Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05975 and BWI76_RS05980 overlap by 8 nucleotides BWI76_RS05980 and BWI76_RS05985 overlap by 14 nucleotides BWI76_RS05985 and BWI76_RS05990 overlap by 1 nucleotides BWI76_RS05990 and BWI76_RS05995 are separated by 20 nucleotides
BWI76_RS05975: BWI76_RS05975 - ABC transporter ATP-binding protein, at 1,254,035 to 1,254,730
_RS05975
BWI76_RS05980: BWI76_RS05980 - ABC transporter ATP-binding protein, at 1,254,723 to 1,255,505
_RS05980
BWI76_RS05985: BWI76_RS05985 - branched-chain amino acid ABC transporter permease, at 1,255,492 to 1,256,541
_RS05985
BWI76_RS05990: BWI76_RS05990 - branched-chain amino acid ABC transporter permease, at 1,256,541 to 1,257,440
_RS05990
BWI76_RS05995: BWI76_RS05995 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,257,461 to 1,258,597
_RS05995
Position (kb)
1255
1256
1257 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1254.675 kb on + strand at 1254.794 kb on - strand at 1254.816 kb on - strand, within BWI76_RS05980 at 1254.856 kb on + strand, within BWI76_RS05980 at 1255.055 kb on - strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.182 kb on + strand, within BWI76_RS05980 at 1255.338 kb on - strand, within BWI76_RS05980 at 1255.352 kb on + strand at 1255.384 kb on + strand, within BWI76_RS05980 at 1255.465 kb on + strand at 1255.831 kb on + strand, within BWI76_RS05985 at 1256.049 kb on - strand, within BWI76_RS05985 at 1256.193 kb on + strand, within BWI76_RS05985 at 1256.366 kb on - strand, within BWI76_RS05985 at 1256.382 kb on - strand, within BWI76_RS05985 at 1256.441 kb on - strand at 1256.501 kb on - strand at 1256.713 kb on - strand, within BWI76_RS05990 at 1256.756 kb on + strand, within BWI76_RS05990 at 1256.775 kb on + strand, within BWI76_RS05990 at 1256.786 kb on + strand, within BWI76_RS05990 at 1256.809 kb on + strand, within BWI76_RS05990 at 1256.809 kb on + strand, within BWI76_RS05990 at 1256.889 kb on + strand, within BWI76_RS05990 at 1257.244 kb on - strand, within BWI76_RS05990 at 1257.336 kb on + strand, within BWI76_RS05990 at 1257.396 kb on - strand at 1257.524 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 1,254,675 + +0.2 1,254,794 - -1.7 1,254,816 - BWI76_RS05980 0.12 -1.0 1,254,856 + BWI76_RS05980 0.17 +1.4 1,255,055 - BWI76_RS05980 0.42 +0.2 1,255,099 + BWI76_RS05980 0.48 -0.4 1,255,099 + BWI76_RS05980 0.48 -1.1 1,255,182 + BWI76_RS05980 0.59 -0.8 1,255,338 - BWI76_RS05980 0.79 -0.9 1,255,352 + +0.4 1,255,384 + BWI76_RS05980 0.84 -0.7 1,255,465 + -1.0 1,255,831 + BWI76_RS05985 0.32 -1.8 1,256,049 - BWI76_RS05985 0.53 -1.4 1,256,193 + BWI76_RS05985 0.67 -0.6 1,256,366 - BWI76_RS05985 0.83 -1.7 1,256,382 - BWI76_RS05985 0.85 -0.3 1,256,441 - -1.5 1,256,501 - -0.8 1,256,713 - BWI76_RS05990 0.19 -3.6 1,256,756 + BWI76_RS05990 0.24 -2.4 1,256,775 + BWI76_RS05990 0.26 -1.5 1,256,786 + BWI76_RS05990 0.27 -0.2 1,256,809 + BWI76_RS05990 0.30 -1.5 1,256,809 + BWI76_RS05990 0.30 -1.4 1,256,889 + BWI76_RS05990 0.39 -0.7 1,257,244 - BWI76_RS05990 0.78 -0.3 1,257,336 + BWI76_RS05990 0.88 -1.6 1,257,396 - -1.3 1,257,524 + -1.4
Or see this region's nucleotide sequence