Experiment: formate McAA riboflavin dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01740 and MMP_RS01745 are separated by 135 nucleotides MMP_RS01745 and MMP_RS01750 are separated by 109 nucleotides MMP_RS01750 and MMP_RS01755 are separated by 14 nucleotides
MMP_RS01740: MMP_RS01740 - methionine--tRNA ligase, at 322,398 to 324,389
_RS01740
MMP_RS01745: MMP_RS01745 - AMP phosphorylase, at 324,525 to 326,042
_RS01745
MMP_RS01750: MMP_RS01750 - DUF63 family protein, at 326,152 to 326,958
_RS01750
MMP_RS01755: MMP_RS01755 - DUF2097 domain-containing protein, at 326,973 to 327,263
_RS01755
Position (kb) 324
325
326
327 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 323.802 kb on - strand at 323.842 kb on - strand at 323.945 kb on + strand at 323.945 kb on + strand at 323.948 kb on - strand at 323.970 kb on + strand at 324.212 kb on + strand at 324.220 kb on + strand at 324.332 kb on - strand at 324.337 kb on - strand at 324.337 kb on - strand at 324.390 kb on - strand at 324.406 kb on - strand at 324.459 kb on - strand at 324.468 kb on - strand at 324.485 kb on - strand at 324.550 kb on - strand at 324.637 kb on - strand at 324.767 kb on + strand, within MMP_RS01745 at 324.918 kb on - strand, within MMP_RS01745 at 324.920 kb on - strand, within MMP_RS01745 at 325.025 kb on - strand, within MMP_RS01745 at 325.072 kb on - strand, within MMP_RS01745 at 325.469 kb on + strand, within MMP_RS01745 at 325.661 kb on + strand, within MMP_RS01745 at 325.929 kb on - strand at 325.972 kb on - strand at 325.972 kb on - strand at 326.019 kb on + strand at 326.058 kb on - strand at 326.060 kb on - strand at 326.060 kb on - strand at 326.061 kb on + strand at 326.061 kb on + strand at 326.065 kb on + strand at 326.081 kb on + strand at 326.087 kb on + strand at 326.116 kb on + strand at 326.131 kb on + strand at 326.194 kb on - strand at 326.195 kb on + strand at 326.227 kb on + strand at 326.268 kb on + strand, within MMP_RS01750 at 326.338 kb on + strand, within MMP_RS01750 at 326.445 kb on - strand, within MMP_RS01750 at 326.445 kb on - strand, within MMP_RS01750 at 326.449 kb on - strand, within MMP_RS01750 at 326.450 kb on + strand, within MMP_RS01750 at 326.501 kb on + strand, within MMP_RS01750 at 326.501 kb on + strand, within MMP_RS01750 at 326.505 kb on + strand, within MMP_RS01750 at 326.585 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.590 kb on - strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.591 kb on + strand, within MMP_RS01750 at 326.603 kb on - strand, within MMP_RS01750 at 326.613 kb on + strand, within MMP_RS01750 at 326.617 kb on - strand, within MMP_RS01750 at 326.620 kb on - strand, within MMP_RS01750 at 326.620 kb on - strand, within MMP_RS01750 at 326.621 kb on + strand, within MMP_RS01750 at 326.633 kb on + strand, within MMP_RS01750 at 326.837 kb on - strand, within MMP_RS01750 at 326.881 kb on + strand at 327.036 kb on - strand, within MMP_RS01755
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA riboflavin dropout remove 323,802 - -3.1 323,842 - -0.6 323,945 + -1.1 323,945 + -0.5 323,948 - -2.0 323,970 + -2.2 324,212 + -2.6 324,220 + -0.8 324,332 - -3.2 324,337 - -1.3 324,337 - -0.8 324,390 - -0.4 324,406 - +0.0 324,459 - +0.3 324,468 - -0.2 324,485 - +0.2 324,550 - -0.8 324,637 - -0.6 324,767 + MMP_RS01745 0.16 -1.0 324,918 - MMP_RS01745 0.26 -0.3 324,920 - MMP_RS01745 0.26 -0.6 325,025 - MMP_RS01745 0.33 -1.1 325,072 - MMP_RS01745 0.36 -1.2 325,469 + MMP_RS01745 0.62 +0.3 325,661 + MMP_RS01745 0.75 -0.9 325,929 - -0.6 325,972 - -0.7 325,972 - -0.5 326,019 + -0.1 326,058 - -0.6 326,060 - +0.2 326,060 - -0.5 326,061 + +0.2 326,061 + -0.2 326,065 + -0.2 326,081 + -0.5 326,087 + -1.0 326,116 + +0.3 326,131 + -0.2 326,194 - -0.1 326,195 + -0.4 326,227 + +0.1 326,268 + MMP_RS01750 0.14 +0.2 326,338 + MMP_RS01750 0.23 +0.1 326,445 - MMP_RS01750 0.36 -0.1 326,445 - MMP_RS01750 0.36 -0.3 326,449 - MMP_RS01750 0.37 -2.8 326,450 + MMP_RS01750 0.37 -0.7 326,501 + MMP_RS01750 0.43 -0.2 326,501 + MMP_RS01750 0.43 -0.1 326,505 + MMP_RS01750 0.44 -0.2 326,585 - MMP_RS01750 0.54 -1.5 326,590 - MMP_RS01750 0.54 -0.8 326,590 - MMP_RS01750 0.54 -0.2 326,590 - MMP_RS01750 0.54 -0.6 326,590 - MMP_RS01750 0.54 -1.1 326,590 - MMP_RS01750 0.54 -0.5 326,591 + MMP_RS01750 0.54 -0.8 326,591 + MMP_RS01750 0.54 -0.2 326,591 + MMP_RS01750 0.54 -0.4 326,603 - MMP_RS01750 0.56 -0.5 326,613 + MMP_RS01750 0.57 +0.1 326,617 - MMP_RS01750 0.58 -0.7 326,620 - MMP_RS01750 0.58 -0.7 326,620 - MMP_RS01750 0.58 +0.2 326,621 + MMP_RS01750 0.58 +0.3 326,633 + MMP_RS01750 0.60 +0.0 326,837 - MMP_RS01750 0.85 -0.6 326,881 + -0.3 327,036 - MMP_RS01755 0.22 -2.4
Or see this region's nucleotide sequence