Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_780 and HP15_781 are separated by 104 nucleotides HP15_781 and HP15_782 are separated by 12 nucleotides HP15_782 and HP15_783 are separated by 26 nucleotides
HP15_780: HP15_780 - sensory box/GGDEF family protein, at 790,020 to 792,770
_780
HP15_781: HP15_781 - GTP-binding protein LepA, at 792,875 to 794,677
_781
HP15_782: HP15_782 - signal peptidase I, at 794,690 to 795,496
_782
HP15_783: HP15_783 - conserved hypothetical protein, at 795,523 to 795,912
_783
Position (kb) 792
793
794
795 Strain fitness (log2 ratio) -1
0
1 at 791.886 kb on - strand, within HP15_780 at 791.969 kb on + strand, within HP15_780 at 792.060 kb on + strand, within HP15_780 at 792.123 kb on + strand, within HP15_780 at 792.194 kb on + strand, within HP15_780 at 792.204 kb on - strand, within HP15_780 at 792.209 kb on - strand, within HP15_780 at 792.301 kb on + strand, within HP15_780 at 792.328 kb on - strand, within HP15_780 at 792.357 kb on + strand, within HP15_780 at 792.615 kb on - strand at 792.637 kb on + strand at 792.637 kb on + strand at 792.637 kb on + strand at 792.688 kb on - strand at 793.001 kb on + strand at 793.066 kb on + strand, within HP15_781 at 793.066 kb on + strand, within HP15_781 at 793.066 kb on + strand, within HP15_781 at 793.196 kb on + strand, within HP15_781 at 793.763 kb on + strand, within HP15_781 at 794.211 kb on + strand, within HP15_781
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 791,886 - HP15_780 0.68 +0.5 791,969 + HP15_780 0.71 -0.5 792,060 + HP15_780 0.74 -0.0 792,123 + HP15_780 0.76 +0.7 792,194 + HP15_780 0.79 +0.1 792,204 - HP15_780 0.79 +0.3 792,209 - HP15_780 0.80 -0.8 792,301 + HP15_780 0.83 +0.0 792,328 - HP15_780 0.84 +1.0 792,357 + HP15_780 0.85 +0.9 792,615 - +0.8 792,637 + -0.6 792,637 + -1.5 792,637 + -0.1 792,688 - +0.2 793,001 + -1.2 793,066 + HP15_781 0.11 -0.9 793,066 + HP15_781 0.11 -0.7 793,066 + HP15_781 0.11 -0.8 793,196 + HP15_781 0.18 -1.3 793,763 + HP15_781 0.49 -0.3 794,211 + HP15_781 0.74 +0.3
Or see this region's nucleotide sequence