Experiment: formate McAA riboflavin dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07770 and MMP_RS07775 are separated by 56 nucleotides MMP_RS07775 and MMP_RS07780 are separated by 296 nucleotides
MMP_RS07770: MMP_RS07770 - alanine racemase, at 1,470,522 to 1,471,643
_RS07770
MMP_RS07775: MMP_RS07775 - alanine dehydrogenase, at 1,471,700 to 1,472,821
_RS07775
MMP_RS07780: MMP_RS07780 - prephenate dehydrogenase, at 1,473,118 to 1,474,458
_RS07780
Position (kb)
1471
1472
1473 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1470.853 kb on + strand, within MMP_RS07770 at 1470.853 kb on + strand, within MMP_RS07770 at 1470.895 kb on + strand, within MMP_RS07770 at 1470.966 kb on - strand, within MMP_RS07770 at 1470.967 kb on + strand, within MMP_RS07770 at 1470.984 kb on - strand, within MMP_RS07770 at 1470.989 kb on - strand, within MMP_RS07770 at 1471.117 kb on - strand, within MMP_RS07770 at 1471.657 kb on - strand at 1471.658 kb on + strand at 1472.194 kb on - strand, within MMP_RS07775 at 1472.300 kb on - strand, within MMP_RS07775 at 1472.324 kb on - strand, within MMP_RS07775 at 1472.512 kb on - strand, within MMP_RS07775 at 1472.513 kb on + strand, within MMP_RS07775 at 1472.542 kb on + strand, within MMP_RS07775 at 1472.771 kb on + strand at 1472.840 kb on + strand at 1472.876 kb on - strand at 1472.879 kb on - strand at 1472.893 kb on + strand at 1473.010 kb on - strand at 1473.010 kb on - strand at 1473.011 kb on + strand at 1473.021 kb on - strand at 1473.083 kb on - strand at 1473.084 kb on + strand at 1473.087 kb on + strand at 1473.087 kb on + strand at 1473.208 kb on - strand at 1473.236 kb on - strand at 1473.277 kb on - strand at 1473.530 kb on - strand at 1473.535 kb on + strand at 1473.535 kb on + strand at 1473.653 kb on - strand at 1473.772 kb on + strand at 1473.774 kb on - strand at 1473.806 kb on - strand at 1473.807 kb on + strand at 1473.807 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA riboflavin dropout remove 1,470,853 + MMP_RS07770 0.30 +0.2 1,470,853 + MMP_RS07770 0.30 +0.4 1,470,895 + MMP_RS07770 0.33 -0.6 1,470,966 - MMP_RS07770 0.40 +0.1 1,470,967 + MMP_RS07770 0.40 +0.3 1,470,984 - MMP_RS07770 0.41 -1.1 1,470,989 - MMP_RS07770 0.42 +0.9 1,471,117 - MMP_RS07770 0.53 +0.9 1,471,657 - -0.5 1,471,658 + +0.4 1,472,194 - MMP_RS07775 0.44 -0.9 1,472,300 - MMP_RS07775 0.53 -1.6 1,472,324 - MMP_RS07775 0.56 -0.5 1,472,512 - MMP_RS07775 0.72 +0.0 1,472,513 + MMP_RS07775 0.72 -0.1 1,472,542 + MMP_RS07775 0.75 -0.5 1,472,771 + +0.3 1,472,840 + -1.0 1,472,876 - -0.2 1,472,879 - +0.1 1,472,893 + -0.1 1,473,010 - +0.2 1,473,010 - -0.1 1,473,011 + -0.2 1,473,021 - -0.1 1,473,083 - -0.8 1,473,084 + -1.6 1,473,087 + -1.3 1,473,087 + -0.7 1,473,208 - -3.0 1,473,236 - -2.4 1,473,277 - -2.3 1,473,530 - +0.1 1,473,535 + -2.9 1,473,535 + -0.5 1,473,653 - +1.6 1,473,772 + -0.7 1,473,774 - -2.0 1,473,806 - -1.2 1,473,807 + -2.1 1,473,807 + -0.7
Or see this region's nucleotide sequence