Experiment: reconstituted MRS with ISOThrive-prebiotic lot A 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12180 and BBR_RS12185 are separated by 269 nucleotides BBR_RS12185 and BBR_RS12190 are separated by 289 nucleotides BBR_RS12190 and BBR_RS12195 are separated by 164 nucleotides
BBR_RS12180: BBR_RS12180 - DUF2974 domain-containing protein, at 481,393 to 482,730
_RS12180
BBR_RS12185: BBR_RS12185 - methylated-DNA--[protein]-cysteine S-methyltransferase, at 483,000 to 483,554
_RS12185
BBR_RS12190: BBR_RS12190 - holo-ACP synthase, at 483,844 to 484,293
_RS12190
BBR_RS12195: BBR_RS12195 - alpha-amylase, at 484,458 to 485,000
_RS12195
Position (kb) 482
483
484 Strain fitness (log2 ratio) -2
-1
0
1
2 at 482.010 kb on - strand, within BBR_RS12180 at 482.010 kb on - strand, within BBR_RS12180 at 482.054 kb on + strand, within BBR_RS12180 at 482.062 kb on - strand, within BBR_RS12180 at 482.076 kb on + strand, within BBR_RS12180 at 482.112 kb on + strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.169 kb on + strand, within BBR_RS12180 at 482.170 kb on - strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.214 kb on + strand, within BBR_RS12180 at 482.232 kb on + strand, within BBR_RS12180 at 482.237 kb on + strand, within BBR_RS12180 at 482.240 kb on - strand, within BBR_RS12180 at 482.246 kb on - strand, within BBR_RS12180 at 482.255 kb on - strand, within BBR_RS12180 at 482.303 kb on + strand, within BBR_RS12180 at 482.305 kb on + strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.347 kb on + strand, within BBR_RS12180 at 482.347 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.378 kb on + strand, within BBR_RS12180 at 482.380 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.386 kb on - strand, within BBR_RS12180 at 482.388 kb on + strand, within BBR_RS12180 at 482.388 kb on + strand, within BBR_RS12180 at 482.390 kb on - strand, within BBR_RS12180 at 482.391 kb on - strand, within BBR_RS12180 at 482.409 kb on + strand, within BBR_RS12180 at 482.410 kb on + strand, within BBR_RS12180 at 482.410 kb on + strand, within BBR_RS12180 at 482.415 kb on - strand, within BBR_RS12180 at 482.433 kb on - strand, within BBR_RS12180 at 482.478 kb on + strand, within BBR_RS12180 at 482.478 kb on + strand, within BBR_RS12180 at 482.478 kb on - strand, within BBR_RS12180 at 482.486 kb on - strand, within BBR_RS12180 at 482.496 kb on - strand, within BBR_RS12180 at 482.524 kb on - strand, within BBR_RS12180 at 482.524 kb on - strand, within BBR_RS12180 at 482.538 kb on - strand, within BBR_RS12180 at 482.544 kb on + strand, within BBR_RS12180 at 482.557 kb on - strand, within BBR_RS12180 at 482.589 kb on - strand, within BBR_RS12180 at 482.604 kb on + strand at 482.604 kb on + strand at 482.604 kb on + strand at 482.604 kb on + strand at 482.604 kb on + strand at 482.609 kb on + strand at 482.612 kb on - strand at 482.622 kb on - strand at 482.622 kb on - strand at 482.622 kb on - strand at 482.706 kb on + strand at 482.714 kb on - strand at 482.726 kb on + strand at 482.726 