Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_770 and HP15_771 are separated by 110 nucleotides HP15_771 and HP15_772 are separated by 21 nucleotides HP15_772 and HP15_773 overlap by 17 nucleotides HP15_773 and HP15_774 are separated by 39 nucleotides
HP15_770: HP15_770 - signal transduction protein, at 781,148 to 781,981
_770
HP15_771: HP15_771 - glycine cleavage T-protein (aminomethyl transferase), at 782,092 to 783,087
_771
HP15_772: HP15_772 - conserved hypothetical protein, secreted, at 783,109 to 783,573
_772
HP15_773: HP15_773 - conserved hypothetical protein, at 783,557 to 783,829
_773
HP15_774: HP15_774 - L-aspartate oxidase, at 783,869 to 785,494
_774
Position (kb) 782
783
784 Strain fitness (log2 ratio) -1
0
1
2 at 781.519 kb on - strand, within HP15_770 at 781.572 kb on + strand, within HP15_770 at 781.622 kb on + strand, within HP15_770 at 781.673 kb on + strand, within HP15_770 at 781.709 kb on + strand, within HP15_770 at 781.709 kb on + strand, within HP15_770 at 781.717 kb on - strand, within HP15_770 at 781.724 kb on - strand, within HP15_770 at 781.878 kb on + strand, within HP15_770 at 781.878 kb on + strand, within HP15_770 at 781.985 kb on + strand at 781.987 kb on + strand at 781.987 kb on + strand at 781.990 kb on - strand at 781.990 kb on - strand at 782.132 kb on + strand at 782.431 kb on + strand, within HP15_771 at 782.567 kb on - strand, within HP15_771 at 782.651 kb on + strand, within HP15_771 at 782.868 kb on - strand, within HP15_771 at 783.138 kb on + strand at 783.306 kb on + strand, within HP15_772 at 783.306 kb on + strand, within HP15_772 at 783.314 kb on - strand, within HP15_772 at 783.382 kb on + strand, within HP15_772 at 783.390 kb on - strand, within HP15_772 at 783.412 kb on - strand, within HP15_772 at 783.417 kb on - strand, within HP15_772 at 783.417 kb on - strand, within HP15_772 at 783.551 kb on - strand at 783.564 kb on + strand at 783.839 kb on - strand at 783.915 kb on - strand at 783.917 kb on - strand at 783.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 781,519 - HP15_770 0.44 +0.7 781,572 + HP15_770 0.51 +1.6 781,622 + HP15_770 0.57 -0.0 781,673 + HP15_770 0.63 -0.1 781,709 + HP15_770 0.67 +2.6 781,709 + HP15_770 0.67 -0.4 781,717 - HP15_770 0.68 -0.2 781,724 - HP15_770 0.69 +0.5 781,878 + HP15_770 0.88 -0.4 781,878 + HP15_770 0.88 -0.5 781,985 + -0.9 781,987 + +1.0 781,987 + -0.4 781,990 - +0.0 781,990 - +0.1 782,132 + +1.8 782,431 + HP15_771 0.34 -1.5 782,567 - HP15_771 0.48 -0.7 782,651 + HP15_771 0.56 -0.6 782,868 - HP15_771 0.78 +0.0 783,138 + +0.4 783,306 + HP15_772 0.42 +0.1 783,306 + HP15_772 0.42 +0.0 783,314 - HP15_772 0.44 -0.1 783,382 + HP15_772 0.59 +0.3 783,390 - HP15_772 0.60 +0.2 783,412 - HP15_772 0.65 +0.9 783,417 - HP15_772 0.66 +1.4 783,417 - HP15_772 0.66 -0.3 783,551 - +0.2 783,564 + -0.3 783,839 - -0.1 783,915 - -0.5 783,917 - -0.5 783,998 - +0.1
Or see this region's nucleotide sequence