Experiment: reconstituted MRS with ISOThrive-prebiotic lot A 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13245 and BBR_RS13250 overlap by 20 nucleotides BBR_RS13250 and BBR_RS13255 are separated by 234 nucleotides BBR_RS13255 and BBR_RS13260 are separated by 102 nucleotides
BBR_RS13245: BBR_RS13245 - LacI family transcriptional regulator, at 745,242 to 746,486
_RS13245
BBR_RS13250: BBR_RS13250 - alpha-glucosidase, at 746,467 to 748,281
_RS13250
BBR_RS13255: BBR_RS13255 - hypothetical protein, at 748,516 to 748,692
_RS13255
BBR_RS13260: BBR_RS13260 - ABC transporter substrate-binding protein, at 748,795 to 750,414
_RS13260
Position (kb) 746
747
748
749 Strain fitness (log2 ratio) -2
-1
0
1
2 at 745.488 kb on - strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.493 kb on + strand, within BBR_RS13245 at 745.501 kb on - strand, within BBR_RS13245 at 745.525 kb on + strand, within BBR_RS13245 at 745.527 kb on + strand, within BBR_RS13245 at 745.562 kb on - strand, within BBR_RS13245 at 745.584 kb on + strand, within BBR_RS13245 at 745.592 kb on - strand, within BBR_RS13245 at 745.594 kb on - strand, within BBR_RS13245 at 745.594 kb on - strand, within BBR_RS13245 at 745.602 kb on - strand, within BBR_RS13245 at 745.607 kb on - strand, within BBR_RS13245 at 745.620 kb on + strand, within BBR_RS13245 at 745.628 kb on - strand, within BBR_RS13245 at 745.674 kb on + strand, within BBR_RS13245 at 745.725 kb on - strand, within BBR_RS13245 at 745.759 kb on + strand, within BBR_RS13245 at 745.769 kb on + strand, within BBR_RS13245 at 745.777 kb on - strand, within BBR_RS13245 at 745.777 kb on - strand, within BBR_RS13245 at 745.779 kb on + strand, within BBR_RS13245 at 745.779 kb on + strand, within BBR_RS13245 at 745.829 kb on + strand, within BBR_RS13245 at 745.854 kb on + strand, within BBR_RS13245 at 745.862 kb on - strand, within BBR_RS13245 at 745.879 kb on + strand, within BBR_RS13245 at 745.893 kb on + strand, within BBR_RS13245 at 745.952 kb on + strand, within BBR_RS13245 at 745.952 kb on + strand, within BBR_RS13245 at 745.953 kb on + strand, within BBR_RS13245 at 745.960 kb on - strand, within BBR_RS13245 at 745.972 kb on - strand, within BBR_RS13245 at 745.976 kb on + strand, within BBR_RS13245 at 745.994 kb on + strand, within BBR_RS13245 at 746.021 kb on - strand, within BBR_RS13245 at 746.049 kb on + strand, within BBR_RS13245 at 746.077 kb on + strand, within BBR_RS13245 at 746.128 kb on + strand, within BBR_RS13245 at 746.133 kb on + strand, within BBR_RS13245 at 746.148 kb on + strand, within BBR_RS13245 at 746.157 kb on - strand, within BBR_RS13245 at 746.210 kb on + strand, within BBR_RS13245 at 746.210 kb on + strand, within BBR_RS13245 at 746.223 kb on + strand, within BBR_RS13245 at 746.238 kb on - strand, within BBR_RS13245 at 746.259 kb on + strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.308 kb on + strand, within BBR_RS13245 at 746.364 kb on + strand at 746.398 kb on + strand at 746.458 kb on - strand at 746.466 kb on + strand at 746.466 kb on + strand at 746.481 kb on - strand at 746.488 kb on + strand at 746.488 kb on + strand at 746.496 kb on - strand at 746.530 kb on - strand at 746.553 kb on + strand at 