kb on + strand at 482.750 kb on + strand at 482.771 kb on - strand at 482.789 kb on - strand at 482.798 kb on + strand at 482.820 kb on + strand at 482.829 kb on + strand at 482.857 kb on + strand at 482.871 kb on - strand at 482.871 kb on - strand at 482.884 kb on - strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.933 kb on + strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.938 kb on - strand at 482.943 kb on - strand at 482.943 kb on - strand at 482.997 kb on + strand at 482.997 kb on + strand at 483.005 kb on - strand at 483.016 kb on - strand at 483.039 kb on + strand at 483.039 kb on + strand at 483.046 kb on - strand at 483.047 kb on + strand at 483.051 kb on + strand at 483.052 kb on + strand at 483.054 kb on - strand at 483.060 kb on - strand, within BBR_RS12185 at 483.075 kb on - strand, within BBR_RS12185 at 483.107 kb on + strand, within BBR_RS12185 at 483.112 kb on + strand, within BBR_RS12185 at 483.141 kb on - strand, within BBR_RS12185 at 483.141 kb on - strand, within BBR_RS12185 at 483.147 kb on + strand, within BBR_RS12185 at 483.164 kb on + strand, within BBR_RS12185 at 483.164 kb on - strand, within BBR_RS12185 at 483.169 kb on + strand, within BBR_RS12185 at 483.187 kb on + strand, within BBR_RS12185 at 483.216 kb on - strand, within BBR_RS12185 at 483.226 kb on - strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.245 kb on + strand, within BBR_RS12185 at 483.293 kb on + strand, within BBR_RS12185 at 483.329 kb on + strand, within BBR_RS12185 at 483.335 kb on + strand, within BBR_RS12185 at 483.336 kb on + strand, within BBR_RS12185 at 483.343 kb on - strand, within BBR_RS12185 at 483.355 kb on + strand, within BBR_RS12185 at 483.363 kb on - strand, within BBR_RS12185 at 483.368 kb on - strand, within BBR_RS12185 at 483.370 kb on - strand, within BBR_RS12185 at 483.380 kb on - strand, within BBR_RS12185 at 483.384 kb on + strand, within BBR_RS12185 at 483.386 kb on + strand, within BBR_RS12185 at 483.398 kb on + strand, within BBR_RS12185 at 483.409 kb on + strand, within BBR_RS12185 at 483.425 kb on - strand, within BBR_RS12185 at 483.450 kb on - strand, within BBR_RS12185 at 483.460 kb on + strand, within BBR_RS12185 at 483.463 kb on - strand, within BBR_RS12185 at 483.463 kb on - strand, within BBR_RS12185 at 483.485 kb on - strand, within BBR_RS12185 at 483.486 kb on + strand, within BBR_RS12185 at 483.500 kb on + strand at 483.551 kb on - strand at 483.621 kb on + strand at 483.639 kb on + strand at 483.639 kb on + strand at 483.647 kb on - strand at 483.654 kb on - strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.695 kb on - strand at 483.718 kb on - strand at 483.758 kb on + strand at 483.763 kb on + strand at 483.805 kb on + strand at 483.813 kb on - strand at 483.825 kb on + strand at 483.833 kb on - strand at 484.324 kb on + strand at 484.334 kb on - strand at 484.353 kb on + strand at 484.395 kb on + strand at 484.396 kb on + strand at 484.403 kb on - strand at 484.420 kb on + strand at 484.453 kb on + strand at 484.459 