Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_818 and HP15_819 are separated by 41 nucleotides HP15_819 and HP15_820 are separated by 3 nucleotides HP15_820 and HP15_821 are separated by 153 nucleotides
HP15_818: HP15_818 - protein containing diguanylate phosphodiesterase, predicted domains, at 836,746 to 837,453
_818
HP15_819: HP15_819 - conserved hypothetical protein, membrane, at 837,495 to 837,839
_819
HP15_820: HP15_820 - transcriptional regulator, LysR family protein, at 837,843 to 838,736
_820
HP15_821: HP15_821 - acyl-CoA dehydrogenase family protein, at 838,890 to 840,101
_821
Position (kb)
837
838
839 Strain fitness (log2 ratio)
-2
-1
0
1 at 836.943 kb on - strand, within HP15_818 at 836.992 kb on + strand, within HP15_818 at 836.993 kb on - strand, within HP15_818 at 837.030 kb on + strand, within HP15_818 at 837.090 kb on - strand, within HP15_818 at 837.136 kb on - strand, within HP15_818 at 837.209 kb on - strand, within HP15_818 at 837.243 kb on - strand, within HP15_818 at 837.321 kb on + strand, within HP15_818 at 837.393 kb on + strand at 837.481 kb on - strand at 837.654 kb on - strand, within HP15_819 at 837.853 kb on + strand at 837.966 kb on - strand, within HP15_820 at 838.088 kb on - strand, within HP15_820 at 838.103 kb on + strand, within HP15_820 at 838.237 kb on + strand, within HP15_820 at 838.725 kb on + strand at 838.733 kb on - strand at 838.822 kb on + strand at 838.822 kb on + strand at 838.822 kb on + strand at 838.830 kb on - strand at 838.830 kb on - strand at 838.842 kb on + strand at 839.009 kb on + strand at 839.119 kb on - strand, within HP15_821 at 839.271 kb on + strand, within HP15_821 at 839.276 kb on + strand, within HP15_821 at 839.487 kb on - strand, within HP15_821 at 839.487 kb on - strand, within HP15_821 at 839.493 kb on + strand, within HP15_821 at 839.564 kb on - strand, within HP15_821 at 839.653 kb on + strand, within HP15_821
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 836,943 - HP15_818 0.28 -0.1 836,992 + HP15_818 0.35 -0.4 836,993 - HP15_818 0.35 -0.3 837,030 + HP15_818 0.40 +0.1 837,090 - HP15_818 0.49 +0.3 837,136 - HP15_818 0.55 -0.2 837,209 - HP15_818 0.65 +0.7 837,243 - HP15_818 0.70 +0.5 837,321 + HP15_818 0.81 -1.5 837,393 + +1.5 837,481 - -0.9 837,654 - HP15_819 0.46 -1.5 837,853 + -1.9 837,966 - HP15_820 0.14 +1.5 838,088 - HP15_820 0.27 -0.2 838,103 + HP15_820 0.29 -1.0 838,237 + HP15_820 0.44 -0.7 838,725 + -1.0 838,733 - +0.1 838,822 + +1.5 838,822 + +1.0 838,822 + +0.3 838,830 - -0.5 838,830 - -1.1 838,842 + -0.5 839,009 + -0.7 839,119 - HP15_821 0.19 +0.8 839,271 + HP15_821 0.31 +0.1 839,276 + HP15_821 0.32 +1.2 839,487 - HP15_821 0.49 +0.5 839,487 - HP15_821 0.49 +0.3 839,493 + HP15_821 0.50 +1.3 839,564 - HP15_821 0.56 +0.8 839,653 + HP15_821 0.63 -0.5
Or see this region's nucleotide sequence