshi_2462 0.86 -1.0 2,614,465 + Dshi_2462 0.86 -0.6 2,614,539 - Dshi_2462 0.90 -0.1 2,614,568 - -0.9 2,614,573 - -0.9 2,614,657 + -0.2 2,614,662 + +0.0 2,614,677 + +0.1 2,614,823 - Dshi_2463 0.17 -0.6 2,614,825 + Dshi_2463 0.17 +0.1 2,614,826 + Dshi_2463 0.17 +0.2 2,614,833 - Dshi_2463 0.18 +0.2 2,614,833 - Dshi_2463 0.18 -0.2 2,614,843 - Dshi_2463 0.20 +0.1 2,614,918 + Dshi_2463 0.31 +0.0 2,615,009 - Dshi_2463 0.45 -0.2 2,615,010 + Dshi_2463 0.45 -0.1 2,615,018 - Dshi_2463 0.46 -0.6 2,615,057 + Dshi_2463 0.52 -0.3 2,615,065 - Dshi_2463 0.53 -0.3 2,615,075 - Dshi_2463 0.55 -0.7 2,615,242 - Dshi_2463 0.80 -0.9 2,615,293 - Dshi_2463 0.88 -0.1 2,615,293 - Dshi_2463 0.88 +0.2 2,615,410 - -0.1 2,615,486 + +0.6 2,615,486 + -0.3 2,615,494 - -0.1 2,615,510 + -0.2 2,615,566 - +0.5 2,615,583 - +0.1 2,615,613 + +0.4 2,615,652 - Dshi_2464 0.13 -0.1 2,615,687 - Dshi_2464 0.20 -0.3
Or see this region's nucleotide sequence