746.564 kb on + strand at 746.575 kb on + strand at 746.608 kb on - strand at 746.656 kb on + strand, within BBR_RS13250 at 746.661 kb on + strand, within BBR_RS13250 at 746.661 kb on + strand, within BBR_RS13250 at 746.669 kb on - strand, within BBR_RS13250 at 746.674 kb on - strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.679 kb on + strand, within BBR_RS13250 at 746.679 kb on + strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.712 kb on - strand, within BBR_RS13250 at 746.758 kb on - strand, within BBR_RS13250 at 746.770 kb on - strand, within BBR_RS13250 at 746.792 kb on - strand, within BBR_RS13250 at 746.792 kb on - strand, within BBR_RS13250 at 746.832 kb on - strand, within BBR_RS13250 at 746.865 kb on + strand, within BBR_RS13250 at 746.865 kb on + strand, within BBR_RS13250 at 746.873 kb on - strand, within BBR_RS13250 at 746.887 kb on + strand, within BBR_RS13250 at 746.895 kb on - strand, within BBR_RS13250 at 746.899 kb on - strand, within BBR_RS13250 at 746.899 kb on - strand, within BBR_RS13250 at 746.939 kb on + strand, within BBR_RS13250 at 746.959 kb on + strand, within BBR_RS13250 at 746.985 kb on + strand, within BBR_RS13250 at 746.993 kb on + strand, within BBR_RS13250 at 746.993 kb on - strand, within BBR_RS13250 at 747.015 kb on - strand, within BBR_RS13250 at 747.022 kb on - strand, within BBR_RS13250 at 747.043 kb on + strand, within BBR_RS13250 at 747.051 kb on - strand, within BBR_RS13250 at 747.072 kb on - strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.087 kb on - strand, within BBR_RS13250 at 747.131 kb on - strand, within BBR_RS13250 at 747.142 kb on + strand, within BBR_RS13250 at 747.145 kb on - strand, within BBR_RS13250 at 747.184 kb on + strand, within BBR_RS13250 at 747.194 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.231 kb on - strand, within BBR_RS13250 at 747.233 kb on + strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.237 kb on - strand, within BBR_RS13250 at 747.252 kb on - strand, within BBR_RS13250 at 747.252 kb on - strand, within BBR_RS13250 at 747.257 kb on - strand, within BBR_RS13250 at 747.263 kb on + strand, within BBR_RS13250 at 747.322 kb on - strand, within BBR_RS13250 at 747.408 kb on + strand, within BBR_RS13250 at 747.418 kb on + strand, within BBR_RS13250 at 747.432 kb on - strand, within BBR_RS13250 at 747.518 kb on + strand, within BBR_RS13250 at 747.531 kb on + strand, within BBR_RS13250 at 747.536 kb on + strand, within BBR_RS13250 at 747.541 kb on + strand, within BBR_RS13250 at 747.544 kb on - strand, within BBR_RS13250 at 747.606 kb on + strand, within BBR_RS13250 at 747.623 kb on + strand, within BBR_RS13250 at 747.631 kb on - strand, within BBR_RS13250 at 747.649 kb on + strand, within BBR_RS13250 at 747.657 kb on + strand, within BBR_RS13250 at 747.665 kb on - strand, within BBR_RS13250 at 747.737 kb on - strand, within BBR_RS13250 at 747.752 kb on + strand, within BBR_RS13250 at 747.825 kb on + strand, within BBR_RS13250 at 747.828 kb on - strand, within BBR_RS13250 at 747.845 kb on + strand, within BBR_RS13250 at 747.877 kb on + strand, within BBR_RS13250 at 747.880 kb on - strand, within BBR_RS13250 at 747.885 kb on - strand, within