kb on - strand at 484.466 kb on + strand at 484.492 kb on - strand at 484.511 kb on + strand at 484.511 kb on + strand at 484.518 kb on + strand, within BBR_RS12195 at 484.518 kb on + strand, within BBR_RS12195 at 484.518 kb on + strand, within BBR_RS12195 at 484.544 kb on + strand, within BBR_RS12195 at 484.550 kb on - strand, within BBR_RS12195 at 484.551 kb on + strand, within BBR_RS12195 at 484.552 kb on - strand, within BBR_RS12195 at 484.552 kb on - strand, within BBR_RS12195 at 484.552 kb on - strand, within BBR_RS12195 at 484.554 kb on - strand, within BBR_RS12195
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with ISOThrive-prebiotic lot A 0.5 %w/v remove 482,010 - BBR_RS12180 0.46 -0.7 482,010 - BBR_RS12180 0.46 -0.1 482,054 + BBR_RS12180 0.49 -0.2 482,062 - BBR_RS12180 0.50 -0.5 482,076 + BBR_RS12180 0.51 -0.2 482,112 + BBR_RS12180 0.54 -0.9 482,120 - BBR_RS12180 0.54 +0.1 482,120 - BBR_RS12180 0.54 -0.6 482,120 - BBR_RS12180 0.54 +0.9 482,139 + BBR_RS12180 0.56 +1.1 482,139 + BBR_RS12180 0.56 +0.4 482,139 + BBR_RS12180 0.56 -0.0 482,139 + BBR_RS12180 0.56 +0.1 482,147 - BBR_RS12180 0.56 -0.4 482,147 - BBR_RS12180 0.56 -0.4 482,147 - BBR_RS12180 0.56 +0.0 482,147 - BBR_RS12180 0.56 -0.4 482,147 - BBR_RS12180 0.56 +0.2 482,169 + BBR_RS12180 0.58 +0.0 482,170 - BBR_RS12180 0.58 -0.5 482,202 + BBR_RS12180 0.60 -0.2 482,202 + BBR_RS12180 0.60 +0.3 482,214 + BBR_RS12180 0.61 +0.6 482,232 + BBR_RS12180 0.63 -1.2 482,237 + BBR_RS12180 0.63 +0.9 482,240 - BBR_RS12180 0.63 +0.6 482,246 - BBR_RS12180 0.64 -0.1 482,255 - BBR_RS12180 0.64 -0.2 482,303 + BBR_RS12180 0.68 -0.8 482,305 + BBR_RS12180 0.68 +0.4 482,339 - BBR_RS12180 0.71 +1.0 482,339 - BBR_RS12180 0.71 -0.0 482,339 - BBR_RS12180 0.71 +0.7 482,339 - BBR_RS12180 0.71 -0.0 482,339 - BBR_RS12180 0.71 -0.6 482,347 + BBR_RS12180 0.71 +0.6 482,347 + BBR_RS12180 0.71 +0.5 482,376 + BBR_RS12180 0.73 -0.9 482,376 + BBR_RS12180 0.73 -0.5 482,376 + BBR_RS12180 0.73 -0.5 482,376 + BBR_RS12180 0.73 +0.6 482,376 + BBR_RS12180 0.73 -0.5 482,376 + BBR_RS12180 0.73 +0.3 482,376 + BBR_RS12180 0.73 -0.4 482,376 + BBR_RS12180 0.73 -0.2 482,376 + BBR_RS12180 0.73 -2.2 482,376 + BBR_RS12180 0.73 -0.6 482,378 + BBR_RS12180 0.74 -0.3 482,380 - BBR_RS12180 0.74 +1.9 482,384 - BBR_RS12180 0.74 +0.1 482,384 - BBR_RS12180 0.74 +1.5 482,384 - BBR_RS12180 0.74 +0.5 482,384 - BBR_RS12180 0.74 +1.1 482,384 - BBR_RS12180 0.74 -0.9 482,384 - BBR_RS12180 0.74 +0.2 482,384 - BBR_RS12180 0.74 -0.7 482,384 - BBR_RS12180 0.74 +2.1 482,384 - BBR_RS12180 0.74 -0.3 482,386 - BBR_RS12180 0.74 -0.7 482,388 + BBR_RS12180 0.74 +0.6 482,388 + BBR_RS12180 0.74 -0.2 482,390 - BBR_RS12180 0.75 +0.5 482,391 - BBR_RS12180 0.75 -1.0 482,409 + BBR_RS12180 0.76 -0.2 482,410 + BBR_RS12180 0.76 +0.2 482,410 + BBR_RS12180 0.76 -0.0 482,415 - BBR_RS12180 0.76 +0.5 482,433 - BBR_RS12180 0.78 -0.2 482,478 + BBR_RS12180 0.81 -0.4 482,478 + BBR_RS12180 0.81 +1.3 482,478 - BBR_RS12180 0.81 -0.9 482,486 - BBR_RS12180 0.82 -0.8 482,496 - BBR_RS12180 0.82 -0.4 482,524 - BBR_RS12180 