Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02530 and BWI76_RS02535 are separated by 92 nucleotides BWI76_RS02535 and BWI76_RS02540 are separated by 75 nucleotides BWI76_RS02540 and BWI76_RS02545 are separated by 65 nucleotides
BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864
_RS02530
BWI76_RS02535: BWI76_RS02535 - RNA-binding protein Hfq, at 519,957 to 520,265
_RS02535
BWI76_RS02540: BWI76_RS02540 - GTPase HflX, at 520,341 to 521,621
_RS02540
BWI76_RS02545: BWI76_RS02545 - protease modulator HflK, at 521,687 to 522,943
_RS02545
Position (kb) 520
521
522 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 519.530 kb on + strand, within BWI76_RS02530 at 519.776 kb on + strand at 519.853 kb on + strand at 519.882 kb on + strand at 519.922 kb on + strand at 520.239 kb on + strand at 520.333 kb on + strand at 520.365 kb on - strand at 520.427 kb on - strand at 520.528 kb on - strand, within BWI76_RS02540 at 520.794 kb on - strand, within BWI76_RS02540 at 520.799 kb on - strand, within BWI76_RS02540 at 520.825 kb on - strand, within BWI76_RS02540 at 520.849 kb on + strand, within BWI76_RS02540 at 521.003 kb on + strand, within BWI76_RS02540 at 521.011 kb on - strand, within BWI76_RS02540 at 521.252 kb on + strand, within BWI76_RS02540 at 521.311 kb on + strand, within BWI76_RS02540 at 521.357 kb on + strand, within BWI76_RS02540 at 521.441 kb on + strand, within BWI76_RS02540 at 521.516 kb on - strand at 521.541 kb on + strand at 521.550 kb on - strand at 521.684 kb on + strand at 522.172 kb on - strand, within BWI76_RS02545 at 522.186 kb on - strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.383 kb on - strand, within BWI76_RS02545 at 522.576 kb on - strand, within BWI76_RS02545 at 522.592 kb on + strand, within BWI76_RS02545
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 519,530 + BWI76_RS02530 0.65 -3.7 519,776 + -2.8 519,853 + -0.2 519,882 + -1.8 519,922 + -0.3 520,239 + -3.4 520,333 + +1.1 520,365 - -1.9 520,427 - +0.1 520,528 - BWI76_RS02540 0.15 -0.5 520,794 - BWI76_RS02540 0.35 -1.3 520,799 - BWI76_RS02540 0.36 -2.1 520,825 - BWI76_RS02540 0.38 -1.4 520,849 + BWI76_RS02540 0.40 -0.2 521,003 + BWI76_RS02540 0.52 -0.3 521,011 - BWI76_RS02540 0.52 -1.8 521,252 + BWI76_RS02540 0.71 -1.4 521,311 + BWI76_RS02540 0.76 -1.0 521,357 + BWI76_RS02540 0.79 -0.5 521,441 + BWI76_RS02540 0.86 -0.1 521,516 - -1.2 521,541 + -0.2 521,550 - -0.4 521,684 + -1.1 522,172 - BWI76_RS02545 0.39 -1.4 522,186 - BWI76_RS02545 0.40 -0.9 522,375 + BWI76_RS02545 0.55 -1.2 522,375 + BWI76_RS02545 0.55 -1.4 522,375 + BWI76_RS02545 0.55 -0.5 522,383 - BWI76_RS02545 0.55 +0.0 522,576 - BWI76_RS02545 0.71 -0.5 522,592 + BWI76_RS02545 0.72 -1.0
Or see this region's nucleotide sequence