BBR_RS13250 at 747.896 kb on - strand, within BBR_RS13250 at 747.947 kb on - strand, within BBR_RS13250 at 747.966 kb on + strand, within BBR_RS13250 at 747.974 kb on + strand, within BBR_RS13250 at 747.974 kb on - strand, within BBR_RS13250 at 747.982 kb on - strand, within BBR_RS13250 at 747.996 kb on + strand, within BBR_RS13250 at 748.008 kb on + strand, within BBR_RS13250 at 748.016 kb on - strand, within BBR_RS13250 at 748.022 kb on - strand, within BBR_RS13250 at 748.058 kb on + strand, within BBR_RS13250 at 748.083 kb on + strand, within BBR_RS13250 at 748.086 kb on - strand, within BBR_RS13250 at 748.086 kb on - strand, within BBR_RS13250 at 748.091 kb on - strand, within BBR_RS13250 at 748.148 kb on - strand at 748.157 kb on - strand at 748.213 kb on - strand at 748.218 kb on + strand at 748.236 kb on + strand at 748.239 kb on - strand at 748.243 kb on - strand at 748.244 kb on + strand at 748.244 kb on - strand at 748.308 kb on + strand at 748.308 kb on + strand at 748.414 kb on + strand at 748.415 kb on + strand at 748.428 kb on - strand at 748.428 kb on - strand at 748.471 kb on + strand at 748.471 kb on + strand at 748.471 kb on + strand at 748.471 kb on + strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.479 kb on - strand at 748.491 kb on + strand at 748.499 kb on - strand at 748.499 kb on - strand at 748.552 kb on - strand, within BBR_RS13255 at 748.573 kb on + strand, within BBR_RS13255 at 748.588 kb on - strand, within BBR_RS13255 at 748.618 kb on + strand, within BBR_RS13255 at 748.618 kb on + strand, within BBR_RS13255 at 748.618 kb on - strand, within BBR_RS13255 at 748.642 kb on - strand, within BBR_RS13255 at 748.654 kb on + strand, within BBR_RS13255 at 748.657 kb on - strand, within BBR_RS13255 at 748.662 kb on - strand, within BBR_RS13255 at 748.671 kb on - strand, within BBR_RS13255 at 748.834 kb on + strand at 748.842 kb on + strand at 748.897 kb on + strand at 748.946 kb on - strand at 748.953 kb on + strand at 748.981 kb on + strand, within BBR_RS13260 at 748.996 kb on + strand, within BBR_RS13260 at 749.013 kb on - strand, within BBR_RS13260 at 749.023 kb on - strand, within BBR_RS13260 at 749.023 kb on - strand, within BBR_RS13260 at 749.058 kb on - strand, within BBR_RS13260 at 749.078 kb on + strand, within BBR_RS13260 at 749.097 kb on + strand, within BBR_RS13260 at 749.115 kb on + strand, within BBR_RS13260 at 749.146 kb on - strand, within BBR_RS13260 at 749.146 kb on - strand, within BBR_RS13260 at 749.158 kb on + strand, within BBR_RS13260 at 749.158 kb on + strand, within BBR_RS13260 at 749.165 kb on - strand, within BBR_RS13260 at 749.181 kb on + strand, within BBR_RS13260 at 749.201 kb on - strand, within BBR_RS13260 at 749.244 kb on - strand, within BBR_RS13260 at 749.244 kb on - strand, within BBR_RS13260 at 749.276 kb on - strand, within BBR_RS13260 at 749.276 kb on - strand, within BBR_RS13260
