Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_425 and HP15_426 overlap by 4 nucleotides HP15_426 and HP15_427 are separated by 254 nucleotides HP15_427 and HP15_428 are separated by 173 nucleotides
HP15_425: HP15_425 - two-component response regulator NtrC, at 422,734 to 424,161
_425
HP15_426: HP15_426 - signal transduction histidine kinase, nitrogen specific, NtrB, at 424,158 to 425,252
_426
HP15_427: HP15_427 - conserved hypothetical protein, secreted, at 425,507 to 426,031
_427
HP15_428: HP15_428 - glutamine synthetase, type I, at 426,205 to 427,608
_428
Position (kb) 424
425
426 Strain fitness (log2 ratio) -2
-1
0
1 at 423.212 kb on + strand, within HP15_425 at 423.229 kb on - strand, within HP15_425 at 423.230 kb on - strand, within HP15_425 at 423.406 kb on - strand, within HP15_425 at 423.565 kb on + strand, within HP15_425 at 423.565 kb on + strand, within HP15_425 at 423.573 kb on - strand, within HP15_425 at 423.573 kb on - strand, within HP15_425 at 423.809 kb on + strand, within HP15_425 at 423.827 kb on - strand, within HP15_425 at 423.856 kb on + strand, within HP15_425 at 423.903 kb on - strand, within HP15_425 at 424.081 kb on + strand at 424.086 kb on + strand at 424.086 kb on + strand at 424.086 kb on + strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.094 kb on - strand at 424.099 kb on + strand at 424.107 kb on - strand at 424.107 kb on - strand at 424.107 kb on - strand at 424.144 kb on - strand at 424.144 kb on - strand at 424.167 kb on - strand at 424.236 kb on + strand at 424.236 kb on + strand at 424.251 kb on + strand at 424.251 kb on + strand at 424.258 kb on + strand at 424.277 kb on - strand, within HP15_426 at 424.382 kb on - strand, within HP15_426 at 424.407 kb on + strand, within HP15_426 at 424.417 kb on + strand, within HP15_426 at 424.423 kb on - strand, within HP15_426 at 424.456 kb on - strand, within HP15_426 at 424.487 kb on + strand, within HP15_426 at 424.529 kb on + strand, within HP15_426 at 424.540 kb on + strand, within HP15_426 at 424.540 kb on + strand, within HP15_426 at 424.621 kb on - strand, within HP15_426 at 424.666 kb on + strand, within HP15_426 at 424.674 kb on - strand, within HP15_426 at 424.778 kb on + strand, within HP15_426 at 424.976 kb on + strand, within HP15_426 at 425.017 kb on + strand, within HP15_426 at 425.154 kb on + strand at 425.189 kb on - strand at 425.213 kb on - strand at 425.407 kb on + strand at 425.407 kb on + strand at 425.415 kb on - strand at 425.473 kb on + strand at 425.518 kb on - strand at 425.704 kb on + strand, within HP15_427 at 425.704 kb on + strand, within HP15_427 at 425.712 kb on - strand, within HP15_427 at 425.851 kb on + strand, within HP15_427 at 425.851 kb on + strand, within HP15_427 at 425.851 kb on + strand, within HP15_427 at 425.856 kb on + strand, within HP15_427 at 425.864 kb on - strand, within HP15_427 at 425.866 kb on + strand, within HP15_427 at 425.883 kb on + strand, within HP15_427 at 425.920 kb on + strand, within HP15_427 at 425.928 kb on - strand, within HP15_427 at 425.928 kb on - strand, within HP15_427 at 425.966 kb on - strand, within HP15_427 at 426.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 423,212 + HP15_425 0.33 +0.4 423,229 - HP15_425 0.35 -0.8 423,230 - HP15_425 0.35 +0.7 423,406 - HP15_425 0.47 -0.3 423,565 + HP15_425 0.58 +0.1 423,565 + HP15_425 0.58 -0.0 423,573 - HP15_425 0.59 +0.7 423,573 - HP15_425 0.59 -0.3 423,809 + HP15_425 0.75 -0.6 423,827 - HP15_425 0.77 -0.6 423,856 + HP15_425 0.79 -0.4 423,903 - HP15_425 0.82 -0.2 424,081 + +0.1 424,086 + -1.5 424,086 + -1.3 424,086 + -0.9 424,094 - -0.1 424,094 - +0.0 424,094 - -0.3 424,094 - +0.3 424,094 - +0.3 424,099 + +0.1 424,107 - +0.2 424,107 - -0.7 424,107 - -1.8 424,144 - +0.0 424,144 - -0.5 424,167 - -0.1 424,236 + -1.9 424,236 + -1.0 424,251 + -0.8 424,251 + -0.3 424,258 + -0.7 424,277 - HP15_426 0.11 -0.4 424,382 - HP15_426 0.20 -0.0 424,407 + HP15_426 0.23 -1.4 424,417 + HP15_426 0.24 -1.2 424,423 - HP15_426 0.24 -0.6 424,456 - HP15_426 0.27 +0.8 424,487 + HP15_426 0.30 -1.9 424,529 + HP15_426 0.34 +0.2 424,540 + HP15_426 0.35 -0.4 424,540 + HP15_426 0.35 -0.0 424,621 - HP15_426 0.42 -1.1 424,666 + HP15_426 0.46 -1.8 424,674 - HP15_426 0.47 -0.9 424,778 + HP15_426 0.57 +0.7 424,976 + HP15_426 0.75 -0.3 425,017 + HP15_426 0.78 -1.1 425,154 + -2.1 425,189 - -0.2 425,213 - +1.1 425,407 + -0.4 425,407 + -0.4 425,415 - +1.2 425,473 + +1.7 425,518 - +1.7 425,704 + HP15_427 0.38 -0.7 425,704 + HP15_427 0.38 +0.4 425,712 - HP15_427 0.39 -0.2 425,851 + HP15_427 0.66 +0.5 425,851 + HP15_427 0.66 +0.1 425,851 + HP15_427 0.66 -0.2 425,856 + HP15_427 0.66 +0.6 425,864 - HP15_427 0.68 -1.1 425,866 + HP15_427 0.68 -1.1 425,883 + HP15_427 0.72 -1.3 425,920 + HP15_427 0.79 -0.3 425,928 - HP15_427 0.80 -2.5 425,928 - HP15_427 0.80 -0.3 425,966 - HP15_427 0.87 +1.0 426,167 + +1.0
Or see this region's nucleotide sequence