Experiment: reconstituted MRS with ISOThrive-prebiotic lot A 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14120 and BBR_RS14125 are separated by 150 nucleotides BBR_RS14125 and BBR_RS14130 are separated by 90 nucleotides BBR_RS14130 and BBR_RS14135 overlap by 4 nucleotides
BBR_RS14120: BBR_RS14120 - aldo/keto reductase, at 945,348 to 946,259
_RS14120
BBR_RS14125: BBR_RS14125 - phosphopyruvate hydratase, at 946,410 to 947,708
_RS14125
BBR_RS14130: BBR_RS14130 - septum formation initiator family protein, at 947,799 to 948,383
_RS14130
BBR_RS14135: BBR_RS14135 - DUF501 domain-containing protein, at 948,380 to 948,946
_RS14135
Position (kb) 946
947
948 Strain fitness (log2 ratio) -2
-1
0
1 at 945.456 kb on + strand, within BBR_RS14120 at 945.464 kb on - strand, within BBR_RS14120 at 945.472 kb on - strand, within BBR_RS14120 at 945.474 kb on + strand, within BBR_RS14120 at 945.474 kb on + strand, within BBR_RS14120 at 945.479 kb on + strand, within BBR_RS14120 at 945.546 kb on + strand, within BBR_RS14120 at 945.614 kb on + strand, within BBR_RS14120 at 945.632 kb on + strand, within BBR_RS14120 at 945.657 kb on + strand, within BBR_RS14120 at 945.657 kb on + strand, within BBR_RS14120 at 945.665 kb on - strand, within BBR_RS14120 at 945.737 kb on + strand, within BBR_RS14120 at 945.782 kb on - strand, within BBR_RS14120 at 945.797 kb on - strand, within BBR_RS14120 at 945.797 kb on - strand, within BBR_RS14120 at 945.812 kb on - strand, within BBR_RS14120 at 945.840 kb on + strand, within BBR_RS14120 at 945.845 kb on + strand, within BBR_RS14120 at 945.845 kb on + strand, within BBR_RS14120 at 945.853 kb on - strand, within BBR_RS14120 at 945.943 kb on + strand, within BBR_RS14120 at 945.953 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.987 kb on - strand, within BBR_RS14120 at 945.989 kb on + strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.125 kb on - strand, within BBR_RS14120 at 946.131 kb on - strand, within BBR_RS14120 at 946.268 kb on + strand at 946.337 kb on + strand at 946.345 kb on - strand at 946.346 kb on - strand at 947.718 kb on + strand at 948.219 kb on + strand, within BBR_RS14130 at 948.321 kb on + strand, within BBR_RS14130 at 948.353 kb on + strand at 948.353 kb on + strand at 948.358 kb on + strand at 948.366 kb on - strand at 948.378 kb on - strand at 948.403 kb on + strand at 948.403 kb on + strand at 948.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with ISOThrive-prebiotic lot A 0.5 %w/v remove 945,456 + BBR_RS14120 0.12 -0.2 945,464 - BBR_RS14120 0.13 +0.1 945,472 - BBR_RS14120 0.14 +0.7 945,474 + BBR_RS14120 0.14 +0.8 945,474 + BBR_RS14120 0.14 -0.2 945,479 + BBR_RS14120 0.14 +0.1 945,546 + BBR_RS14120 0.22 +1.1 945,614 + BBR_RS14120 0.29 +0.6 945,632 + BBR_RS14120 0.31 -0.3 945,657 + BBR_RS14120 0.34 -1.3 945,657 + BBR_RS14120 0.34 +0.4 945,665 - BBR_RS14120 0.35 -0.8 945,737 + BBR_RS14120 0.43 -0.8 945,782 - BBR_RS14120 0.48 +0.8 945,797 - BBR_RS14120 0.49 +1.4 945,797 - BBR_RS14120 0.49 +0.1 945,812 - BBR_RS14120 0.51 +0.8 945,840 + BBR_RS14120 0.54 -0.4 945,845 + BBR_RS14120 0.54 +1.3 945,845 + BBR_RS14120 0.54 +0.5 945,853 - BBR_RS14120 0.55 -0.7 945,943 + BBR_RS14120 0.65 -0.5 945,953 + BBR_RS14120 0.66 +0.6 945,979 + BBR_RS14120 0.69 -1.1 945,979 + BBR_RS14120 0.69 +1.5 945,979 + BBR_RS14120 0.69 +0.6 945,987 - BBR_RS14120 0.70 +1.3 945,989 + BBR_RS14120 0.70 +0.8 946,101 - BBR_RS14120 0.83 -0.6 946,101 - BBR_RS14120 0.83 +0.4 946,101 - BBR_RS14120 0.83 +0.4 946,125 - BBR_RS14120 0.85 -1.3 946,131 - BBR_RS14120 0.86 +0.0 946,268 + -0.3 946,337 + -0.6 946,345 - +0.6 946,346 - -1.2 947,718 + -0.5 948,219 + BBR_RS14130 0.72 +0.5 948,321 + BBR_RS14130 0.89 +0.8 948,353 + -0.5 948,353 + -0.3 948,358 + -1.8 948,366 - -0.3 948,378 - -1.6 948,403 + +0.0 948,403 + +0.2 948,411 - +0.3
Or see this region's nucleotide sequence