0.85 +0.3 482,524 - BBR_RS12180 0.85 +0.3 482,538 - BBR_RS12180 0.86 -1.6 482,544 + BBR_RS12180 0.86 +0.0 482,557 - BBR_RS12180 0.87 +1.0 482,589 - BBR_RS12180 0.89 -0.3 482,604 + -0.4 482,604 + -2.1 482,604 + +1.5 482,604 + -0.6 482,604 + +0.5 482,609 + +1.5 482,612 - +0.1 482,622 - -0.7 482,622 - +0.5 482,622 - +0.1 482,706 + +0.1 482,714 - +1.0 482,726 + -0.2 482,726 + -0.1 482,750 + +1.2 482,771 - +0.6 482,789 - -0.5 482,798 + -2.5 482,820 + -0.3 482,829 + -0.4 482,857 + -0.4 482,871 - +1.2 482,871 - +0.8 482,884 - +0.5 482,928 + +0.6 482,928 + -0.2 482,928 + +0.3 482,928 + +0.2 482,928 + +0.0 482,933 + +0.1 482,936 - +0.1 482,936 - +0.1 482,936 - -1.0 482,936 - +0.2 482,936 - +0.5 482,936 - -0.2 482,936 - +0.6 482,938 - +0.1 482,943 - +0.8 482,943 - -1.2 482,997 + +0.3 482,997 + -0.8 483,005 - -0.4 483,016 - +0.2 483,039 + +0.6 483,039 + -0.2 483,046 - -0.5 483,047 + +0.1 483,051 + -0.4 483,052 + -0.2 483,054 - +0.4 483,060 - BBR_RS12185 0.11 -0.1 483,075 - BBR_RS12185 0.14 +1.3 483,107 + BBR_RS12185 0.19 -0.0 483,112 + BBR_RS12185 0.20 +0.8 483,141 - BBR_RS12185 0.25 -0.7 483,141 - BBR_RS12185 0.25 +1.4 483,147 + BBR_RS12185 0.26 +0.9 483,164 + BBR_RS12185 0.30 -0.5 483,164 - BBR_RS12185 0.30 +0.2 483,169 + BBR_RS12185 0.30 +1.1 483,187 + BBR_RS12185 0.34 +0.5 483,216 - BBR_RS12185 0.39 -0.2 483,226 - BBR_RS12185 0.41 -0.2 483,233 + BBR_RS12185 0.42 +0.8 483,233 + BBR_RS12185 0.42 -0.0 483,233 + BBR_RS12185 0.42 +0.6 483,233 + BBR_RS12185 0.42 +0.6 483,245 + BBR_RS12185 0.44 -1.0 483,293 + BBR_RS12185 0.53 +0.0 483,329 + BBR_RS12185 0.59 +0.4 483,335 + BBR_RS12185 0.60 +0.4 483,336 + BBR_RS12185 0.61 +0.8 483,343 - BBR_RS12185 0.62 +0.4 483,355 + BBR_RS12185 0.64 -0.1 483,363 - BBR_RS12185 0.65 +0.3 483,368 - BBR_RS12185 0.66 +0.3 483,370 - BBR_RS12185 0.67 -0.2 483,380 - BBR_RS12185 0.68 +0.1 483,384 + BBR_RS12185 0.69 +0.8 483,386 + BBR_RS12185 0.70 +0.3 483,398 + BBR_RS12185 0.72 -0.2 483,409 + BBR_RS12185 0.74 -0.0 483,425 - BBR_RS12185 0.77 +0.9 483,450 - BBR_RS12185 0.81 +0.8 483,460 + BBR_RS12185 0.83 -0.2 483,463 - BBR_RS12185 0.83 +0.1 483,463 - BBR_RS12185 0.83 +0.3 483,485 - BBR_RS12185 0.87 +0.4 483,486 + BBR_RS12185 0.88 +0.3 483,500 + +0.1 483,551 - -0.5 483,621 + -0.3 483,639 + -0.2 483,639 + +1.4 483,647 - +0.3 483,654 - -0.9 483,687 + -0.6 483,687 + +0.2 483,687 + +0.4 483,687 + -0.2 483,687 + +0.3 483,695 - -0.3 483,718 - -0.1 483,758 + -0.5 483,763 + -0.9 483,805 + -0.0 483,813 - -0.6 483,825 + -0.0 483,833 - +0.7 484,324 + +0.3 484,334 - +0.2 484,353 + -0.1 484,395 + +0.5 484,396 + -0.6 484,403 - +0.8 484,420 + +1.9 484,453 + +0.7 484,459 - -0.5 484,466 + -0.3 484,492 - +0.4 484,511 + -0.1 484,511 + +0.5 484,518 + BBR_RS12195 0.11 -0.0 484,518 + BBR_RS12195 0.11 +0.9 484,518 + BBR_RS12195 0.11 +0.8 484,544 + BBR_RS12195 0.16 -1.5 484,550 - BBR_RS12195 0.17 -0.2 484,551 + BBR_RS12195 0.17 +0.3 484,552 - BBR_RS12195 0.17 -0.2 484,552 - BBR_RS12195 0.17 +0.3 484,552 - BBR_RS12195 0.17 +0.8 484,554 - BBR_RS12195 0.18 -1.0
Or see this region's nucleotide sequence