Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24245 and BWI76_RS24250 are separated by 204 nucleotides BWI76_RS24250 and BWI76_RS24255 are separated by 60 nucleotides BWI76_RS24255 and BWI76_RS24260 are separated by 35 nucleotides
BWI76_RS24245: BWI76_RS24245 - bifunctional glutathionylspermidine amidase/glutathionylspermidine synthase, at 5,052,296 to 5,054,167
_RS24245
BWI76_RS24250: BWI76_RS24250 - hypothetical protein, at 5,054,372 to 5,054,932
_RS24250
BWI76_RS24255: BWI76_RS24255 - thiol:disulfide oxidoreductase, at 5,054,993 to 5,055,859
_RS24255
BWI76_RS24260: BWI76_RS24260 - hypothetical protein, at 5,055,895 to 5,056,182
_RS24260
Position (kb) 5054
5055 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5053.497 kb on + strand, within BWI76_RS24245 at 5053.673 kb on - strand, within BWI76_RS24245 at 5053.819 kb on - strand, within BWI76_RS24245 at 5053.824 kb on - strand, within BWI76_RS24245 at 5054.009 kb on + strand at 5054.117 kb on + strand at 5054.117 kb on + strand at 5054.125 kb on - strand at 5054.125 kb on - strand at 5054.274 kb on - strand at 5054.407 kb on + strand at 5054.415 kb on - strand at 5054.438 kb on - strand, within BWI76_RS24250 at 5054.452 kb on + strand, within BWI76_RS24250 at 5054.485 kb on + strand, within BWI76_RS24250 at 5054.636 kb on - strand, within BWI76_RS24250 at 5054.676 kb on + strand, within BWI76_RS24250 at 5055.025 kb on - strand at 5055.447 kb on + strand, within BWI76_RS24255 at 5055.549 kb on + strand, within BWI76_RS24255 at 5055.751 kb on - strand, within BWI76_RS24255 at 5055.774 kb on + strand at 5055.918 kb on + strand at 5055.926 kb on - strand, within BWI76_RS24260
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 5,053,497 + BWI76_RS24245 0.64 -0.5 5,053,673 - BWI76_RS24245 0.74 +1.4 5,053,819 - BWI76_RS24245 0.81 +0.0 5,053,824 - BWI76_RS24245 0.82 -0.4 5,054,009 + -1.3 5,054,117 + -1.6 5,054,117 + -0.9 5,054,125 - +0.1 5,054,125 - -0.9 5,054,274 - +0.9 5,054,407 + -2.4 5,054,415 - -2.3 5,054,438 - BWI76_RS24250 0.12 +0.5 5,054,452 + BWI76_RS24250 0.14 -0.1 5,054,485 + BWI76_RS24250 0.20 -0.5 5,054,636 - BWI76_RS24250 0.47 +0.2 5,054,676 + BWI76_RS24250 0.54 -0.2 5,055,025 - +0.3 5,055,447 + BWI76_RS24255 0.52 +0.2 5,055,549 + BWI76_RS24255 0.64 +0.5 5,055,751 - BWI76_RS24255 0.87 +0.2 5,055,774 + +3.3 5,055,918 + -0.5 5,055,926 - BWI76_RS24260 0.11 +0.5
Or see this region's nucleotide sequence