Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_520 and HP15_521 are separated by 66 nucleotides HP15_521 and HP15_522 are separated by 6 nucleotides HP15_522 and HP15_523 are separated by 95 nucleotides
HP15_520: HP15_520 - type IV pilus biogenesis protein, at 531,968 to 535,465
_520
HP15_521: HP15_521 - fimbrial protein pilin, at 535,532 to 535,894
_521
HP15_522: HP15_522 - type IV pilus biogenesis protein-like protein, at 535,901 to 536,458
_522
HP15_523: HP15_523 - two component, sigma54 specific, transcriptional regulator, Fis family, at 536,554 to 537,942
_523
Position (kb) 535
536
537 Strain fitness (log2 ratio) -1
0
1
2 at 534.911 kb on + strand, within HP15_520 at 534.967 kb on + strand, within HP15_520 at 534.975 kb on - strand, within HP15_520 at 534.993 kb on + strand, within HP15_520 at 534.993 kb on + strand, within HP15_520 at 535.101 kb on + strand, within HP15_520 at 535.191 kb on + strand at 535.199 kb on - strand at 535.199 kb on - strand at 535.315 kb on + strand at 535.323 kb on - strand at 535.392 kb on - strand at 535.641 kb on - strand, within HP15_521 at 535.857 kb on - strand, within HP15_521 at 535.974 kb on - strand, within HP15_522 at 536.209 kb on - strand, within HP15_522 at 536.317 kb on - strand, within HP15_522 at 536.541 kb on + strand at 536.834 kb on - strand, within HP15_523 at 536.841 kb on + strand, within HP15_523 at 536.841 kb on + strand, within HP15_523 at 536.841 kb on + strand, within HP15_523 at 536.849 kb on - strand, within HP15_523 at 536.849 kb on - strand, within HP15_523 at 536.849 kb on - strand, within HP15_523 at 536.851 kb on - strand, within HP15_523 at 536.919 kb on - strand, within HP15_523 at 536.919 kb on - strand, within HP15_523 at 537.031 kb on + strand, within HP15_523 at 537.116 kb on - strand, within HP15_523 at 537.143 kb on + strand, within HP15_523 at 537.149 kb on - strand, within HP15_523 at 537.238 kb on + strand, within HP15_523
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 534,911 + HP15_520 0.84 -0.8 534,967 + HP15_520 0.86 -1.3 534,975 - HP15_520 0.86 +1.0 534,993 + HP15_520 0.86 +0.4 534,993 + HP15_520 0.86 +1.6 535,101 + HP15_520 0.90 +1.8 535,191 + -0.1 535,199 - -1.4 535,199 - +1.4 535,315 + -0.4 535,323 - +1.3 535,392 - +0.9 535,641 - HP15_521 0.30 +0.1 535,857 - HP15_521 0.90 +0.0 535,974 - HP15_522 0.13 +0.4 536,209 - HP15_522 0.55 -1.7 536,317 - HP15_522 0.75 -1.2 536,541 + +0.5 536,834 - HP15_523 0.20 +0.2 536,841 + HP15_523 0.21 -1.4 536,841 + HP15_523 0.21 +0.9 536,841 + HP15_523 0.21 +0.2 536,849 - HP15_523 0.21 +0.3 536,849 - HP15_523 0.21 -1.3 536,849 - HP15_523 0.21 +0.2 536,851 - HP15_523 0.21 -0.3 536,919 - HP15_523 0.26 -0.2 536,919 - HP15_523 0.26 -0.4 537,031 + HP15_523 0.34 -0.0 537,116 - HP15_523 0.40 +0.4 537,143 + HP15_523 0.42 -0.6 537,149 - HP15_523 0.43 -0.4 537,238 + HP15_523 0.49 -0.4
Or see this region's nucleotide sequence