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with ISOThrive-prebiotic lot A 0.5 %w/v remove 745,488 - BBR_RS13245 0.20 -0.8 745,492 + BBR_RS13245 0.20 +0.4 745,492 + BBR_RS13245 0.20 -0.5 745,492 + BBR_RS13245 0.20 -0.5 745,493 + BBR_RS13245 0.20 +0.1 745,501 - BBR_RS13245 0.21 -1.4 745,525 + BBR_RS13245 0.23 +0.3 745,527 + BBR_RS13245 0.23 +0.3 745,562 - BBR_RS13245 0.26 +0.2 745,584 + BBR_RS13245 0.27 +0.6 745,592 - BBR_RS13245 0.28 -1.1 745,594 - BBR_RS13245 0.28 +0.2 745,594 - BBR_RS13245 0.28 -0.7 745,602 - BBR_RS13245 0.29 +0.4 745,607 - BBR_RS13245 0.29 +0.2 745,620 + BBR_RS13245 0.30 -0.2 745,628 - BBR_RS13245 0.31 -0.5 745,674 + BBR_RS13245 0.35 -0.3 745,725 - BBR_RS13245 0.39 +0.4 745,759 + BBR_RS13245 0.42 -1.4 745,769 + BBR_RS13245 0.42 +0.5 745,777 - BBR_RS13245 0.43 -0.8 745,777 - BBR_RS13245 0.43 -0.2 745,779 + BBR_RS13245 0.43 -0.3 745,779 + BBR_RS13245 0.43 -0.2 745,829 + BBR_RS13245 0.47 +0.5 745,854 + BBR_RS13245 0.49 +0.8 745,862 - BBR_RS13245 0.50 +0.2 745,879 + BBR_RS13245 0.51 -1.4 745,893 + BBR_RS13245 0.52 -0.7 745,952 + BBR_RS13245 0.57 -0.4 745,952 + BBR_RS13245 0.57 +0.3 745,953 + BBR_RS13245 0.57 -0.1 745,960 - BBR_RS13245 0.58 -0.6 745,972 - BBR_RS13245 0.59 -0.1 745,976 + BBR_RS13245 0.59 +0.2 745,994 + BBR_RS13245 0.60 +0.3 746,021 - BBR_RS13245 0.63 +0.5 746,049 + BBR_RS13245 0.65 -0.3 746,077 + BBR_RS13245 0.67 +0.1 746,128 + BBR_RS13245 0.71 -0.7 746,133 + BBR_RS13245 0.72 +0.4 746,148 + BBR_RS13245 0.73 -0.2 746,157 - BBR_RS13245 0.73 -0.5 746,210 + BBR_RS13245 0.78 -0.2 746,210 + BBR_RS13245 0.78 +0.1 746,223 + BBR_RS13245 0.79 +0.1 746,238 - BBR_RS13245 0.80 +0.4 746,259 + BBR_RS13245 0.82 -0.2 746,290 - BBR_RS13245 0.84 -0.5 746,290 - BBR_RS13245 0.84 +0.5 746,290 - BBR_RS13245 0.84 -0.9 746,308 + BBR_RS13245 0.86 -0.9 746,364 + +0.9 746,398 + -0.1 746,458 - -0.8 746,466 + -0.2 746,466 + +0.7 746,481 - -0.1 746,488 + +0.8 746,488 + +0.4 746,496 - +0.4 746,530 - +0.3 746,553 + -0.0 746,564 + -1.8 746,575 + +0.2 746,608 - +0.4 746,656 + BBR_RS13250 0.10 -0.3 746,661 + BBR_RS13250 0.11 +0.7 746,661 + BBR_RS13250 0.11 +0.6 746,669 - BBR_RS13250 0.11 -1.6 746,674 - BBR_RS13250 0.11 -0.0 746,677 + BBR_RS13250 0.12 -0.9 746,677 + BBR_RS13250 0.12 +0.0 746,677 + BBR_RS13250 0.12 +0.0 746,679 + BBR_RS13250 0.12 +0.1 746,679 + BBR_RS13250 0.12 +0.6 746,685 - BBR_RS13250 0.12 +1.3 746,685 - BBR_RS13250 0.12 +1.1 746,685 - BBR_RS13250 0.12 +0.2 746,712 - BBR_RS13250 0.13 -0.1 746,758 - BBR_RS13250 0.16 +0.7 746,770 - BBR_RS13250 0.17 -0.4 746,792 - BBR_RS13250 0.18 -0.1 746,792 - BBR_RS13250 0.18 +0.3 746,832 - BBR_RS13250 0.20 +0.3 746,865 + BBR_RS13250 0.22 -0.6 746,865 + BBR_RS13250 0.22 -1.0 746,873 - BBR_RS13250 0.22 +1.2 746,887 + BBR_RS13250 0.23 -0.2 746,895 - BBR_RS13250 0.24 -0.6 746,899 - BBR_RS13250 0.24 +0.7 746,899 - BBR_RS13250 0.24 +0.3 746,939 + BBR_RS13250 0.26 -0.0 746,959 + BBR_RS13250 0.27 +0.0 746,985 + BBR_RS13250 0.29 -1.2 746,993 + BBR_RS13250 0.29 +0.3 746,993 - BBR_RS13250 0.29 +1.1 747,015 - BBR_RS13250 0.30 +0.8 747,022 - BBR_RS13250 0.31 -0.3 747,043 + BBR_RS13250 0.32 +0.6 747,051 - BBR_RS13250 0.32 +0.2 747,072 - BBR_RS13250 0.33 -0.6 747,079 + BBR_RS13250 0.34 -0.8 747,079 + BBR_RS13250 0.34 +1.8 747,079 + BBR_RS13250 0.34 -0.5 747,079 + BBR_RS13250 0.34 +0.5 747,079 + BBR_RS13250 0.34 -0.5 747,079 + BBR_RS13250 0.34 +0.6 747,079 + BBR_RS13250 0.34 -0.4 747,087 - BBR_RS13250 0.34 -0.3 747,131 - BBR_RS13250 0.37 +1.0 747,142 + BBR_RS13250 0.37 +0.0 747,145 - BBR_RS13250 0.37 -0.2 747,184 + BBR_RS13250 0.40 +1.0 747,194 + BBR_RS13250 0.40 +0.3 747,228 + BBR_RS13250 0.42 +0.1 747,228 + BBR_RS13250 0.42 -0.6 747,228 + BBR_RS13250 0.42 -0.7 747,231 - BBR_RS13250 0.42 +0.9 747,233 + BBR_RS13250 0.42 -0.1 747,236 - BBR_RS13250 0.42 +0.4 747,236 - BBR_RS13250 0.42 -0.3 747,236 - BBR_RS13250 0.42 +0.7 747,237 - BBR_RS13250 0.42 -1.6 747,252 - BBR_RS13250 0.43 +0.9 747,252 - BBR_RS13250 0.43 +0.1 747,257 - BBR_RS13250 0.44 +0.2 747,263 + BBR_RS13250 0.44 -0.1 747,322 - BBR_RS13250 0.47 +0.3 747,408 + BBR_RS13250 0.52 -0.5 747,418 + BBR_RS13250 0.52 -0.3 747,432 - BBR_RS13250 0.53 -0.1 747,518 + BBR_RS13250 0.58 +1.0 747,531 + BBR_RS13250 0.59 +0.5 747,536 + BBR_RS13250 0.59 -0.2 747,541 + BBR_RS13250 0.59 +0.9 747,544 - BBR_RS13250 0.59 +2.3 747,606 + BBR_RS13250 0.63 -0.6 747,623 + BBR_RS13250 0.64 -0.4 747,631 - BBR_RS13250 0.64 -1.7 747,649 + BBR_RS13250 0.65 -0.6 747,657 + BBR_RS13250 0.66 -0.1 747,665 - BBR_RS13250 0.66 -0.2 747,737 - BBR_RS13250 0.70 +0.1 747,752 + BBR_RS13250 0.71 -0.6 747,825 + BBR_RS13250 0.75 -0.3 747,828 - BBR_RS13250 0.75 -0.0 747,845 + BBR_RS13250 0.76 -0.4 747,877 + BBR_RS13250 0.78 +0.8 747,880 - BBR_RS13250 0.78 +0.6 747,885 - BBR_RS13250 0.78 -0.1 747,896 - BBR_RS13250 0.79 +0.6 747,947 - BBR_RS13250 0.82 -0.9 747,966 + BBR_RS13250 0.83 -0.6 747,974 + BBR_RS13250 0.83 -0.0 747,974 - BBR_RS13250 0.83 +0.8 747,982 - BBR_RS13250 0.83 -0.2 747,996 + BBR_RS13250 0.84 +0.1 748,008 + BBR_RS13250 0.85 +0.4 748,016 - BBR_RS13250 0.85 -0.2 748,022 - BBR_RS13250 0.86 -0.6 748,058 + BBR_RS13250 0.88 -0.2 748,083 + BBR_RS13250 0.89 +0.1 748,086 - BBR_RS13250 0.89 -2.4 748,086 - BBR_RS13250 0.89 -1.2 748,091 - BBR_RS13250 0.89 -0.2 748,148 - -1.2 748,157 - -1.7 748,213 - +0.5 748,218 + +0.6 748,236 + -0.3 748,239 - -0.7 748,243 - +0.9 748,244 + -0.3 748,244 - -0.0 748,308 + -0.2 748,308 + -0.2 748,414 + +1.3 748,415 + +0.2 748,428 - -0.7 748,428 - +0.5 748,471 + -0.0 748,471 + +0.6 748,471 + +0.5 748,471 + +0.4 748,479 - +0.1 748,479 - -0.1 748,479 - +1.3 748,479 - +0.9 748,479 - +0.1 748,491 + +0.1 748,499 - +0.1 748,499 - +0.5 748,552 - BBR_RS13255 0.20 +0.5 748,573 + BBR_RS13255 0.32 +1.0 748,588 - BBR_RS13255 0.41 -0.9 748,618 + BBR_RS13255 0.58 -0.1 748,618 + BBR_RS13255 0.58 -2.0 748,618 - BBR_RS13255 0.58 +0.5 748,642 - BBR_RS13255 0.71 +0.4 748,654 + BBR_RS13255 0.78 +0.2 748,657 - BBR_RS13255 0.80 -0.5 748,662 - BBR_RS13255 0.82 +0.4 748,671 - BBR_RS13255 0.88 +0.6 748,834 + -0.4 748,842 + -1.8 748,897 + -0.5 748,946 - -0.1 748,953 + +1.4 748,981 + BBR_RS13260 0.11 +0.1 748,996 + BBR_RS13260 0.12 -0.4 749,013 - BBR_RS13260 0.13 +1.2 749,023 - BBR_RS13260 0.14 -0.4 749,023 - BBR_RS13260 0.14 +0.2 749,058 - BBR_RS13260 0.16 +1.2 749,078 + BBR_RS13260 0.17 +0.3 749,097 + BBR_RS13260 0.19 +0.6 749,115 + BBR_RS13260 0.20 -0.3 749,146 - BBR_RS13260 0.22 -0.3 749,146 - BBR_RS13260 0.22 -0.5 749,158 + BBR_RS13260 0.22 +0.0 749,158 + BBR_RS13260 0.22 +0.3 749,165 - BBR_RS13260 0.23 -0.8 749,181 + BBR_RS13260 0.24 -0.6 749,201 - BBR_RS13260 0.25 -0.1 749,244 - BBR_RS13260 0.28 +2.2 749,244 - BBR_RS13260 0.28 -0.5 749,276 - BBR_RS13260 0.30 -0.6 749,276 - BBR_RS13260 0.30 +0.5
Or see this region's nucleotide sequence